Analysis of the gene expression of inflammatory mediator in derived cells from synovial fluid from patients with internal derangement.

内部紊乱患者滑液衍生细胞中炎症介质基因表达的分析。

• 批准号: 12671923
• 负责人: FUKUDA Masayuki
• 金额: $ 2.18万
• 依托单位: Akita University
• 依托单位国家: 日本
• 项目类别: Grant-in-Aid for Scientific Research (C)
• 财政年份: 2000
• 资助国家: 日本
• 起止时间: 2000 至 2002
• 项目状态: 已结题
• 来源: https://kaken.nii.ac.jp/grant/KAKENHI-PROJECT-12671923/
• 关键词: temporomandibular joint; internal gerangement; synovium; immunohistochemistry; iNOS; cyclooxygenase; nuclear factor kappa B; 顎関節内障; NO; 炎症性サイトカイン; COX; NF-kappa B; 免疫組織化学; 滑液浮遊細胞; COX-2; NFkappa-B; タンパク分解酵素; PCR

The purpose of this study was to investigate the gene expression of inflammatory mediator in derived cells from synovial fluids, and the immunohistochemical expression and localization of inducible nitric oxide synthase (iNOS), cyclooxygenase-1, -2 (COX-1, COX-2) in synovial tissues from patients with internal derangement (ID) of the temporomandibular joint (TMJ) and patients with condylar fracture of the mandible (FR) as controls. Synovial tissues from patients with ID and from patients with FR were examined for iNOS, COX-1, COX-2 and nuclear factor kappa B (NF-κB) expressions by immunohistochemical staning. Correlation between grade for COX-2 expression and score for synovitis, and clinical findings were also analyzed, iNOS expressed synovial lining layer and blood vessels in the synovium from patients with ID. COX-2 expressed synovial lining layer, infiltrating mononuclear cells, fibroblast-like cells, and blood vessels including CD31 positive endothelial cells in the synovium from patients with ID. However, the expression of COX-1 in synovial lining cells and endothelial cells showed the similar levels both in the diseased specimens and control specimens. There were positive correlations between the expression of the COX-2 and the arthroscopic findings of synovitis (ρ=0.543, p=0.025), and joint pain (ρ=0.566, p=0.02). NF-κB also expressed in the diseased tissues, especially the synovial lining and blood vessels. The results of this study suggested that up-regulation of COX-2 in synovium may play a part in the pathogenesis of synovitis in patients with ID of the TMJ. The gene expression of inflammatory mediator in derived cells from synovial fluid is now investigating.

这项研究的目的是研究来自滑液的衍生细胞中炎症介质的基因表达，以及诱导型一氧化氮合酶（INOS）的免疫组织化学表达和定位，环氧酶-1，-2（cox-1，cox-cox-cox酶-1，cox-1，cox-cox）在依从（cox-1，cox-cox）中的依从（ID）患者（cox-1，cox-cox）的内部进化（cox-1，cox-cox），and cox-1，cox-cox酶。下颌骨（FR）作为控件。通过免疫组织化学施加检查了ID和FR患者的滑膜组织和FR患者的iNOS，COX-1，COX-2和核因子KAPPA B（NF-κB）表达。还分析了COX-2表达的等级与滑膜炎的评分之间的相关性，并分析了临床发现，INOS表达的滑膜衬里层和ID患者的滑膜中的血管表达。 COX-2表达滑膜衬里，浸润的单核细胞，成纤维细胞样细胞以及血管，包括ID患者滑膜中的CD31阳性内皮细胞中的血管。但是，在滑膜和内皮细胞中COX-1的表达在差异样本和对照样本中显示出相似的水平。 COX-2的表达与滑膜炎的关节性发现（ρ= 0.543，p = 0.025）与关节疼痛之间存在正相关（ρ= 0.566，p = 0.02）。 NF-κB也在不同组织中表达，尤其是滑膜和血管。这项研究的结果表明，Synovium中COX-2的上调可能在TMJ ID患者的滑膜炎发病机理中起作用。现在正在研究来自滑液衍生细胞中炎症介质的基因表达。

项目成果

高橋 哲: "顎関節症の生化学的研究の最前線-顎関節滑液解析の諺断および治療への応用-"東北大学歯学雑誌. 20. 59-74 (2001)

Tetsu Takahashi：“颞下颌关节疾病的生化研究前沿 - 颞下颌关节滑液分析的谚语和治疗应用”东北大学牙科杂志 20. 59-74 (2001)。

Takahashi T: "Current trends in biochemical research of temporomandibular Disorders. Use of synovial analysis for diagnosis and treatment"Tohoku Univ. Dent. J. 20. 59-74 (2001)

Takahashi T：“颞下颌疾病生化研究的当前趋势。利用滑液分析进行诊断和治疗”东北大学。

Takahashi T, Homma H, Nagai H, Seki H, Kondoh T, Yamazaki Y, Fukuda M: "Specific expression of inducible nitric oxide synthase in the synovium of the diseased temporomandibular joint"Oral Surg Oral Med Oral Pathol Oral Radiol Endod. 95. 174-181 (2003)

Takahashi T、Homma H、Nagai H、Seki H、Kondoh T、Yamazaki Y、Fukuda M：“患病颞下颌关节滑膜中诱导型一氧化氮合酶的特异性表达”Oral Surg Oral Med Oral Pathol Oral Radiol Endod。

Takahashi T., Homma H., Nagai H., Seki H., Kondoh T., Yamazaki Y., Fukuda M.: "Specific expression of inducible nitric oxide synthase in the synovium of the diseased temporomandibular joint"Oral Surg.Oral Med.Oral Pathol.Oral Radiol.Endod.. 95. 174-181 (2

Takahashi T.、Homma H.、Nagai H.、Seki H.、Kondoh T.、Yamazaki Y.、Fukuda M.：“患病颞下颌关节滑膜中诱导型一氧化氮合酶的特异性表达”Oral Surg.Oral Med

大貫敬嘉, 福田雅幸, 関 宏, 奈良潤一郎, 船木勝介, 飯野光喜, 高橋 哲: "顎関節クローズドロック患者の円板後部組織のMRI低信号化の治療前後での比較検討"日本口腔外科学会雑誌. 51・1. 2-7 (2002)

Takayoshi Onuki、Masayuki Fukuda、Hiroshi Seki、Junichiro Nara、Katsusuke Funaki、Mitsuki Iino、Satoshi Takahashi：“闭合性颞下颌关节锁定患者治疗前后后椎间盘组织MRI低信号的比较研究”日本口腔颌面外科学会杂志51・1。