Changes in denture-supporting tissues under continuous pressure in osteoporosis

骨质疏松症持续压力下义齿支撑组织的变化

• 批准号: 12671884
• 负责人: MORI Shingo
• 金额: $ 2.18万
• 依托单位: OKAYAMA UNIVERSITY
• 依托单位国家: 日本
• 项目类别: Grant-in-Aid for Scientific Research (C)
• 财政年份: 2000
• 资助国家: 日本
• 起止时间: 2000 至 2001
• 项目状态: 已结题
• 来源: https://kaken.nii.ac.jp/grant/KAKENHI-PROJECT-12671884/
• 关键词: Osteoporosis; Denture-supporting tissues; Continuous pressure; AgNOR; Histopathology; Rat

In this study, the experimental denture base on the hard palate of the molar region of osteoporotic rats was designed to cover without any mechanical pressure or to load continuous pressure (0.5, 1.5 or 3.4 kPa). Experimental osteoporosis was induced by ovariectomy and feeding on a low calcium diet. The histopathological changes and the changes in the argyrophilic nucleolar organizer region (AgNOR) counts in mucosal epithelium that was revealed to the cellular activity, induced by non-pressure covering or continuous pressure in the tissues uncler the experimental denture bases were examined. Regarding the histological changes induced by continuous pressure, this was evaluated in relation to the time course of the intensity of the continuous pressure. In the tissues under the denture base in osteoporotic condition with non-pressure covering, no histopathological changes caused by nonpressure covering were observed except for a transient slight proliferative reaction in the epithelial ridge. The following histopathological changes were observed in the tissues under the denture base in the osteoporotic condition bearing continuous pressure ; (1) bone resorption was induced on the periosteal surface in the osteoporotic-3.4kPa group, and on the pressure side of the endocortical surface in the osteoporotic-1.5 and -3.4kPa group. (2) histopathological changes in the tissues under the denture base in experimental osteoporosis bearing continuous pressure had an intimate relationship with the initial intensity and the time course of continuous pressure, and with the AgNOR counts in mucosal epithelium.

本研究将实验性义齿基托设计为无机械压力覆盖或施加持续压力（0.5、1.5或3.4kPa），对实验性腭裂大鼠磨牙区硬腭进行覆盖。实验性骨质疏松症是通过卵巢切除和低钙饮食喂养引起的。观察了无压力覆盖和持续压力对实验性义齿基托外组织的组织病理学改变及粘膜上皮细胞核仁组成区嗜银蛋白（AgNOR）计数的变化。关于由连续压力诱导的组织学变化，这是相对于连续压力强度的时间过程进行评价的。非加压覆盖义齿基托下组织，除上皮嵴有短暂的轻度增生反应外，无组织病理学改变。结果表明：（1）在持续加压的负压条件下，义齿基托下组织发生了如下组织病理学变化：负压-3.4kPa组骨膜面发生骨吸收，负压-1.5和-3.4kPa组皮质内面压力侧发生骨吸收; (2)实验性骨质疏松持续压力下义齿基托组织的病理变化与持续压力的初始强度、持续压力的时间及粘膜上皮AgNOR计数密切相关。