The effect of the different intensities of exercise training on the co-localization of vasopressin with corticotropin-releasing factor in hypothalamic paraventricular nucleus of rats.

研究了4周和8周不同强度的跑步训练对大鼠下丘脑室旁核（PVN）抗利尿激素（AVP）和促肾上腺皮质激素释放因子（CRF）共定位的影响。6周龄雄性大鼠分为对照组（CTL）和3个训练组，分别以10 m/min （10R）、20 m/min （20R）和30 m/min (30R) 3种不同的跑步机速度进行跑步。运动训练的持续时间、频率和时间分别为30分钟、5天/周、4周和8周。行CRF和AVP双标记免疫荧光染色，计数PVN中同时具有CRF和AVP免疫反应性的神经元数量。在训练4周和8周后第2天称重时，30R组的肾上腺重量显著高于CTL组。在训练4周和8周后的第2天，CTL组和10R、20R、30R 3组均有少量神经元同时具有CRF和AVP免疫反应性。然而，CTL组和3个训练组在神经元数量上无显著差异。如前所述，通过与本研究相同的染色方法，我们观察到肾上腺切除大鼠的PVN神经元中含有CRF和AVP的数量远高于正常大鼠。该结果支持我们在本研究中进行的双标记免疫荧光染色是合适的。由此可见，高强度跑步训练（30m/min速度）不能增强AVP在PVN CRF神经元的共定位，从而导致肾上腺重量增加。

We investigated the effect of the different intensities of treadmill running training for 4 and 8 weeks on the co-localization of vasopressin (AVP) with corticotropin-releasing factor (CRF) in the hypothalamic paraventricular nucleus (PVN) of rats. Six-week-old male rats were divided into control (CTL) and 3 trained groups ran at 3 different treadmill-running speeds that were 10 m/min (10R), 20 m/min (20R) and 30 m/min (30R). The duration, frequency, and periods of exercise training were 30 min, 5 days/weeks, and 4 and 8 weeks, respectively. The double label immunofluorecent staining for CRF and AVP was performed and the number of neurons contained both CRF and AVP immunoreactivity was counted in PVN. The adrenal weights, which were weighed on the 2nd day after 4 and 8 weeks of training, were significantly heavier in 30R than in CTL. A few neurons contained both CRF and AVP immunoreactivity were found in CTL and 3 trained groups, 10R, 20R and 30R on the 2nd day after 4 and 8 weeks of training. However, there were no significant differences in the number of those neurons among CTL and 3 trained groups. As reported previously, by using the same staining that we performed in this study, we observed that the PVN neurons contained both CRF and AVP were much more numerous in adrenalectomized rats than in normal rats. This result supported that the double label immunofluorecent staining we performed in this study was appropriate. To judge from these results, it was suggested that the enhanced co-localization of AVP in the CRF neurons of PVN could not occur with intensive running training (30m/min speed) which caused the increase in adrenal weight.