Age-related Change in Endplate morphology and its regulatory factors in rat muscles

大鼠肌肉终板形态的年龄相关变化及其调控因素

• 批准号: 21300251
• 负责人: MIYATA Hirofumi
• 金额: $ 8.99万
• 依托单位: Yamaguchi University
• 依托单位国家: 日本
• 项目类别: Grant-in-Aid for Scientific Research (B)
• 财政年份: 2009
• 资助国家: 日本
• 起止时间: 2009-04-01 至 2014-03-31
• 项目状态: 已结题
• 来源: https://kaken.nii.ac.jp/grant/KAKENHI-PROJECT-21300251/
• 关键词: エンドプレート; シナプス核; Musk; 加齢変化; 加齢; MuSK; Sｈｐ２; 神経筋接合部; サテライトセル; MuSK mRNA; 横隔膜; 脱神経; musk

We examined the number of endplate nuclei, morphological properties of the motor endplate, and the expression level of muscle-specific kinase (MuSK) and tyrosine phosphatase with two SH2 domains (Shp2) in rat muscles with aging.During the developmental period (1 to 25 weeks), the muscle fiber diameter, endplate volume and surface area, and number of endplate nuclei were significantly increased in both DIA and STM muscles. The level of MuSK mRNA expression was decreased by 60% during the developmental period and kept constant values to 130 weeks after birth. While, the level of SHP2 mRNA expression kept constant value during the developmental period and increased to 130 weeks. There were significantly higher levels of MuSK and SHP2 mRNA expression in DIA than those in STM muscles. These results suggested that enlargement of endplate is mainly regulated by MuSK expression during postnatal development, and that fragmentation of endplate is mainly regulated by SHP2 expression with aging.

结果表明，在发育过程中（1 ~ 25周），DIA和STM肌纤维直径、终板体积、终板面积和终板核数均显著增加，终板核数、终板面积和终板核数均显著增加。MuSK mRNA的表达水平在发育过程中下降60%，并在出生后130周内保持恒定值。而SHP 2 mRNA的表达水平在整个发育过程中基本保持不变，到130周时逐渐增加。DIA组MuSK和SHP 2 mRNA表达水平显著高于STM组。这些结果表明，在出生后发育过程中，终板的扩大主要受MuSK表达的调控，而终板的碎裂主要受SHP 2表达的调控。

项目成果

ラット発育期におけるエンドプレート核数およびMuSK 発現の変化

大鼠发育过程中终板核数量和MuSK表达的变化

• 发表时间: 2012
• 作者: 宮田浩文;田中麻衣子;福場良之
• 通讯作者: 福場良之

ラット横隔膜のシナプス核数に及ぼす加齢・脱神経の影響

衰老和去神经对大鼠膈肌突触核数量的影响

• 发表时间: 2009
• 作者: 桜井良太;藤原佳典;齋藤京子;安永正史;金美芝;小川貴志子;鈴木克彦;西川武志;渡辺修一郎;新開省二;山口茜
• 通讯作者: 山口茜

ラットエンドプレートにおけるシナプス核ドメインとMuSK 発現の加齢変化

大鼠终板突触核结构域和 MuSK 表达的年龄相关变化

• 发表时间: 2011
• 作者: 青山友子;浅香明子;金子香織;石島寿道;河野寛;坂本静男;田畑泉;樋口満;宮田浩文
• 通讯作者: 宮田浩文

Muscle Fiber Population and Biochemical Properties of Whole Body Muscles in Thoroughbred Horses

• DOI: 10.1002/ar.20961
• 发表时间: 2009-10-01
• 期刊: ANATOMICAL RECORD-ADVANCES IN INTEGRATIVE ANATOMY AND EVOLUTIONARY BIOLOGY
• 影响因子: 2
• 作者: Kawai, Minako;Minami, Yoshio;Miyata, Hirofumi
• 通讯作者: Miyata, Hirofumi

Developmental Change in Domain Size of Endplate Nucleus in the Rat Diaphragm

大鼠膈肌终板核域大小的发育变化

• 发表时间: 2012
• 作者: Miyata H;Tanaka M;Yamaguchi A
• 通讯作者: Yamaguchi A