Molecular devices by functionalization of modified DNA

本研究的目的是利用与各种功能发色团共价修饰的DNA赋予修饰DNA的各种功能，建立对这些功能的控制，并阐明修饰DNA作为生物系统分子器件的可能性。特别是，为了阐明DNA中与DNA分子线结构相关的空穴转移，在纳米尺度上根据核碱基序列研究了具有光功能发色团的修饰DNA的光功能。为了阐明纳米尺度和纳米-皮秒时间尺度上的电荷分离、空穴迁移和电荷重组，我们不仅对一个发色团进行修饰，还对多个发色团进行修饰。自下而上的纳米技术需要通过分子组装对功能分子进行精确操作的技术，需要建立基于人工信息的分子组装方法。DNA是最强大的分子，它可以与许多种类的分子进行精确的分子组装，并且具有通过对DNA进行化学修饰可以引入多种功能分子的优势。因此，我们合成了具有分支分裂结构的DNA单元，构建了基于自组装的规则刚性DNA结构，并成功构建了DNA纳米结构，利用这种结构构建了桥梁DNA。为了更精确地通过修饰DNA的功能化来建立分子器件，实现叶绿素、光功能和复合功能等多功能是非常重要的。阐明构建具有高性能和具有生物分子功能的修饰DNA分子器件的方法，开拓修饰DNA分子器件应用于活体系统的可能性。

In this research, it is aimed that various functionalities are given to modification DNA by using DNA modified covalently with various functional chromophores, controlling the functionalities can be established, and the possibility of modified DNA as molecular devices in the living body system is clarified. Especially, in order to clarify the hole transfer in DNA with respect to the construction of DNA molecular wire, photofunctions of modified DNA having photofunctional chromophores has been studied in the nano meter scale depending on the nuclear base sequences. Not only one chromophore but also several chromophores are modified on one DNA in order to clarify the charge separation, hole migration, and charge recombinataion in the nano meter scale and in the nano-pico second time scale. Technology that accurately operates functional molecule by means of molecular assembly is necessary to the nanotechnology of a bottom-up type, and it is necessary to establish the method of molecular assembly according to artificial information. DNA is the most powerful molecule where an accurate molecular assembly is achieved with many kinds of molecules and has the advantage that a variety of functional molecules can be introduced by the chemical modification in DNA. Therefore, DNA unit with the branch dividing structure was synthesized to construct a regular and rigid DNA structures based on self-assembly, and we succeeded to construct DNA nano structure to use this constructing a bridge DNA. In order to establish molecular devices by functionalization of modified DNA more precisely, to achieve the multifunction like the chlorophyll, photofunction, and complex functions is very important. It is also necessary to clarify the method of construction of modified DNA with a high performance and molecular device as a biomolecular function, and to develop the possibility of modified DNA as molecular devices in the living body system.