Transcriptional regulation of the carbohydrate metabolism of the facultatively heterotrophic crenarchaeote Thermoproteus tenax

兼性异养crearchaeote Thermoproteus tenax碳水化合物代谢的转录调控

• 批准号: 5314198
• 负责人: Professor Dr. Reinhard Hensel (†)
• 金额: --
• 依托单位: Arbeitsgruppe Molekulare Enzymtechnologie und Biochemie
• 依托单位国家: 德国
• 项目类别: Priority Programmes
• 财政年份: 2001
• 资助国家: 德国
• 起止时间: 2000-12-31 至 2007-12-31
• 项目状态: 已结题
• 来源: https://gepris.dfg.de/gepris/projekt/5314198?language=en
• 关键词: Transcriptional; regulation; carbohydrate; metabolism; facultatively

The proposed project focuses on the transcriptional regulation of genes encoding the enzymes of the central carbohydrate metabolism of the facultative heterotroph crenarchaeote Thermoproteus tenax. The studies should provide insight into the genetic control mechanisms, which govern the selection of the EmbdenMeyerhof-Parnas (EMP) pathway, the Entner-Doudoroff (ED) pathway, the pentose phosphate (PP) pathway and the oxidative tricarboxylic acid cycle (TCA) for catabolism and determine the direction of the carbon flux through the reversible routes of EMP pathway and TCA cycle in dependence of growth conditions. Our principal aim is to identify regulatory transcription factors, which primarily affect the transcription initiation of genes involved in the central carbohydrate metabolism in response to a carbon source, and to characterize their regulatory effects. As model targets of our investigations, we chose the already characterized genes of the reversible EMP pathway, which show differential mRNA expression under autotrophical and heterotrophical growth conditions. For analyzing the regulatory effects on the transcription initiation, the DNA-binding sites of identified factors will be localized and their specific interaction with their target sites will be determined. Their role in the global control of the central carbon metabolism will be investigated by extending the studies to differentially expressed genes of the other pathway, identified by the ongoing T. tenax genome project.

该项目的重点是对编码兼性异养菌crenarchaeote Thermoproteus tenax的中心碳水化合物代谢酶的基因的转录调控。这些研究将提供深入了解的遗传控制机制，这支配的选择的EmbdenMeyerhof-Parnas（EMP）途径，恩特纳-杜多夫（艾德）途径，戊糖磷酸（PP）途径和氧化三羧酸循环（TCA）的催化剂和确定的方向，通过可逆的路径的EMP途径和TCA循环依赖于生长条件。我们的主要目的是确定调节转录因子，主要影响转录起始的基因参与中央碳水化合物代谢，在响应碳源，并表征其调节作用。作为我们研究的模型目标，我们选择了已经表征的可逆EMP途径的基因，其在自养和异养生长条件下显示出差异mRNA表达。为了分析对转录起始的调控作用，将定位所鉴定因子的DNA结合位点，并确定它们与其靶位点的特异性相互作用。它们在中央碳代谢的全局控制中的作用将通过将研究扩展到其他途径的差异表达基因来研究，这些基因由正在进行的T。Tenax基因组计划。