Molecular devices by functionalization of modified DNA

通过修饰 DNA 功能化的分子装置

• 批准号: 13305058
• 负责人: MAJIMA Tetsuro
• 金额: $ 35.53万
• 依托单位: Osaka University
• 依托单位国家: 日本
• 项目类别: Grant-in-Aid for Scientific Research (A)
• 财政年份: 2001
• 资助国家: 日本
• 起止时间: 2001 至 2004
• 项目状态: 已结题
• 来源: https://kaken.nii.ac.jp/grant/KAKENHI-PROJECT-13305058/
• 关键词: DNA; DNA molecular wire; Modified DNA; Functionalization of DNA; Molecular device; Nucleic acid; Hole transfer; Laser photolysis; パルスラジオリシス

In this research, it is aimed that various functionalities are given to modification DNA by using DNA modified covalently with various functional chromophores, controlling the functionalities can be established, and the possibility of modified DNA as molecular devices in the living body system is clarified. Especially, in order to clarify the hole transfer in DNA with respect to the construction of DNA molecular wire, photofunctions of modified DNA having photofunctional chromophores has been studied in the nano meter scale depending on the nuclear base sequences. Not only one chromophore but also several chromophores are modified on one DNA in order to clarify the charge separation, hole migration, and charge recombinataion in the nano meter scale and in the nano-pico second time scale. Technology that accurately operates functional molecule by means of molecular assembly is necessary to the nanotechnology of a bottom-up type, and it is necessary to establish the method of molecular assembly according to artificial information. DNA is the most powerful molecule where an accurate molecular assembly is achieved with many kinds of molecules and has the advantage that a variety of functional molecules can be introduced by the chemical modification in DNA. Therefore, DNA unit with the branch dividing structure was synthesized to construct a regular and rigid DNA structures based on self-assembly, and we succeeded to construct DNA nano structure to use this constructing a bridge DNA. In order to establish molecular devices by functionalization of modified DNA more precisely, to achieve the multifunction like the chlorophyll, photofunction, and complex functions is very important. It is also necessary to clarify the method of construction of modified DNA with a high performance and molecular device as a biomolecular function, and to develop the possibility of modified DNA as molecular devices in the living body system.

In this research, it is aimed that various functionalities are given to modification DNA by using DNA modified covalently with various functional chromophores, controlling the functionalities can be established, and the possibility of modified DNA as molecular devices in the living body system is clarified. Especially, in order to clarify the hole transfer in DNA with respect to the construction of DNA molecular wire, photofunctions of modified DNA having photofunctional chromophores has been studied in the nano meter scale depending on the nuclear base sequences. Not only one chromophore but also several chromophores are modified on one DNA in order to clarify the charge separation, hole migration, and charge recombinataion in the nano meter scale and in the nano-pico second time scale. Technology that accurately operates functional molecule by means of molecular assembly is necessary to the nanotechnology of a bottom-up type, and it is necessary to establish the method of molecular assembly according to artificial information. DNA is the most powerful molecule where an accurate molecular assembly is achieved with many kinds of molecules and has the advantage that a variety of functional molecules can be introduced by the chemical modification in DNA. Therefore, DNA unit with the branch dividing structure was synthesized to construct a regular and rigid DNA structures based on self-assembly, and we succeeded to construct DNA nano structure to use this constructing a bridge DNA. In order to establish molecular devices by functionalization of modified DNA more precisely, to achieve the multifunction like the chlorophyll, photofunction, and complex functions is very important. It is also necessary to clarify the method of construction of modified DNA with a high performance and molecular device as a biomolecular function, and to develop the possibility of modified DNA as molecular devices in the living body system.

项目成果

Formation and Decay of Pyrene Radical Cation and Pyrene Dimer Radical Cation in the Absence and Presence of Cyclodexitrins during Resonant Two-Photon Ionization of Pyrene and Sodium 1-Pyrene Sulfonate

