Influence of endocrine disrupting chemicals on the health of future generation

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[Cohort study from pregnant women](1) Subjects were pregnant women between gestation weeks 26 and 35, and 340 was recruited until Feb. 2004. (2)Diet, home environment, etc. were included in a questionnaire to pregnant women. (3) Their infants were assessed by neurobehavioral tests : the Bayley Scales of Infant Development at 6 and 18 months old, and the Fagan Test of Infant Intelligence at 7 months old. (4) Levels of FT4 and TSH of pregnant women and newborns were measured. (5) A level of IgE of cord blood was measured. (6) Levels of dioxin and PCBs of 100 pregnant women were measured. Levels of PFOS of fifteen pairs of maternal and cord blood samples were measured. PFOS accumulation could be measured in human and there was a high correlation between PFOS concentrations in maternal and cord blood. (7) Gene polymorphisms of xenobioticTinetabolizing enzymes were investigated. (8) Expression of P450 and exposure of endocrine disrupting chemicals in the placentas were investigated. (9) These infants will be followed up for several years, and influence of endocrine disrupting chemicals on their neurobehavioral development and allergic diseases will be investigated.[Case-control studies of hypospadias and cryptorchidism]Case-control studies of hypospadias and cryptorchidism were performed in the university hospital, its related hospitals in the city of Sapporo, and other hospitals outside Hokkaido. (1) To examine the association between hypospadias and polymorphisms in steroid-metabolizing enzymes, Boys with S allele (G289S) of the HSD17B3 gene had an increased risk of hypospadias, especially distal types. Boys with V allele (L89V) of the SRD5A2 gene had a decreased risk of hypospadias, but not significant. (2) In mothers' heterozygote of Mspl polymorphism in the 3'-flanking region of CYP1A1 gene, odds ratio for hypospadias decreased significantly Similar tendency was observed in mothers' heterozygote of CYP1A1 gene for cryntorchidism, but not significant.