Smooth muscle regulation

平滑肌调节

• 批准号: 13307005
• 负责人: KOHAMA Kazuhiro
• 金额: $ 32.12万
• 依托单位: Gunma University
• 依托单位国家: 日本
• 项目类别: Grant-in-Aid for Scientific Research (A)
• 财政年份: 2001
• 资助国家: 日本
• 起止时间: 2001 至 2003
• 项目状态: 已结题
• 来源: https://kaken.nii.ac.jp/grant/KAKENHI-PROJECT-13307005/
• 关键词: Smooth muscle; regulation; myosin; myosin light chain kinase; down-regulation

Myosin light chain kinase (MLCK) is a regulatory protein for smooth muscle contraction, which acts by phosphorylating 20-kDa myosin light chain (MLC20) to activate the myosin ATPase activity. Although this mode of action is well-established, there are numerous reports of smooth muscle contraction that is not associated with MLC20 phosphorylation. The kinase activity for the phosphorylation is localized at the central part of MLCK, which is also furnished with actin-binding activity at its N terminal and myosin-binding activity at its C terminal. The present study shows how such multifunctional properties of MLCK modify the actin-myosin interaction and presents our observations that the phosphorylation is not obligatory in induction of smooth muscle contraction.

肌球蛋白轻链激酶 (MLCK) 是平滑肌收缩的调节蛋白，通过磷酸化 20-kDa 肌球蛋白轻链 (MLC20) 来激活肌球蛋白 ATP 酶活性。尽管这种作用模式已被广泛接受，但仍有大量关于平滑肌收缩与 MLC20 磷酸化无关的报道。磷酸化激酶活性位于 MLCK 的中心部分，其 N 端还具有肌动蛋白结合活性，C 端具有肌球蛋白结合活性。本研究表明 MLCK 的这种多功能特性如何改变肌动蛋白-肌球蛋白相互作用，并提出我们的观察结果，即磷酸化在诱导平滑肌收缩中不是必需的。

项目成果

Y.Gao, K.Kawano, S.Yoshiyama, H.Kawamiti, X.Wang, A.Nakamura, K.Kohama: "Myosin light chain kinae stimulates smooth muscle myosin ATPase activity by binding to the myosin heads without phosphorylating the myosin light chain."Biochem.Biophys.Res.Commun.. 3

Y.Gao、K.Kawano、S.Yoshiyama、H.Kawamiti、X.Wang、A.Nakamura、K.Kohama：“肌球蛋白轻链激酶通过与肌球蛋白头结合而不磷酸化肌球蛋白轻链来刺激平滑肌肌球蛋白 ATP 酶活性

W.Iwasaki, H.Sasaki, A.Nakamura, K.Kohama, M.Tanokura: "Metal-Free and Ca^<2+>-Bound Structures of a Multidomain EF-Hand Protein, CBP4O, from the Lower Eukaryote Physarum polycephalum"Structure. 11. 75-85 (2002)

W.Iwasaki、H.Sasaki、A.Nakamura、K.Kohama、M.Tanokura：“来自低等真核生物多头绒泡菌的多域 EF-Hand 蛋白 CBP4O 的无金属和 Ca^2> 结合结构”

K.Gao, L.-H.Ye, H.Kishi, T.Okagaki, K.Samizo, A.Nakamura, K.Kohama: "Myosin light chain kinase as a multifunctional regulatory protein of smooth muscle contraction"IUBMB Life. 51. 337-344 (2001)

K.Gao、L.-H.Ye、H.Kishi、T.Okagaki、K.Samizo、A.Nakamura、K.Kohama：“肌球蛋白轻链激酶作为平滑肌收缩的多功能调节蛋白”IUBMB Life。

S.Kato, E.Aihara, A.Nakamura, H.Xin, H.Matsui, K.Kohama, K.Takeuchi: "Expression of vanilloid receptors in rat gastric epithelial cells : role in cellular protection."Biochem.Pharmacol.. 66. 1115-1121 (2003)

S.Kato、E.Aihara、A.Nakamura、H.Xin、H.Matsui、K.Kohama、K.Takeuchi：“大鼠胃上皮细胞中香草酸受体的表达：在细胞保护中的作用。”Biochem.Pharmacol..

S.Tsutumi, A.Tomioka, M.Sudo, A Nakamura, K.Shirakawa, K.Takegushi, K.Kohama: "Propofol activates vanilloid receptor channels expressed in human embryonic kidney 293 cells."Neurosci.Lett.. 312. 45-49 (2001)

S.Tsutumi、A.Tomioka、M.Sudo、A Nakamura、K.Shirakawa、K.Takegushi、K.Kohama：“异丙酚激活人胚胎肾 293 细胞中表达的香草酸受体通道。”Neurosci.Lett.. 312. 45