Inductive effect of blood coagulation factors from endothelial cells by Porphyromonas gingivalis fimbriae

牙龈卟啉单胞菌菌毛对内皮细胞凝血因子的诱导作用

• 批准号: 12672009
• 负责人: HIROSE Kimiharu
• 金额: $ 1.22万
• 依托单位: Health Sciences University of Hokkaido
• 依托单位国家: 日本
• 项目类别: Grant-in-Aid for Scientific Research (C)
• 财政年份: 2000
• 资助国家: 日本
• 起止时间: 2000 至 2001
• 项目状态: 已结题
• 来源: https://kaken.nii.ac.jp/grant/KAKENHI-PROJECT-12672009/
• 关键词: Porphyronmonas gingivalis; Fimbriae; Endothelium; Adhesion; Trypsin; Heart disease; 歯周病原性細菌; 歯周病; レセプター

To Investigate extrinsic blood coagulation pathway, factor VII must be activated to VIIa. Trypsin is known to activate factor VII to VIIa. Here I show that Porphyromonas gingivalis fimbriae stimulate trypsinogen mRNA expression and its protein production in human coronary artery endothelial cells (HCAEC) culture. The monolayer of HCAEC were incubated with or with out various doses of the fimbriae. RT-PCR and western blot analysis were perfomed ti detect and analyze trypsinogen expression in HCAEC. The fimbriae stimulated the expression of trypsinogen mRNA and production of its protein in a fimbriae dose dependent manner. The fimbriae binding to HACEC was specifically evidenced by a binding assay involving radio labeled fimbriae. Additionally, anti-fimbriae antibody (EF3-11) inhibited the fombriae-induces expression of trypsinogen in HCAEC. Finally, inductive effect of trypsinogen production by the fimbriae in HCAEC were inhibited by H7 an inhibitor of PKC. These results demonstrated that fimbriae bind HCAEC, which subsequently lead to stimukate of abnormal blood coagulation factor. These results thus suggest that the possible roles of the periodontopathesic bacteria in abnormal blood coagulation in human heart vascular system.

要研究外源性凝血途径，必须将凝血因子VII激活为凝血因子VIIa。已知胰蛋白酶激活因子VII至VIIa。在这里，我表明，牙龈卟啉单胞菌菌毛刺激胰蛋白酶原mRNA的表达和蛋白质的生产在人冠状动脉内皮细胞（HCAEC）的文化。将HCAEC单层与不同剂量的菌毛一起孵育或不孵育。采用RT-PCR和Western blot方法检测和分析HCAEC中胰蛋白酶原的表达。菌毛以剂量依赖性方式刺激胰蛋白酶原mRNA的表达及其蛋白质的产生。通过涉及放射性标记的菌毛的结合试验，明确证明了菌毛与HACEC的结合。此外，抗菌毛抗体（EF 3 -11）抑制菌毛诱导的HCAEC胰蛋白酶原的表达。PKC抑制剂H7可抑制HCAEC菌毛对胰蛋白酶原的诱导作用。这些结果表明，菌毛结合HCAEC，随后导致异常凝血因子的刺激。这些结果提示牙周炎菌在人心血管系统凝血异常中的作用。

项目成果

Isogai E., Isogai H., Hirose K., Hayashi S., Oguma K.: "In vitro synergy between green tea extact and levofloxacin against enterohemorrhagic Eschericia coli O-157 infection"Current Microbiol.. 42. 248-251 (2001)

Isogai E.、Isogai H.、Hirose K.、Hayashi S.、Oguma K.：“绿茶提取物和左氧氟沙星对抗肠出血性大肠杆菌 O-157 感染的体外协同作用”当前微生物学.. 42. 248-251 (2001)

広瀬公治, 他: "Porphyromonas gingivalis線毛はヒト冠動脈血管内皮細胞に付着する"東日本歯誌. 20. 1-6 (2001)

Koji Hirose 等人：“牙龈卟啉单胞菌菌毛粘附在人冠状动脉内皮细胞上”东日本牙科杂志 20. 1-6 (2001)。

Isogai E., Isogai H., Hirose K.: "In vivo synergy between green tea extract and levofloxacin against enterohemorragic E.coil O-157 infection"Current Microbiology. 42. 248-251 (2001)

Isogai E.、Isogai H.、Hirose K.：“绿茶提取物和左氧氟沙星对抗肠出血性大肠杆菌 O-157 感染的体内协同作用”当前微生物学。

Isogai, E., Hirose, K.et al.: "Therapeutic effect of anti-TNF-α antibody and LVFX in a mouse of enterohemorrhagic E. Coli infection"CIMID. 24. 217-231 (2001)

Isogai，E.，Hirose，K.等人：“抗TNF-α抗体和LVFX对肠出血性大肠杆菌感染的小鼠的治疗效果”CIMID 24. 217-231 (2001)

Hirose K., Isogai E., Ueda I: "Porphyromonas gingivalis fimbriae induce adhesion of monocytic cell line U937 to endothelial cells"Microbiol. Immunol.. 44. 17-22 (2000)

Hirose K.、Isogai E.、Ueda I：“牙龈卟啉单胞菌菌毛诱导单核细胞系 U937 与内皮细胞的粘附”Microbiol。