Research on behavior analysis of microbubbles for ultrasound contrast agent and optimum ultrasound diagnostic system

超声造影剂微泡行为分析及优化超声诊断系统研究

• 批准号: 12680849
• 负责人: TAKEUCHI Shinichi
• 金额: $ 0.7万
• 依托单位: Toin University of Yokohama
• 依托单位国家: 日本
• 项目类别: Grant-in-Aid for Scientific Research (C)
• 财政年份: 2000
• 资助国家: 日本
• 起止时间: 2000 至 2001
• 项目状态: 已结题
• 来源: https://kaken.nii.ac.jp/grant/KAKENHI-PROJECT-12680849/
• 关键词: nonlinear response; ultrasound contrast agents; microbubble; Langevine type ultrasound transducer; ultrasound transducers with double peak type frequency characteristics; harmonic imaging; subharmonic imaging; ultrasound diagnostic system; コティング膜

The purposes of this research are the development of experimental and analyzing system of response of microbubble ultrasound contrast agents to ultrasound exposure and clarification of the relation between the structure of microbubbles and response of microbubbles against ultrasound exposure including nonlinear response. Furthermore, we aimed at the development of ultrasound diagnostic system using nonlinear behavior of microbubbles and ultrasound diagnostic system optimizing the performance of microbubbles for ultrasound contrast agents.For these purpose, it was found that the shell elasticity parameter S_p was 0.3 N/m and the shell friction parameter S_f was 0.07 μNs/m by curve fitting between experimental and theoretical results with numerical calculation for the hand made surfactant coated microbubbles manufactured by bubbling in the mixed solution of sodium stearate, sodium palmitate and saponine and adding calcium chloride.It was found by nonlinear differential equation consideri … More ng the effect of coating membrane of microbubbles and the effect of compressibility of the liquid surrounding microbubbles proposed by us that the optimum microbubble for harmonic imaging has the small shell elasticity parameter and large shell friction parameter.The microbubble behavior observation systems by forming standing wave acoustic field in water from the bottom of water tank with stainless vibrating disc equipped with the Langevine type ultrasound transducer and by holding microbubbles at the position corresponding to the loop of standing wave acoustic field in water were manufactured.We can mention by these results that our hand made surfactant coated microbubbles were appropriate to harmonic imaging comparing with commercial microbubble ultrasound contrast agents.The life time of this microbubble was estimated with the change of brightness in the B-mode image of ultrasound diagnostic equipment. Furthermore, it was found that the ultrasound exposure condition appropriate to harmonic imaging with this microbubble were (1) to coincide between the ultrasound exposure frequency and the resonant frequency of microbubble and (2) to coincide between the ultrasound exposure frequency and the half of resonant frequency of microbubble.The ultrasound transducers with double peak type frequency characteristics for harmonic imaging and those for subharmonic imaging were proposed and developed and the availability of these trially manufactured ultrasound transducers were confirmed on the basis of these results. As the fundamental ultrasound component can be radiated and the second harmonic component or subharmonic component can be detected effectively by these ultrasound transducers with double peak type frequency characteristics, these ultrasound transducers can be thought as the key device for optimum ultrasound diagnostic system using nonlinear behavior of microbubbles. Less

本研究的目的是建立微气泡超声造影剂对超声辐射响应的实验和分析系统，阐明微气泡结构与微气泡对超声辐射响应的关系，包括非线性响应。针对超声造影剂中微泡的非线性行为和微泡性能优化的超声诊断系统的开发，通过对硬脂酸钠、棕榈酸钠和皂苷混合溶液中鼓泡并添加氯化钙制成的手工型表面活性剂涂层微泡的数值计算和实验结果的曲线拟合，得出微泡的壳体弹性参数S_p为0.3N/m，壳体摩擦参数S_f为0.07μNS/m。通过考虑…的非线性微分方程式，得到表面活性剂涂层微泡的壳体弹性系数为0.3N/m，壳体摩擦系数为0.07 nS/m进一步考虑了微泡包膜效应和微泡周围液体的可压缩性效应，提出了谐波成像的最佳微泡具有较小的壳层弹性参数和较大的壳层摩擦系数。利用装有朗之万型超声换能器的不锈钢振动盘在水箱底部形成水中驻波声场，并将微泡夹在水中与水中驻波声场环相对应的位置，研制了微气泡行为观测系统。实验结果表明，与市售的微气泡超声造影剂相比，自制的表面活性剂涂层微气泡适合于谐波成像根据超声诊断仪B超图像中亮度的变化来估算微泡的寿命。在此基础上，提出了适合微泡谐波成像的超声曝光条件：(1)超声曝光频率与微泡的共振频率重合；(2)超声曝光频率与微泡共振频率的一半重合。提出并研制了具有双峰型频率特性的谐波成像和亚谐成像超声换能器，并在此基础上验证了这些试制的换能器的可行性。由于这些具有双峰型频率特性的超声换能器可以辐射基波分量，并能有效地检测二次谐波分量或次谐波分量，因此这些换能器可以被认为是利用微泡的非线性行为来优化超声诊断系统的关键器件。较少

项目成果

Shinichi Takeuchi: "Nonlinear response of microbubbles coated with surfactant membrane developed as ultrasound contrast agent -experimental study and numerical calculations-"Colloids and Surfaces B : Biointerfaces. (2002)

Shinichi Takeuchi：“作为超声造影剂开发的涂有表面活性剂膜的微泡的非线性响应 - 实验研究和数值计算 -”胶体和表面 B：生物界面。

Shinichi Takeuchi: "Development of ultrasound transducer with double peak type frequency characteristics for harmonic imaging ultrasound diagnostic equipment"Proceedings of IEEE Ultrasonic Symposium in Atlanta. 1151-1155 (2002)

Shinichi Takeuchi：“开发用于谐波成像超声诊断设备的具有双峰型频率特性的超声换能器”亚特兰大 IEEE 超声研讨会论文集。

Shinichi Takeuchi: "Detection of harmonic ultrasound scattered from microbubbles with ultrasound transducer for harmonic imaging having double peak type frequency characteristics"Colloids and Surfaces B : Biointerfaces. (2002)

Shinichi Takeuchi：“用超声波传感器检测从微泡散射的谐波超声波，用于具有双峰型频率特性的谐波成像”胶体和表面 B：生物界面。

Takeuchi,Shinichi, Soetanto,Kawan, Sato,Toshio and Kawashima,Norimichi: "Optimum ultrasounc exposure condition for excitation of nonlinear behavior of microbubbles coated with surfactant"Acoustical Science and Technology. Vol. 22, no. 5. 364-366 (2001)

Takeuchi、Shinichi、Soetanto、Kawan、Sato、Toshio 和 Kawashima、Norimichi：“激发表面活性剂包覆微泡非线性行为的最佳超声暴露条件”声学科学与技术。

Takeuchi,Shinichi, Sato,Toshio and Kawashima,Norimichi: "Nonlinear response of microbubbles coated with surfactant membrane developed as ultrasound contrast agent -Experimantal study and numerical calculations-"Colloids and Surfaces B: Biointerfaces. Vol.

Takeuchi、Shinichi、Sato、Toshio 和 Kawashima、Norimichi：“作为超声造影剂开发的表面活性剂膜涂覆的微泡的非线性响应 - 实验研究和数值计算 -”胶体和表面 B：生物界面。