Studies on the production and practical use of engineered human salivary type cystatins.

工程人唾液型半胱氨酸蛋白酶抑制剂的生产和实际应用研究。

基本信息

  • 批准号:
    12671817
  • 负责人:
  • 金额:
    $ 2.11万
  • 依托单位:
  • 依托单位国家:
    日本
  • 项目类别:
    Grant-in-Aid for Scientific Research (C)
  • 财政年份:
    2000
  • 资助国家:
    日本
  • 起止时间:
    2000 至 2002
  • 项目状态:
    已结题

项目摘要

This research project focused on the viewpoint of practical use of biological activities of human salivary cystatins and natural cysteine proteinase inhibitors from plants and animals. In addition, a system enabling large-scale production of engineered cystatins at a Ievel of several hundreds to several thousands milligrams was investigated. Based on these concepts and plans, novel cysteine proteinase inhibitors were screened as well by the reverse zymography developed in this study. The results obtained are summarized as follows: (1) Recombinant cystatins produced by E. coli were demonstrated to induce interleukin-6 and interleukin-8 productions in the human gingival fibroblast cells via cell surface molecules. (2) The engineered salivary type cystatins were also found to depress the formation of axillary odors by microorganisms such as Brevibacterium. (3) Some experimental data have indicated that there are some components mat inhibit the growth of Porphyromonas gingivalis in the hea … More t-extract of the rice bran of Niigata-Koshihikari. At present, we have been studying not only oryzacystatins with molecular weight of 13 kDa but also several candidate proteins of 50-70 kDa that tightly bind to a cysteine proteinase, papain and ficin. The detail features of the substances are now under investigation by the reverse zymographical technique since rice cysteine proteinase inhibitors could be a potent inhibitor of the growth of P. gingivalis as well as salivary cystatins. (6) The mechanism of a defense system present in the skin mucus of fishes may be helpful to study physiological roles of salivary proteins in oral cavity. Hence, we have attempted to search for flie defense proteins such as proteainses, proteinase inhibitors, and the lectins in the mucus fluid of the Japanese eel. As the result, the skin mucus was shown to contain novel cysteine proteinase inhibitor, with molecular masses of about 13-15 kDa. However, it is obscure at present that the inhibitors may be the members of cystatin superfamily, or not. Taken together, it can be concluded that cysteine proteinase inhibitors from plants and animals are thought to be a considerably useful biomaterial for the maintenance of oral health and for the clinical trials of periodontitis. Less
本研究项目着眼于从动植物中提取的人唾液胱抑素和天然半胱氨酸蛋白酶抑制剂的生物活性的实际应用观点。此外,还研究了一种能够大规模生产数百至数千毫克水平的工程胱抑素的系统。基于这些概念和计划,利用本研究开发的反相酶谱法筛选了新型半胱氨酸蛋白酶抑制剂。结果表明:(1)大肠杆菌生产的重组胱他汀可通过细胞表面分子诱导人牙龈成纤维细胞产生白细胞介素-6和白细胞介素-8。(2)工程唾液型胱抑素还可抑制短杆菌等微生物对腋窝气味的形成。(3)一些实验数据表明,新泻泻光米糠提取物中存在抑制牙龈卟啉单胞菌生长的成分。目前,我们不仅在研究分子量为13 kDa的米胞他汀类药物,还在研究与半胱氨酸蛋白酶、木瓜蛋白酶和ficin紧密结合的50-70 kDa的候选蛋白。由于水稻半胱氨酸蛋白酶抑制剂可能是牙龈卟啉单胞菌和唾液胱抑素生长的有效抑制剂,因此这些物质的详细特征目前正在用反酶技术进行研究。(6)鱼类皮肤黏液中存在一种防御系统的机制可能有助于研究唾液蛋白在口腔中的生理作用。因此,我们试图在日本鳗鱼的黏液中寻找苍蝇防御蛋白,如蛋白酶、蛋白酶抑制剂和凝集素。结果表明,皮肤黏液含有新型半胱氨酸蛋白酶抑制剂,分子量约为13-15 kDa。然而,目前尚不清楚这些抑制剂是否属于胱抑素超家族成员。综上所述,可以得出结论,来自植物和动物的半胱氨酸蛋白酶抑制剂被认为是一种非常有用的生物材料,可用于维持口腔健康和牙周炎的临床试验。少

