Search for the New Biological Response Modifiers Targeting on TNFα from the Marine Invertebrates

点击翻译按钮获取中文摘要

Two norcembrane diterpenes, norcembrenolide and sinuleptolide were identified from the Okinawan soft coral (Sinularia sp.), and they demonstrated a potent inhibitory effect on LPS-induced TNFα production by RAW 264 cells.^<1)> A cembrane diterpene, flexibilide (= sinularine) was identified from the Okinawan soft coral (Sinularia flexibilis), and it demonstrated a potent inhibitory effect on TNFα by L929 cells. Five new glycosphingolipids were isolated from the marine sponge (Amphimedon sp.), and they demonstrated a promoting effect on TNFα production by RAW 264 cells.TNFα is well known substance to modulate the angiogenesis. A new anthraquine, bryoanthrathiophene was isolated from the bryozoan (Watersipora subtorquata d' Orbigny, 1852), and it demonstrated the most potent antiangiogenic activity on BAE cells.^<3)> An antiangiogenic purine analog, 1,3-dimethylisoguaninium was identified from the Okinawan marine sponge (Amphimedon paraviridis).^<6)>TNFα is one of the potent death-factors. A new abeo-sterol, orostanal was isolated form the marine sponge (Stelletta hiwasaensis), and orostanal and its synthetic analog induce apoptosis in human acute promyelotic leukemia cells.^<4)>During the term of this project, a new multidrug resistance modulator, irciniasulfonic acid was isolated from the marine sponge (Ircinia sp.).^<5)> A new cytotoxic diterpene, (-)-axinyssene was isolated from the marine sponge (Axinyssa sp.).^<2)>