Molecular Breeding of Disease Resistant Plants with Regulation of Small G-protein, Rac.
通过调节小 G 蛋白 Rac 进行抗病植物的分子育种。
基本信息
- 批准号:13660048
- 负责人:
- 金额:$ 2.37万
- 依托单位:
- 依托单位国家:日本
- 项目类别:Grant-in-Aid for Scientific Research (C)
- 财政年份:2001
- 资助国家:日本
- 起止时间:2001 至 2002
- 项目状态:已结题
- 来源:
- 关键词:
项目摘要
A small G-protein, Rac, has been known as a key regulator of immune system mediated by the cytoskeleton and NADPH oxidase in animals. In plant defense system, details of Rac functions are still unclear, although a possibility that plant Rac proteins regulate superoxide generation in infected tissues has been pointed out. In this research, to determine the function of plant Rac proteins in defense responses, systemic aquired resistance (SAR) and local aquired resistance (LAR) of tobacco Rac-overexpressor were investigated. When Rac-overexpressor was inoculated with a pathogenic bacterium, Pseudomonas syringae pv tabaci (Psf), higher level of PR gene was expressed and LAR was induced much quicker than wild type plant inoculated with Pst. Moreover, inoculation of a nonpathogen, Pseudomonas syringae pv glycinea, to lower leaves of Rac-overexpressor cause PR-gene expression in upper leaves. Both acidic PR mRNA and salicylic acid prominently accumulated in the upper leaves of inoculated Rac-overexpressor, whereas no accumulation was detected in a wild type, SR-1, tobacco. These results indicate that SAR was induced in Rac overexpressed tobacco by the inoculation with nonpathogenic Pseudomonas, although the nonpathogen doesn't induce SAR in wild type tobacco. These results also suggest that SA-mediated defense reactions might be easily induced in Rac-overexpressor. To confirm the hypothesis, we investigated SA sensitivity of tobacco plants. SA sensitivity of Rac-overexpressor was enhanced to 10 times higher than wild type tobacco. The enhacement of SA sensitivity should be the main reason of the stronger defense reactions in Rac-overexpressor. Taken together, it is suggested that Rac small G-proteins play an important role in the signal perception of the SA-mediated defense system. Moreover, these results suggest the possibility that Rac genes have promise as a target of molecular bleeding of disease resistant crops.
一种小 G 蛋白 Rac 被认为是动物细胞骨架和 NADPH 氧化酶介导的免疫系统的关键调节剂。在植物防御系统中,Rac 功能的细节仍不清楚,尽管已经指出植物 Rac 蛋白调节受感染组织中超氧化物生成的可能性。在本研究中,为了确定植物 Rac 蛋白在防御反应中的功能,对烟草 Rac 过表达蛋白的系统获得性抗性 (SAR) 和局部获得性抗性 (LAR) 进行了研究。当Rac-overexpressor接种病原菌,Pseudomonas syringae pv tabaci(Psf)时,PR基因表达水平更高,LAR的诱导速度比接种Pst的野生型植物快得多。此外,将非病原体丁香假单胞菌甘氨酸假单胞菌接种到Rac过表达子的下叶中会导致上叶中PR基因的表达。酸性PR mRNA和水杨酸在接种的Rac过表达子的上部叶片中显着积累,而在野生型SR-1烟草中未检测到积累。这些结果表明,通过接种非病原性假单胞菌,在 Rac 过表达的烟草中诱导了 SAR,尽管非病原菌不会在野生型烟草中诱导 SAR。这些结果还表明 SA 介导的防御反应可能很容易在 Rac 过表达蛋白中诱导。为了证实这一假设,我们研究了烟草植物的 SA 敏感性。 Rac 过表达子的 SA 敏感性比野生型烟草高 10 倍。 SA敏感性的增强应该是Rac-overexpressor防御反应增强的主要原因。综上所述,Rac 小 G 蛋白在 SA 介导的防御系统的信号感知中发挥着重要作用。此外,这些结果表明 Rac 基因有希望作为抗病作物分子出血的靶标。
项目成果
期刊论文数量(0)
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KOBAYASHI Issei其他文献
KOBAYASHI Issei的其他文献
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{{ truncateString('KOBAYASHI Issei', 18)}}的其他基金
Omics analysis of factors involving penetration resistance to blast fungus in rice
水稻抗稻瘟病菌渗透影响因素的组学分析
- 批准号:
23580062 - 财政年份:2011
- 资助金额:
$ 2.37万 - 项目类别:
Grant-in-Aid for Scientific Research (C)
MOLECULAR BREEDING OF DISEASE RESISTANT PLANTS WITH ARTIFICIAL CONTROL OF ACTIN RELATED GENE EXPRESSION
人工控制肌动蛋白相关基因表达的抗病植物分子育种
- 批准号:
11660045 - 财政年份:1999
- 资助金额:
$ 2.37万 - 项目类别:
Grant-in-Aid for Scientific Research (C)