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The effect of chronic voluntary exercise on induction of Fos expression in the rat brain by nonexercise stressor.

慢性自愿运动对非运动应激源诱导大鼠大脑中 Fos 表达的影响。

基本信息

批准号：
10680033
负责人：
TAN Nobusuke
金额：
$ 2.43万
依托单位：
Yamaguchi University
依托单位国家：
日本
项目类别：
Grant-in-Aid for Scientific Research (C)
财政年份：
1998
资助国家：
日本
起止时间：
1998 至 1999
项目状态：
已结题

来源：
https://kaken.nii.ac.jp/grant/KAKENHI-PROJECT-10680033/
关键词：
voluntary exercise stress Fos opioid blood pressure heart rate brain ラット c-fos

项目摘要

We investigated the effect of voluntary wheel running training for 11-12 wks on induction of Fos expression, which is used as a marker of neuronal activation, in the hypothalamic paraventricular nucleus (PVN) of male rats by restrain stress (experiment 1). We also investigated the effect of running training on cardiovascular response to restrain stress (experiment 1 and 2). In addition, we examined the possibility that activation of opioid systems may be involved in the modified cardiovascular response to stress after running training (experiment 2). In experiment 1, trained (T) rats exhibited significantly (p<0.05) attenuated pressor response to restrain stress compared with control (C) rats. The number of Fos positive cells, which was analyzed quantitatively by immunohistochemistry, in the PVN following restrain stress was lower in T rats than that in C rats, although the difference did not reach statistical significance (p=0.069). In experiment 2, pressor response to restrain stress also tended to be attenuated in T rats. However, intravenous administration of opioid receptor antagonist, naloxone, had no effect on the pressor response to restrain stress in both C and T rats. To judge from these results, it was not clear whether running training, which had an effect in atteuating pressor response to stress, may attenuate neuronal activation in the PVN following nonexercise stressor. In addition, these results did not support the possibility that activation of opioid systems may be involved in the modified cardiovascular response to stress after running training.

本实验观察了11-12周的自由轮跑训练对束缚应激诱导的雄性大鼠下丘脑室旁核（PVN）Fos表达的影响（实验1）。我们还研究了跑步训练对心血管反应的影响，以抑制应激（实验1和2）。此外，我们研究了阿片系统的激活可能参与跑步训练后对应激的心血管反应的改变（实验2）。在实验1中，与对照组（C）大鼠相比，训练组（T）大鼠对束缚应激的升压反应显著减弱（p<0.05）。束缚应激后，T组大鼠PVN中Fos阳性细胞的数量低于C组大鼠，但差异未达到统计学显著性（p=0.069）。在实验2中，T大鼠对束缚应激的升压反应也有减弱的趋势。然而，静脉注射阿片受体拮抗剂纳洛酮对C和T大鼠抑制应激的升压反应均无影响。从这些结果判断，目前尚不清楚跑步训练是否会减弱非运动性应激后PVN的神经元激活，跑步训练可以减弱对应激的升压反应。此外，这些结果并不支持阿片系统的激活可能参与跑步训练后对应激的心血管反应的改变。