Analysis of the gene expression of inflammatory mediator in derived cells from synovial fluid from patients with internal derangement.

内部紊乱患者滑液衍生细胞中炎症介质基因表达的分析。

• 批准号: 12671923
• 负责人: FUKUDA Masayuki
• 金额: $ 2.18万
• 依托单位: Akita University
• 依托单位国家: 日本
• 项目类别: Grant-in-Aid for Scientific Research (C)
• 财政年份: 2000
• 资助国家: 日本
• 起止时间: 2000 至 2002
• 项目状态: 已结题
• 来源: https://kaken.nii.ac.jp/grant/KAKENHI-PROJECT-12671923/
• 关键词: temporomandibular joint; internal gerangement; synovium; immunohistochemistry; iNOS; cyclooxygenase; nuclear factor kappa B; 顎関節内障; NO; 炎症性サイトカイン; COX; NF-kappa B; 免疫組織化学; 滑液浮遊細胞; COX-2; NFkappa-B; タンパク分解酵素; PCR

The purpose of this study was to investigate the gene expression of inflammatory mediator in derived cells from synovial fluids, and the immunohistochemical expression and localization of inducible nitric oxide synthase (iNOS), cyclooxygenase-1, -2 (COX-1, COX-2) in synovial tissues from patients with internal derangement (ID) of the temporomandibular joint (TMJ) and patients with condylar fracture of the mandible (FR) as controls. Synovial tissues from patients with ID and from patients with FR were examined for iNOS, COX-1, COX-2 and nuclear factor kappa B (NF-κB) expressions by immunohistochemical staning. Correlation between grade for COX-2 expression and score for synovitis, and clinical findings were also analyzed, iNOS expressed synovial lining layer and blood vessels in the synovium from patients with ID. COX-2 expressed synovial lining layer, infiltrating mononuclear cells, fibroblast-like cells, and blood vessels including CD31 positive endothelial cells in the synovium from patients with ID. However, the expression of COX-1 in synovial lining cells and endothelial cells showed the similar levels both in the diseased specimens and control specimens. There were positive correlations between the expression of the COX-2 and the arthroscopic findings of synovitis (ρ=0.543, p=0.025), and joint pain (ρ=0.566, p=0.02). NF-κB also expressed in the diseased tissues, especially the synovial lining and blood vessels. The results of this study suggested that up-regulation of COX-2 in synovium may play a part in the pathogenesis of synovitis in patients with ID of the TMJ. The gene expression of inflammatory mediator in derived cells from synovial fluid is now investigating.

本研究的目的是研究炎症介质在滑膜液衍生细胞中的基因表达，以及诱导型一氧化氮合酶（iNOS）、环氧合酶-1，2（COX-1，COX-2）的免疫组织化学表达和定位（考克斯-1，考克斯-2）在颞下颌关节（TMJ）内紊乱（ID）和下颌骨髁突骨折（FR）患者滑膜组织中的表达作为对照免疫组化法检测ID和FR患者滑膜组织中iNOS、考克斯-1、考克斯-2和核因子κ B（NF-κB）的表达。分析考克斯-2表达分级与滑膜炎评分及临床表现的相关性，发现ID患者滑膜中iNOS表达滑膜衬里层和血管，ID患者滑膜中考克斯-2表达滑膜衬里层、浸润的单个核细胞、成纤维细胞样细胞和血管，包括CD 31阳性内皮细胞。考克斯-1在滑膜衬里层细胞和内皮细胞的表达水平与正常滑膜组织相似。考克斯-2的表达与滑膜炎（ρ=0.543，p=0.025）和关节疼痛（ρ=0.566，p=0.02）的关节镜检查结果呈正相关。NF-κB在病变组织中也有表达，尤以滑膜和血管为著。本研究结果提示，滑膜组织中考克斯-2的表达上调可能参与了颞下颌关节紊乱病滑膜炎的发病机制。炎症介质的基因表达的衍生细胞从滑液现在正在调查。

项目成果

高橋 哲: "顎関節症の生化学的研究の最前線-顎関節滑液解析の諺断および治療への応用-"東北大学歯学雑誌. 20. 59-74 (2001)

Tetsu Takahashi：“颞下颌关节疾病的生化研究前沿 - 颞下颌关节滑液分析的谚语和治疗应用”东北大学牙科杂志 20. 59-74 (2001)。

Takahashi T: "Current trends in biochemical research of temporomandibular Disorders. Use of synovial analysis for diagnosis and treatment"Tohoku Univ. Dent. J. 20. 59-74 (2001)

Takahashi T：“颞下颌疾病生化研究的当前趋势。利用滑液分析进行诊断和治疗”东北大学。

Takahashi T, Homma H, Nagai H, Seki H, Kondoh T, Yamazaki Y, Fukuda M: "Specific expression of inducible nitric oxide synthase in the synovium of the diseased temporomandibular joint"Oral Surg Oral Med Oral Pathol Oral Radiol Endod. 95. 174-181 (2003)

Takahashi T、Homma H、Nagai H、Seki H、Kondoh T、Yamazaki Y、Fukuda M：“患病颞下颌关节滑膜中诱导型一氧化氮合酶的特异性表达”Oral Surg Oral Med Oral Pathol Oral Radiol Endod。

Takahashi T., Homma H., Nagai H., Seki H., Kondoh T., Yamazaki Y., Fukuda M.: "Specific expression of inducible nitric oxide synthase in the synovium of the diseased temporomandibular joint"Oral Surg.Oral Med.Oral Pathol.Oral Radiol.Endod.. 95. 174-181 (2

Takahashi T.、Homma H.、Nagai H.、Seki H.、Kondoh T.、Yamazaki Y.、Fukuda M.：“患病颞下颌关节滑膜中诱导型一氧化氮合酶的特异性表达”Oral Surg.Oral Med

大貫敬嘉, 福田雅幸, 関 宏, 奈良潤一郎, 船木勝介, 飯野光喜, 高橋 哲: "顎関節クローズドロツク患者の円板後部組織のMRI低信号化の治療前後での比較検討"日本口腔科学会雑誌. 51. 2-7 (2002)

Takayoshi Onuki、Masayuki Fukuda、Hiroshi Seki、Junichiro Nara、Katsusuke Funaki、Mitsuki Iino、Satoshi Takahashi：“颞下颌关节闭合患者治疗前后椎间盘组织MRI低信号的比较研究”日本口腔医学会杂志51。 . 2-7 (2002)