Age related functional and morphological alterations in rat diaphragm and phrenic motoneuron

大鼠膈肌和膈运动神经元与年龄相关的功能和形态变化

• 批准号: 12680035
• 负责人: MIYATA Hirofumi
• 金额: $ 2.11万
• 依托单位: Yamaguchi University
• 依托单位国家: 日本
• 项目类别: Grant-in-Aid for Scientific Research (C)
• 财政年份: 2000
• 资助国家: 日本
• 起止时间: 2000 至 2002
• 项目状态: 已结题
• 来源: https://kaken.nii.ac.jp/grant/KAKENHI-PROJECT-12680035/
• 关键词: Rat; Phrenic motoneuron; Diaphragm; Muscle fiber; Rheobase; Input resistance; Specific force; Contraction speed

No significant differences were found in all parameters in recruited phrenic motoneuron, while the rheobase of the non-recruited motoneurons was significantly lower in the 2-year group than the other two groups. The mean interval of action potentials of the recruited motoneurons was longer in the 1- and 2-year groups than the 10-week group, and the firing pattern in 2-year group was considerably irregular. Although there were no significant differences in the twitch tension among the groups, tetanus tensions by high frequency stimulation (20-100Hz) were significantly lower in the older group. The time to peak tension and half relaxation time were significantly longer in aged diaphragm muscles. The histochemical profiles of aged diaphragm muscle showed a smaller area of FG fibers and higher percentage of SO fibers.

招募的膈运动神经元的所有参数均无显著差异，而未招募的运动神经元的基础值在2年组显著低于其他两组。1、2年龄运动神经元的平均动作电位间期均长于10周龄组，2年龄组的放电模式明显不规则。虽然各组间的抽动张力无显著差异，但高频刺激(20-100 Hz)引起的破伤风张力在老年组显著降低。老年横隔肌的张力达峰时间和半松弛时间明显延长。组织化学结果显示，老年横隔肌FG纤维面积较小，SO纤维所占比例较高。

项目成果

Miyata H., Wada N.: "Denervation causes changes in electiophysiological properties in rat phrenic motoneurons"Neurosci. Lett.. 310. 149-152 (2001)

Miyata H.，Wada N.：“去神经支配导致大鼠膈运动神经元电生理特性的变化”Neurosci。

Miyata H., Wada N.: "Denervation causes changes in electrophysiological properties in rat phrenic motoneurons"Neurosci. Lett.. 310. 149-152 (2001)

Miyata H.，Wada N.：“去神经支配导致大鼠膈运动神经元电生理特性的变化”Neurosci。

Miyatja H., Wada N.: "Electrophysiological and metabolical properties of compensatory activated phrenic motoneuron in rats."Med. & Sci. in Sports & Exer.. 31(5). s216 (2002)

Miyatja H.，Wada N.：“大鼠代偿性激活膈运动神经元的电生理学和代谢特性。”医学。

Miyata H., Yamano S., Shyono E.: "Age-related functional changes in the rat diaphragm and phrenic motoneuron"Med. & Sci. in Sports & Exer.. 32(5)in press. (2003)

Miyata H.、Yamano S.、Shyono E.：“大鼠膈肌和膈运动神经元的年龄相关功能变化”Med。

Miyata H, Yamano S, Shyono E: "Age-related functional changes in the rat diaphragm and phrenic motoneuron"Med. & Sci in Sports & Exer.. 32(5)(In press). (2003)

Miyata H、Yamano S、Shyono E：“大鼠膈肌和膈运动神经元的年龄相关功能变化”Med。