Study on the Mechanism Regulating Cell Differentiation in Salivary Gland Tumor

唾液腺肿瘤细胞分化调控机制研究

• 批准号: 09672050
• 负责人: SUGIYAMA Masaru
• 金额: $ 1.92万
• 依托单位: HIROSHIMA UNIVERSITY
• 依托单位国家: 日本
• 项目类别: Grant-in-Aid for Scientific Research (C)
• 财政年份: 1997
• 资助国家: 日本
• 起止时间: 1997 至 1998
• 项目状态: 已结题
• 来源: https://kaken.nii.ac.jp/grant/KAKENHI-PROJECT-09672050/
• 关键词: SALIVARY GRAND TUMOR; DIFFERENTIATION; INTEGRIN; OSTEOPONTIN; CELL CYCLE REGULATOR; SUPRESSOR GENE; TGF-β

We investigated integrin expression in salivary gland tumors. Α2, α3 and β1 similarly expressed in normal salivary gland (NSG) and pleomorphic adenoma (PA), mucoepidermoid carcinoma (MC), and adenoid cystic carcinoma (ACC). On the other hand, α6 and β4 expressed at basal surface in NSG, PA and MC, but in scattered pattern in ACC. Therefore, we speculated that α6 β4 might have association with invasiveness of ACC rather than cell differentiation.Secondly, we investigated expression of TGF βs and their receptors which stimulate integrin expression and production of extra-cellular matrix. TGF β3 and TGF β RII expressed similarly, but TGF β1 and TGF β2 did not in MC. The former was observed with different intensity in almost all the salivary epithelial cells, in particular epidermoid cells. On the other hand, they were not detected in mucous cells and clear cells. Similar expression pattern was observed in PA and ACC.We immunohistochemically observed localization of osteopontin (OPN) which is ligand for αv integrin using the self-made antibody. OPN expression was observed in all the NSGs and benign and malignant salivary gland tumors tested. OPN was observed in ductal epithelial cells, but not in mucous cells of all the aboved tissues, which suggests the relationship between OPN expression and cell differentiation of salivary gland.Cell cycle regulators, p53, p21 and cycline E, were differently expressed depending on cell component. However, its diversity was supposed to be due to their proliferative ability. The relationship was observed between telomerase activity and malignancy of salivary gland tumor, but not cell differentiation.

我们研究了整合素在唾液腺肿瘤中的表达。Α2、α3和β1在正常唾液腺（NSG）、多形性腺瘤（PA）、粘液表皮样癌（MC）和腺样囊性癌（ACC）中表达相似。α6和β4在NSG、PA和MC细胞的基底表面表达，而在ACC细胞中呈分散表达。因此，我们推测α6 β4可能与ACC侵袭性有关，而不是与细胞分化有关。其次，我们研究了TGF βs及其受体的表达，刺激整合素的表达和细胞外基质的产生。TGF β3和TGF β RII在MC中表达相似，而TGF β1和TGF β2在MC中表达不一致。TGF β3和TGF β2在几乎所有唾液上皮细胞，尤其是表皮样细胞中表达强度不同。另一方面，在粘液细胞和透明细胞中未检测到它们。PA和ACC的表达模式相似。利用自制抗体免疫组化观察αv整合素配体骨桥蛋白（OPN）的定位。所有nsg及唾液腺良恶性肿瘤均有OPN表达。唾液腺导管上皮细胞中均可见到OPN，而上述组织的黏液细胞中均未见OPN表达，提示唾液腺细胞分化与OPN表达有关。细胞周期调节因子p53、p21和cycline E的表达随细胞成分的不同而不同。然而，它的多样性被认为是由于它们的增殖能力。端粒酶活性与唾液腺肿瘤恶性程度有关，但与细胞分化无关。

项目成果

道面仁子: "口腔癌由来細胞株におけるテロメラーゼ構成成分の発現とテロメラーゼ活性"口腔組織培養学会誌. 8・1. 45-46 (1999)

Jinko Domen：“口腔癌来源的细胞系中端粒酶成分和端粒酶活性的表达”口腔组织培养学会杂志8・1（1999）。

DOMEN Tamiko: "Expression of Telomerase Components and Telomerase Activity in Cultured Cells Originated from Oral Cancers"Jpn J Tissue Cult Dnt Res. Vol 8. 45-46 (1999)

DOMEN Tamiko：“源自口腔癌的培养细胞中端粒酶成分和端粒酶活性的表达”Jpn J Tissue Cult Dnt Res。

道面仁子: "口腔癌培養細胞株におけるテロメラーゼRNAの発現とテロメラーゼ活性の検討"口腔組織培養研究会誌. 6・1. 77-78 (1997)

Jinko Domen：“培养的口腔癌细胞系中端粒酶RNA表达和端粒酶活性的表达”口腔组织培养研究会杂志6·1（1997）。

DOMEN Tamiko: "Study on Expression of Telornerase RNA and Telomerase Activity in Cultured Oral Cancer Cells"Jpn J Tissue Cult Dnt Res. Vol 6. 77-78 (1997)

DOMEN Tamiko：“培养口腔癌细胞中端粒酶 RNA 表达和端粒酶活性的研究”Jpn J Tissue Cult Dnt Res。