Role of Ca channels and transmitters in cortical oscillatory activity

Ca 通道和递质在皮质振荡活动中的作用

• 批准号: 12680746
• 负责人: MURAKOSHI Takayuki
• 金额: $ 2.37万
• 依托单位: Tokyo Medical and Dental University
• 依托单位国家: 日本
• 项目类别: Grant-in-Aid for Scientific Research (C)
• 财政年份: 2000
• 资助国家: 日本
• 起止时间: 2000 至 2001
• 项目状态: 已结题
• 来源: https://kaken.nii.ac.jp/grant/KAKENHI-PROJECT-12680746/
• 关键词: oscillatory neural activity; synchronous neural activity; consciousness; cerebral cortex; thalamus; local neural circuit; calcium channel; brain slice; 同期神経活動

The oscillatory and/or synchronous neuronal activities are considered to play important roles in maintenance of the consciousness and in performance of higher CNS functions. In order to elucidate the mechanisms controlling the oscillatory activity in terms of io* channels and local network, spontaneous or stimulus-evoked oscillatory excitation wa* recorded electrophysiologically from the slice preparation which reserved thalamo-cortica* neural connections. Optical recordings of the membrane potential using a voltage-sensitive dye revealed that the thalamus and the primary somatosensory area (the barrel cortex) are connected within the slice plane. Using whole-cell patch-clamp methods, 10-40 Hz fluctuation of the membrane voltage/current for 1-2 s was observed in response to tetani* stimulation of the internal capsule which supplies inputs to the cortex. However, this oscillatory activity was not affected by application of Ni (a blocker of the T-type and R-type voltage-dependent calcium channels: VDCC) or bicuculline (a GABAA receptor antagonist), suggesting that the activity is neither derived from the network interactions an* nor strongly dependent on the above-mentioned VDCCs. Therefore, strategy was slightl* modified to pursue the network oscillations in the hippocampal CA3 region. The agent which increase neuronal excitability such as carbachol induced an increase in the neuronal activities of >10 Hz and a decrease in those of lower frequency. This action was dependent on the temperature of the perfusate and on the age of the animals from which the preparations were obtained. Propofol, an intravenous anesthetic, reduced the action c* carbachol to induce oscillation.

振荡和/或同步神经元活动被认为在维持意识和执行更高的CNS功能中起重要作用。为了阐明控制振荡活动的离子通道和局部网络的机制，我们在保留丘脑-皮质神经连接的脑片上记录了自发或刺激诱发的振荡兴奋。使用电压敏感染料的膜电位的光学记录显示，丘脑和初级躯体感觉区（桶皮质）内的切片平面连接。用全细胞膜片钳方法，观察到10-40 Hz的膜电压/电流波动1-2 s响应于提供输入到皮层的内囊的破伤风刺激。然而，这种振荡活性不受Ni（T型和R型电压依赖性钙通道的阻断剂：VDCC）或荷包牡丹碱（GABAA受体拮抗剂）的影响，表明该活性既不是来自网络相互作用，也不是强烈依赖于上述VDCC。因此，对策略进行了轻微修改，以追踪海马CA 3区的网络振荡。增加神经元兴奋性的试剂如卡巴胆碱诱导>10 Hz的神经元活动增加和较低频率的神经元活动减少。该作用取决于灌注液的温度和获得制剂的动物的年龄。异丙酚，静脉麻醉剂，减少行动c* 卡巴胆碱诱导振荡。

项目成果

Kubota,M.,Murakoshi,T. et al.: "Intact LTP and fear memory but impaired spatial memory in mice lacking Ca_v2.3 (α_<1E>) channel"Biochem.Biophys.Res.Comm.. (in press). (2001)

Kubota, M.、Murakoshi, T. 等人：“缺乏 Ca_v2.3 (α_<1E>) 通道的小鼠的 LTP 和恐惧记忆完整，但空间记忆受损”Biochem.Biophys.Res.Comm..（出版中） (2001)

Sakata, Y. et al.: "Analysis of Ca^<2+>currents in spermatocytes from mice lacking Ca_v2.3 (α1_E) Ca^<2+>cannel"Biochem. Biophys. Res. Comm.. 288. 1032-1036 (2001)

Sakata，Y.等人：“缺乏Ca_v2.3 (α1_E) Ca ^ 2+ 通道的小鼠精母细胞中Ca ^ 2+ 电流的分析”Biochem.Res. 288。 1036 (2001)

Toriyama, H., et al.: "Role of Ca_v2.3 (α_<1E>) Ca2+ channel in ischemic neuronal injury"Neuroreport. 13. 261-265 (2002)

Toriyama, H., et al.：“Ca_v2.3 (α_<1E>) Ca2+ 通道在缺血性神经损伤中的作用”Neuroreport. 13. 261-265 (2002)

Sakata,Y., et al.: "Analysis of Ca2+ corrents in spermatocytes from mice lacking Ca_v2.3 (α_<1E>) Ca2+ channel"Biochem. Biophys. Res. Comm.. 288. 1032-1036 (2001)

Sakata，Y.，等人：“缺乏Ca_v2.3(α_<1E>)Ca2+通道的小鼠精母细胞中的Ca2+电流的分析”Biochem.Res.288.1032-1036(2001)。

<Murakoshi. T.>_____________-, et al: "Multiple G-protein-coupled receptors mediate presynaptic inhibition at single excitatory synapses in the rat visual cortex"Neuroscience Letters. 309. 117-120 (2001)

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