Control of drinking behavior in eels neuronal circuit for swallowing reflex

鳗鱼吞咽反射神经回路饮水行为的控制

基本信息

批准号：
13640681
负责人：
ANDO Masaki
金额：
$ 2.37万
依托单位：
HIROSHIMA UNIVERSITY
依托单位国家：
日本
项目类别：
Grant-in-Aid for Scientific Research (C)
财政年份：
2001
资助国家：
日本
起止时间：
2001 至 2002
项目状态：
已结题

项目摘要

Intracranial administration of angiotensin II (ANG II), acetylcholine (Ach), isoproterenol, and substance P enhanced the drinking rate in a dose-dependent manner. Atrial natriuretic peptide, vasotocin (AVT), bradykinin, ghrelin, and phenylephrine inhibited the drinking rate dose-dependently (Kozaka, Fujii & Ando, 2003). These dipsogenic or antidipsogenic effects were also observed intravenous administration of these ligands (Ando et al., 2002). Similar effects by different administrations suggests that these ligands act on the brain. However, in general, most peptides can not pass through the blood-brain barrier (BBB). So some special sites which lack BBB are required to explain the above results. Such special sites are known as the circumventricular organs (CVOs) in mammals. To search for the CVOs in the eel, Evans blue (EB) was injected intraperitoneally and EB-positive nucleus was detected with fluorescent microscope. EB is well-known to bind serum albumin and the EB-albumin complex does not pass the BBB. The magnocellular preoptic nucleus (PM), anterior tuberal nucleus (ANT) and area postrema (AP) were stained with the EB. The PM was immunoreactive to the antibodies raised against ANG II and AVT, and the AP was stained with anti-tyrosine hydroxlase (TH) antibody (Mukuda, Matunaga & Ando, submitted). Swallowing is due to relaxation of the upper esophageal sphincter (UES) muscle. When EB was injected to the UES muscle, the glossopharyngeal vagal motor complex (GVC) in the medulla oblongate was labeled retrogradely and the GVC was immunoreactive to the antibody raised against choline acetyltransferase (Mukuda & Ando, 2003). Indeed, the UES muscle is innervated by cholinergic neurons (Kozaka and Ando, 2003). The neuronal activity of the GVC was inhibited by catecholamines, and the TH-immunoreactive fibers from the AP projected to the GVC (Mukuda & Ando, in preparation). Brain atlas of the eel was also constructed (Mukuda & Ando, 2003)

颅内给药血管紧张素II（ANG II），乙酰胆碱（ACH），异丙肾上腺素和物质P以剂量依赖性方式提高了饮用率。心房亚硝酸化肽，血管毒素（AVT），松曲蛋白，生长素蛋白和苯肾上腺素抑制了饮酒率剂量的依赖性依赖性（Kozaka，Fujii＆Ando，2003）。这些配体的静脉内给药还观察到了这些次源性或抗体生成作用（Ando等，2002）。不同行政部门的类似影响表明这些配体对大脑作用。但是，通常，大多数肽无法通过血脑屏障（BBB）。因此，需要一些缺乏BBB的特殊站点来解释上述结果。这些特殊位置被称为哺乳动物中的室内器官（CVO）。为了搜索鳗鱼中的CVO，对腹膜内注射Evans Blue（EB），并使用荧光显微镜检测到EB阳性核。 EB是结合血清白蛋白的众所周知，EB-Albumin络合物不会通过BBB。用EB染色了巨细胞前核（PM），前结节核（ANT）和区域postrema（AP）。 PM对针对ANG II和AVT产生的抗体进行免疫反应，AP用抗酪氨酸羟化酶（Th）抗体（Mukuda，Matunaga＆Ando，已提交）染色。吞咽是由于食管上括约肌（UES）肌肉的放松。当将EB注入UES肌肉时，将髓质的舌咽迷走运动复合物（GVC）标记为逆行标记，并且GVC对针对胆碱乙酰转移酶的抗体进行了免疫反应（Mukuda＆Ando，2003）。实际上，UES肌肉受到胆碱能神经元的支配（Kozaka and Ando，2003）。 Catecholamine抑制了GVC的神经元活性，以及从预测到GVC的AP（Mukuda＆Ando，准备制备）的TH-免疫反应纤维。还建造了鳗鱼的脑图（Mukuda＆Ando，2003）