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Epidemiological investigation on the expression of human papillomavirus in oral squamous cell carcinoma and study of its carcinogenesis

口腔鳞状细胞癌中人乳头瘤病毒表达的流行病学调查及其致癌机制研究

基本信息

批准号：
12671939
负责人：
SUGIYAMA Masaru
金额：
$ 1.86万
依托单位：
HIROSHIMA UNIVERSITY
依托单位国家：
日本
项目类别：
Grant-in-Aid for Scientific Research (C)
财政年份：
2000
资助国家：
日本
起止时间：
2000 至 2001
项目状态：
已结题

来源：
https://kaken.nii.ac.jp/grant/KAKENHI-PROJECT-12671939/
关键词：
squamous cell carcinoma human papillomavirus carcinogenesis epidemiological investigation 疲学調査 HPV

项目摘要

1. HPV16 expression in normal oral mucosa (37%) : The positive ratio was 50% in patients over forty years of age, while all the eleven patients under forty showed negative. 2. HPV16 expression in epithelial dysphasia (60%) : The positive ratio was 63% in patients over fifty, while three of four patients were negative under fifty. There was a statistical difference between the positive ratio in this group and that in oral squamous cell carcinoma (OSCC). 3. HPV16 expression in OSCC : (1) Primary lesion (34%) ; The positive ratio was 51% in patients over fifty, while it was 10% in patients under fifty. In the classification based on clinical appearance, the positive ratio (60%) in leukoplakia type was significantly higher than that (19%) in ulcer type but none of the other types. (2) Metastatic lymph node (0%) ; Both primary and metastatic lesions were examined in six cases. All the latter lesions showed negative, although two of six cases showed positive in the former ones. (3) Multicentric carcinoma ; Three of seven multicentric cases and all the three ones suspected of multicentric carcinoma showed positive. The status of HPV infection was not changed, every time it was examined. 4. HPV18, 33 : HPV18 was detected in two cases of multicentric carcinomas, while HPV33 was negative in all cases. 5. HPV16, 18, 33 in cultured OSCC cells : Nine cell lines examined did not show any type of HPV infection. 6. Expression of cyclin D1 was reduced in HPV-show any type of HPV-positive OSCCx than negative ones. In addition, similar tendency was observed in epithelial dysplasias. However, there was no correlation between HPV expression and other cell cycle regulating factors.

1. HPV16在正常口腔黏膜中的表达（37%）：40岁以上的患者中阳性比例为50%，40岁以下的11例患者均为阴性。2. HPV16在上皮性增生中的表达（60%）：在50岁以上的患者中，阳性比例为63%，而在50岁以下的患者中，四分之三的患者呈阴性。本组与口腔鳞状细胞癌（OSCC）的阳性率有统计学差异。3. HPV16在OSCC中的表达：(1)原发病变（34%）；50岁以上患者的阳性比例为51%，而50岁以下患者的阳性比例为10%。在临床表现分型中，白斑型阳性率（60%）明显高于溃疡型阳性率（19%），其他类型均无明显差异。(2)转移性淋巴结（0%）；我们检查了6例原发性和转移性病变。后一种病变均为阴性，前一种病变6例中有2例为阳性。(3)多中心癌；7例多中心癌中3例阳性，3例疑似多中心癌均阳性。每次检查时，HPV感染状况未发生变化。4. HPV18、33:2例多中心癌检出HPV18，所有病例均未检出HPV33。5. HPV16, 18, 33在培养的OSCC细胞中：9个细胞系未显示任何类型的HPV感染。6. 细胞周期蛋白D1的表达在任何类型的hpv阳性OSCCx中均低于阴性。此外，在上皮发育不良中也观察到类似的趋势。然而，HPV的表达与其他细胞周期调节因子没有相关性。