Bacteriorogical Study for Conservative Treatment of Pulp-Temporary Indirect Plup Capping-

本实验对以冷水痛为主的牙髓病患牙进行了临床检查，并从龋齿和牙髓的牙本质深层进行了细菌鉴定。据此，得出以下结论。1.所有病例均从牙本质深部分离到细菌。研究发现，当牙髓室打开时，细菌可以被带到根管内。因此，对于所有因龋病而进行牙髓摘除治疗的病例，在根管充填之前对根管进行细菌培养的必要性已有所不同。2.在所有从根管中分离到的细菌种类较少的病例中，分离到的细菌与从牙本质深层分离到的细菌中的一些细菌相同，这表明牙髓受到牙本质深层细菌直接侵入的影响。3.在根管内细菌种类较多的情况下，不是从牙本质深层分离的类杆菌、梭杆菌、普氏杆菌等专性厌氧菌和专性厌氧乳杆菌的分离率较高，牙髓中检出的细菌数量较多。这表明，当从牙本质深层侵入的细菌在牙髓内繁殖时，它们可能会改变类型和比例。4.如果龋齿有潮湿的深褐色感染牙本质，细菌侵入牙髓的可能性较高，这可以为确定治疗措施提供指导。

In this experiment, teeth with dental pulp disease, mainlyexperiencing cold water pain, have been clinically examined and bacteria has been identified from deep in the dentin of decayed teeth and dental pulp. Accordingly, the following are concluded. 1. Bacteria were isolated from the deep part of dentin in allcases examinaed. It was found that the bacteria could be carried to the canal while the pulp chamber is open. Thus the need for bacteriologic culturing of root canals before the canal is filled for all cases in which pulpectomy treatment has been applied to dental pulp desease caused by caries has been varified. 2. In all the cases wherein the number of bacterium types isolated from the canal was small, the isolated bacteria were identical to some of those detected from the deep part of dentin, which implies that dental pulp is affected by direct invasion into it by bacteria existing deep in dentin. 3. In instances where the number of bacterium types isolated from canal was large, obligate anaerobic such as Bacteroides, Fusobacterium, Prevotella and obligate anaerobic Lactobacillus, which were not isolated from the deep part of dentin, had a comparatively high ratio of isolation and the number of bacteria detected from the dental pulp proved to be large. This suggests the possibility that, at the stage when bacteria invading from the deep part of dentin multiply within the dental pulp, they change types and the percentages. 4. In case of a decayed tooth with a damp, dark-brown infected dentin, the possibility of bacteria invasion into the dental pulp to be relatively high, which can furnish a guideline for determining treatment measures