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ARTIFICIAL EXTRACELLULAR MATRIX MADE OF ALGINATE

海藻酸盐制成的人工细胞外基质

基本信息

批准号：
13308054
负责人：
TANIHARA Masao
金额：
$ 14.39万
依托单位：
NARA INSTITUTE OF SCIENCE AND TECHNOLOGY
依托单位国家：
日本
项目类别：
Grant-in-Aid for Scientific Research (A)
财政年份：
2001
资助国家：
日本
起止时间：
2001 至 2003
项目状态：
已结题

项目摘要

In this research, we aimed to develop an artificial extracellular matrix for tissue and organ repair, mimicking the molecular structure and functions of the extracellular matrix in multi cellular organisms.1.Bone repair by the alginate artificial extracellular matrixA novel bone-forming peptide (BFP) designed based on the structure of BMP-2 bound to cell surface receptor, increased ALP activity, and increased expression of osteocalcin of bone precursor cells. ABFP-alginate gel conjugate induced ectopic bone formation when it was implanted into the rat calf muscle, and also showed accelerated bone repair in the rabbit ulnar defects with 25mm length after 9-weeks implantation.2.Slow release of heparin-binding growth factors from heparin/alginate artificial extracellular matrixWe showed sustained release of the heparin-binding growth factors, such as bFGF, HGF, VEGF, and so on, from a novel heparin/alginate artificial extracellular matrix. In animal experiments, the material increased rep … More air of blood vessels, skins, nerves, and kidney.3.Cell-death controlling materialWe create novel cell death-inhibiting peptides based on the structural homology of the death ligand family, TNFα FasL, and TRAIL. The TNFα-inhibiting peptide *owed marked cell survival and differentiation of hippocampal neural stem cells to neuronal cells. The peptide showed also differentiation of bone marrow stromal cells to neuronal cells.4.Apatite-alginate gel 3D-hybridIntroduction of silanol groups into alginate gave 3D apatite-alginate hybrid using biomimetic process. This hybrid material accelerated repair of rat tibial bone defects.ConclusionThe alginate artificial extracellular mix revealed to act as a cell scaffold, to stabilize and release of growth factors, and to control cell growth, differentiation and death. Based on these functions of the alginate artificial extracellular matrix we developed useful materials for the repair of bone, nerve, skin, blood vessels, and kidney. Further investigation will make the alginate artificial extracellular matrix to make useful materials for tissue engineering and regenerative medicine. Less

在本研究中，我们的目标是开发一种用于组织和器官修复的人工细胞外基质，模拟多细胞组织中细胞外基质的分子结构和功能。1.海藻酸盐人工细胞外基质骨修复基于BMP-2与细胞表面受体结合的结构设计的新型骨形成肽(BFP)，增加骨前体细胞的碱性磷酸酶(ALP)活性和骨钙素的表达。2.肝素/海藻酸盐人工细胞外基质缓释肝素结合生长因子从一种新型肝素/海藻酸盐人工细胞外基质中释放肝素结合生长因子在动物实验中，这种材料增加了REP…更多的血管、皮肤、神经和肾脏的空气。3.细胞死亡控制材料基于死亡配体家族、肿瘤坏死因子α、FasL和TRAIL的结构同源性，我们创造了新的细胞死亡抑制多肽。肿瘤坏死因子α抑制肽具有显著的细胞存活和海马神经干细胞向神经细胞分化的作用。海藻酸盐凝胶3D-杂交法将硅醇基团引入到海藻酸盐中，利用仿生方法得到了三维磷灰石-海藻酸盐杂化材料。结论藻酸盐人工细胞外基质可作为细胞支架，稳定和释放生长因子，控制细胞的生长、分化和死亡。基于藻酸盐人工细胞外基质的这些功能，我们开发了用于骨、神经、皮肤、血管和肾脏修复的有用材料。进一步的研究将使藻酸盐人工细胞外基质成为组织工程和再生医学的有用材料。较少