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Molecular analysis of early body plan formation in lancelet embryos

文昌鱼胚胎早期身体结构形成的分子分析

基本信息

批准号：
12680719
负责人：
YASUI Kinya
金额：
$ 1.92万
依托单位：
Kumamoto University (2001)Kagoshima University (2000)
依托单位国家：
日本
项目类别：
Grant-in-Aid for Scientific Research (C)
财政年份：
2000
资助国家：
日本
起止时间：
2000 至 2001
项目状态：
已结题

来源：
https://kaken.nii.ac.jp/grant/KAKENHI-PROJECT-12680719/
关键词：
amphioxus chordate maternal factor embryonic axis cleavage microtubule LiCl body plan amphioxus larval body plan Wnt peripheral nerve asymmetry coolom cephalochordate BMP

项目摘要

Expression patterns of wnt7, wnt8, bmp2/4, brachyury, hnf3-β were studied from the gastrula to neurula stage in Chinese lancelets. Of them, bmp2/4 shows a specific expression pattern compared with that in vertebrates. Since regions where bmp2/4 is expressed in lancelets correspond to the region where the genes are inhibited their expression in vertebrates, this gene may play an basic function in anterior specific body formation in this animal. The other genes are similar to their vertebrate cognates in their expression pattern. The spatial expression pattern of these genes suggests that the early lancelet embryo has two distinct regions anteroposteriorly, each of which develops under different molecular mechanisms. The two distinct developmental domains may be related to the tripartite coelomic system that has been proposed as a basic body plan of deuterostomes.To investigate function of maternal molecules in patterning expression of earliest zygotic genes, we screened maternal molecul … More es, β-catenin protein, nodal, Tbrain/eomesodermin (Tbr/eomes), FGF receptor mRNA have been identified. Although spatial pattern of their molecules show their own specificity, they first move to or are accumulated around the animal pole before or during the onset of the first cleavage, and then move or spread vegetally. Some of them become distributed asymmetrically between the cleaving blastomeres. Distribution of microtubules in eggs and embryos during the first cleavage shows a similar pattern, suggesting mechanical relationships between maternal factors and microtubules. The fact that LiCl treatment affects distribution of β-catenin, Tbr/eomes and microtubules supports a role of microtubules in distribution of the maternal factors after fertilization. Dorsal specific genes, otx, goosecoid and lim3, modify their expression pattern when treated with LiCl as in vertebrates. However, treated embryos still establish dorsoventral and left-right polarities. These results suggest that the lancelet embryo utilizes a similar set of molecules during the initial development but in different way from vertebrates, the distribution pattern of maternal molecules suggests that embryonic polarity is established during the first cleavage. Less

研究了中国文昌鱼从原肠胚到神经胚阶段的wnt7、wnt8、bmp2/4、brachyury、hnf3-β的表达模式。其中，bmp2/4与脊椎动物相比表现出特定的表达模式。由于bmp2/4在文昌鱼中表达的区域对应于该基因在脊椎动物中表达受到抑制的区域，因此该基因可能在该动物的前部特定身体形成中发挥基本功能。其他基因的表达模式与脊椎动物同源基因相似。这些基因的空间表达模式表明，早期文昌鱼胚胎前后有两个不同的区域，每个区域在不同的分子机制下发育。这两个不同的发育域可能与三方体腔系统有关，该系统已被提议为后口动物的基本身体计划。为了研究母体分子在最早合子基因表达模式中的功能，我们筛选了母体分子… 更多 es、β-连环蛋白、节点、Tbrain/eomesodermin (Tbr/eomes)、FGF 受体 mRNA 已被鉴定。尽管它们的分子的空间模式显示出自己的特异性，但它们首先在第一次卵裂开始之前或期间移动到动物极或聚集在动物极周围，然后以植物方式移动或传播。其中一些在分裂卵裂球之间不对称分布。第一次卵裂期间卵子和胚胎中微管的分布显示出类似的模式，表明母体因素与微管之间存在机械关系。 LiCl 处理影响 β-连环蛋白、Tbr/eome 和微管的分布这一事实支持微管在受精后母体因子分布中的作用。与脊椎动物一样，背部特异性基因 otx、goosecoid 和 lim3 在用 LiCl 处理时会改变其表达模式。然而，经过处理的胚胎仍然建立背腹和左右极性。这些结果表明，文昌鱼胚胎在初始发育过程中利用了一组相似的分子，但与脊椎动物不同，母体分子的分布模式表明胚胎极性是在第一次卵裂期间建立的。较少的