项目成果

期刊论文数量(29)
专著数量(0)
科研奖励数量(0)
会议论文数量(0)
专利数量(0)
Kato T., Imatani, T., Miura, T., Minaguchi, K., Saitoh, E., Okuda, K.: "Cytokine inducing activity of family 2 cystatins"Biological Chemistry. Vol.381. 1143-1147 (2000)
Kato T.、Imatani, T.、Miura, T.、Minaguchi, K.、Saitoh, E.、Okuda, K.:“家族 2 胱抑素的细胞因子诱导活性”生物化学。
  • DOI:
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    0
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Kato, T.j Imatani, T., Minaguchi, K., Saitoh, E., & Okuda, K.: "CystatiiK induce IL-6 production by human and murine cells."The Journal of Dental Research. Vol. 80, No. 4. 1330 (2001)
加藤,T.j 今谷,T.,皆口,K.,斋藤,E.,
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    0
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Kato, T., Imatani, T., Minaguchi, K., Saitoh, E., Okuda, K.: "Salivary cystatins induce interleukin-6 expression via cell surface molecules in human gingival fibroblasts"Molecular Immunology. Vol.39. 423-430 (2002)
Kato, T.、Imatani, T.、Minaguchi, K.、Saitoh, E.、Okuda, K.:“唾液半胱氨酸蛋白酶抑制剂通过人牙龈成纤维细胞中的细胞表面分子诱导白细胞介素 6 表达”分子免疫学。
  • DOI:
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    0
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Chiba, A., Oka, S., Saitoh, E.: "Ontogenetic changes in neuropeptide Y-immunoreactive cerebrospinal fluid-containing neurons in the hypothalamaus of the cloudy dogfish Scyliorhinus torazame (Elasmobranchii)"Neuroscience Letters. Vol.329,No.3. 301-304 (200
Chiba, A., Oka, S., Saitoh, E.:“云角鲨下丘脑中神经肽 Y 免疫反应性脑脊液神经元的个体发生变化”神经科学快报。
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    0
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SAITOH Eiichi其他文献

SAITOH Eiichi的其他文献

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{{ truncateString('SAITOH Eiichi', 18)}}的其他基金

Proteomics Analysis of Secretory Cysteine Protease Inhibitors and their Practical Application on Oral Health
分泌型半胱氨酸蛋白酶抑制剂的蛋白质组学分析及其在口腔健康中的实际应用
  • 批准号:
    15591981
  • 财政年份:
    2003
  • 资助金额:
    $ 2.11万
  • 项目类别:
    Grant-in-Aid for Scientific Research (C)
The development of Medial hip joint system for reconstruction of paraplegic locomotion
重建截瘫运动的内侧髋关节系统的研制
  • 批准号:
    12832063
  • 财政年份:
    2000
  • 资助金额:
    $ 2.11万
  • 项目类别:
    Grant-in-Aid for Scientific Research (C)
The Mdial system for reconstruction of paraplegic locomotion - its development and refinement using virtual axis and motor power assist
用于重建截瘫运动的 Mdial 系统 - 使用虚拟轴和电机动力辅助进行开发和完善
  • 批准号:
    10838044
  • 财政年份:
    1998
  • 资助金额:
    $ 2.11万
  • 项目类别:
    Grant-in-Aid for Scientific Research (C)
Studies on Production of Human Salivary Type Cystatins by Genetic Engineering and their Application to Dental Medicine
基因工程生产人唾液型胱抑素及其在牙科医学中的应用研究
  • 批准号:
    09671910
  • 财政年份:
    1997
  • 资助金额:
    $ 2.11万
  • 项目类别:
    Grant-in-Aid for Scientific Research (C)
A study on large scale production, improvement of function, and utilization of recombinant cystatins
重组半胱氨酸蛋白酶抑制剂的规模化生产、功能改良及利用研究
  • 批准号:
    06671872
  • 财政年份:
    1995
  • 资助金额:
    $ 2.11万
  • 项目类别:
    Grant-in-Aid for Scientific Research (C)
Molecular Genetic Study of the Cystatin Gene Family
半胱氨酸蛋白酶抑制剂基因家族的分子遗传学研究
  • 批准号:
    02670842
  • 财政年份:
    1990
  • 资助金额:
    $ 2.11万
  • 项目类别:
    Grant-in-Aid for General Scientific Research (C)

相似海外基金

Screening for food-grade anti-amyloidgenic compounds in food stuffs by using human cystatin C and improvement of their functionalities
利用人半胱氨酸蛋白酶抑制剂C筛选食品中的食品级抗淀粉样蛋白生成化合物并改进其功能
  • 批准号:
    18580114
  • 财政年份:
    2006
  • 资助金额:
    $ 2.11万
  • 项目类别:
    Grant-in-Aid for Scientific Research (C)
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