芘和 1-芘磺酸钠共振双光子电离过程中环糊精不存在和存在下芘自由基阳离子和芘二聚物自由基阳离子的形成和衰变

• 发表时间: 2004
• 期刊: Phys.Chem.Chem.Phys. 6
• 作者: 立川貴士;真嶋哲朗;T. Tachikawa;K. Naito;T. Tachikawa;M. Sakamoto;X. Cai;M. Sakamoto;X. Cai;M. Sakamoto;Masanori Sakamoto;Kiyohiko Kawai;Mamoru Fujitsuka;Michihiro Hara;Takashi Tachikawa;Yosuke Oseki;Shingo Samori;Takashi Tachikawa;Yosuke Oseki;Kazuya Naito;Masanori Sakamoto;Mamoru Fujitsuka;Masanori Sakamoto;Xichen Cai;Masanori Sakamoto;Michihiro Hara;Takumi Kimura;Shinro Yasui;Shingo Samori;Michihiro Hara;Shingo Samori;Masayuki Endo;Koji Nakayama;Kiyohiko Kawai;Masayuki Endo;Masayuki Endo;Kimio Akiyama;Masayuki Endo;Takashi Tachikawa;Tadao Takada;Xichen Cai;Kiyohiko Kawai;Takahiro Kawai;Xichen Cai;Kiyohiko Kawai;Takashi Tachikawa;Kiyohiko Kawai;Kiyohiko Kawai;Mamoru Fujitsuka;Michihiro Hara;Takashi Tachikawa;Masanori Sakamoto;Shingo Samori;Kiyohiko Kawai;Kiyohiko Kawai;Tadao Takada;Masanori Sakamoto;Mamoru Fujitsuka;T. Tachikawa;T. Tachikawa;T. Tachikawa;T. Tachikawa;T. Tachikawa;T. Tachikawa;s. Tojo;S. Tojo;T. Tachikawa;T. Tachikawa;X. Cai;M. Sakamoto;X. Cai;M. Sakamoto;T. Tachikawa;T. Tachikawa;T. Tachikawa;T. Tachikawa;T. Tachikawa;S. Tojo;S. Tojo;T. Tachikawa;X. Cai;M. Sakamoto;M. Sakamoto;Tadao Takada;Tadao Takada;Xichen Cai;Masanori Sakamoto;Kiyohiko Kawai;Xichen Cai;Masanori Sakamoto;Xichen Cai;Masanori Sakamoto;Tadao Takada;Takashi Tachikawa;Takashi Tachikawa;Mamoru Fujitsuka;Kiyohiko Kawai;Yosuke Oseki;Takashi Tachikawa;Takashi Tachikawa;Takashi Tachikawa;Takashi Tachikawa;Takumi Kimura;Masayuki Endo;Kiyohiko Kawai;Masayuki Endo;Takashi Tachikawa;Mamoru Fujitsuka;Takumi Kimura;Masayuki Endo;Takumi Kimura;Koji Nakayama;Sachiko Tojo;Michihiro Hara;Takashi Tachikawa;Michihiro Hara;Michihiro Hara;Sachiko Tojo;Xichen Cai;Michihiro Hara
• 通讯作者: Michihiro Hara

Masayuki Endo, Tetsuro Majima: "Control of A Double Helix Assembly by Use of Crosslinked Oligonucleotides"J.Am.Chem.Soc.. 125・45. 13654-13655 (2003)

Masayuki Endo、Tetsuro Majima：“使用交联寡核苷酸控制双螺旋组装”J.Am.Chem.Soc. 125・45（2003）。

Michihiro Hara: "Inhibition of one-electron oxidation of 1-pyrenesulfonate included in cyclodextrin by sulfate radical anion"Chem.Phys.Lett.. (in press). (2004)

Michihiro Hara：“硫酸根阴离子对环糊精中包含的 1-芘磺酸盐的单电子氧化的抑制”Chem.Phys.Lett..（出版中）。

Anomalous emitting property of azaxanthone ketylradical in the excited state

氮杂蒽酮酮自由基激发态的反常发光特性

• 发表时间: 2005
• 期刊: J. Am. Chem、Soc. (Commun. ) 127(11)
• 作者: M;Sakamoto
• 通讯作者: Sakamoto

Lifetime Regulation of Charge-Separated Sate in DNA by Modulating the Oxidation Potential of Guanine in DNA through Hydrogen Bonding

通过氢键调节 DNA 中鸟嘌呤的氧化电位来调节 DNA 中电荷分离状态的寿命

• 发表时间: 2004
• 期刊: J.Am.Chem.Soc. 126(40)
• 作者: 立川貴士;真嶋哲朗;T. Tachikawa;K. Naito;T. Tachikawa;M. Sakamoto;X. Cai;M. Sakamoto;X. Cai;M. Sakamoto;Masanori Sakamoto;Kiyohiko Kawai;Mamoru Fujitsuka;Michihiro Hara;Takashi Tachikawa;Yosuke Oseki;Shingo Samori;Takashi Tachikawa;Yosuke Oseki;Kazuya Naito;Masanori Sakamoto;Mamoru Fujitsuka;Masanori Sakamoto;Xichen Cai;Masanori Sakamoto;Michihiro Hara;Takumi Kimura;Shinro Yasui;Shingo Samori;Michihiro Hara;Shingo Samori;Masayuki Endo;Koji Nakayama;Kiyohiko Kawai;Masayuki Endo;Masayuki Endo;Kimio Akiyama;Masayuki Endo;Takashi Tachikawa;Tadao Takada;Xichen Cai;Kiyohiko Kawai;Takahiro Kawai;Xichen Cai;Kiyohiko Kawai;Takashi Tachikawa;Kiyohiko Kawai;Kiyohiko Kawai;Mamoru Fujitsuka;Michihiro Hara;Takashi Tachikawa;Masanori Sakamoto;Shingo Samori;Kiyohiko Kawai;Kiyohiko Kawai;Tadao Takada;Masanori Sakamoto;Mamoru Fujitsuka;T. Tachikawa;T. Tachikawa;T. Tachikawa;T. Tachikawa;T. Tachikawa;T. Tachikawa;s. Tojo;S. Tojo;T. Tachikawa;T. Tachikawa;X. Cai;M. Sakamoto;X. Cai;M. Sakamoto;T. Tachikawa;T. Tachikawa;T. Tachikawa;T. Tachikawa;T. Tachikawa;S. Tojo;S. Tojo;T. Tachikawa;X. Cai;M. Sakamoto;M. Sakamoto;Tadao Takada;Tadao Takada;Xichen Cai;Masanori Sakamoto;Kiyohiko Kawai
• 通讯作者: Kiyohiko Kawai