权益分类	功能权益	普通用户	{{item.name}}会员
{{category.name}}	{{benefitItem.name}}

Adrenocortical tissue formed by transplantation of undifferentiated cells

未分化细胞移植形成的肾上腺皮质组织

基本信息

批准号：
13640668
负责人：
MITANI Fumiko
金额：
$ 2.3万
依托单位：
Kieo University
依托单位国家：
日本
项目类别：
Grant-in-Aid for Scientific Research (C)
财政年份：
2001
资助国家：
日本
起止时间：
2001 至 2002
项目状态：
已结题

来源：
https://kaken.nii.ac.jp/grant/KAKENHI-PROJECT-13640668/
关键词：
stem cell cell transplantation adrenal cortex steroid hormone cell differentiation tissue formation

项目摘要

The adrenal cortex consists of morphologically and functionally different three zones, the zona glomerulosa (zG), the zona fasciculata (zF) and the zona reticularis (zR). Recently we discovered another zone (undifferentiated cell zone : zU) between zG and zF. ; cells in the layer are functionally undifferentiated as judged by their inability to express zone-specific steroidogenic enzymes, P450aldo and P45011β, and are thought to be stem cells for adrenocortical cells. In 1999 we successfully established cell-lines exhibiting properties of such adrenocortical stem cells (AcA 101) utilizing mice carrying a temperature-sensitive SV 40T antigen gene. This study was aimed to investigate the mechanisms of differentiation of the undifferentiated adenocortical cells and the formation and maintenance of cortical zones. We obtained the following results in 2 years (2001 and 2002).1. The undifferentiated cell line (AcA 101) expressed P45011β gene at a non-permissive temperature (39℃) of SV40T antigen synergistically with exposure to dibutyryl cAMP and also at the permissive temperature (33℃) with MATRIGEL consisting of laminin and collagen IV.2. A new secretory protein was identified in AcA 101 by subtractive hybridization. The protein contained a secretory signal, EGF-like repeats, and a proteolytically inactive catepsin B-related sequence.3. The mRNA and its encoded protein were detected only in the zU and zG and not in zF., and the protein was designated as adrenocortical zonation factor 1 (AZ-1).4. Interestingly the expression of AZ-1 in adrenocortical cells is inversely correlated with expression of P45011β.5. We have tried to transplant the AcA101 to the kidney of scid mice, but we have not yet succeeded to obtain the regenerated tissues. We are still investigating the conditions for the transplantation of those cells based on the results obtained so fur.

肾上腺皮质由形态和功能上不同的三个区带组成，即球状皮质（zG）、束状皮质（zF）和网状皮质（zR）。最近，我们发现了zG和zF之间的另一个区域（未分化细胞区：zU）。层中的细胞功能未分化，如通过它们不能表达区域特异性类固醇生成酶P450 aldo和P45011β来判断的，并且被认为是肾上腺皮质细胞的干细胞。1999年，我们利用携带温度敏感SV 40 T抗原基因的小鼠成功地建立了表现出这种肾上腺皮质干细胞（AcA 101）特性的细胞系。本研究旨在探讨未分化腺皮质细胞的分化机制以及皮质带的形成和维持。我们在两年内（2001年和2002年）取得了以下结果。未分化细胞系（AcA 101）在SV 40 T抗原的非允许温度（39℃）下与二丁酰cAMP协同表达P45011β基因，在允许温度（33℃）下与由层粘连蛋白和胶原IV. 2组成的MATRIGEL协同表达P45011β基因。利用消减杂交技术，在AcA 101中鉴定出一个新的分泌蛋白.该蛋白含有一个分泌信号、EGF样重复序列和一个无蛋白水解活性的catepsin B相关序列. mRNA及其编码蛋白仅在zU和zG中检测到，而在zF中未检测到。将该蛋白命名为肾上腺皮质区带化因子1（AZ-1）.有趣的是，AZ-1在肾上腺皮质细胞中的表达与P45011β.5的表达呈负相关。我们曾尝试将AcA 101移植到scid小鼠的肾脏中，但尚未成功获得再生组织。我们仍在研究的条件下移植这些细胞的基础上获得的结果，所以毛皮。