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Establishment of environment-conserved-plant-pristection-technology through induced systemic resistance in plauts by beneficial micro organisms

通过有益微生物诱导海藻的系统抗性建立环保植物保护技术

基本信息

批准号：
13660044
负责人：
HYAKUAMCHI Mitsuro
金额：
$ 2.05万
依托单位：
Gifu University
依托单位国家：
日本
项目类别：
Grant-in-Aid for Scientific Research (C)
财政年份：
2001
资助国家：
日本
起止时间：
2001 至 2002
项目状态：
已结题

来源：
https://kaken.nii.ac.jp/grant/KAKENHI-PROJECT-13660044/
关键词：
beneficial microorganisms induced resistarce environment conservation

项目摘要

PGPF isolates from zoysiagrass rhizosphere that promote plant growth were tested for their ability to induce, systemic resistance in cucumber plants against Colletotrichum orbiculare, the anthracnose pathogen. Second true leaves of cucumber plants were treated with culture filtrates (CFs) of these fungal isolates and 24 hrs later third true leaves were inoculated with pathogen. Induced plants significantly reduced the total lesion area compared to uninduced control plants. Value of lignin deposition at points of attempted penetration by the pathogen in induced plants were significantly higher than in control plants. Lignin was formed 24 hrs after pathogen treatment. The elicitor activity of CFs was evaluated in cucumber fruit disks using a luminol-dependent chemiluminescence assay. The CFs of the PGPF isolates elicited a conspicuous superoxide generation from cucumber fruit disks when compared with control. Experiments were also done to determine whether these isolates could activate peroxidase and chitinase genes in cucumber plants or not. Expression of peroxidase and chitinase genes were observed 2 and 4 hrs after each CF-treatment, respectively, suggesting occurrence of priming effect in the cucumber plants. When the hypocotyls of each CF-treated cucumber was challenge inoculated with C. orbiculare, the activation of these genes was more rapid and prominent.

对从结缕草根际分离的促进植物生长的PGPF菌株在黄瓜植株上诱导对炭疽病的系统抗性进行了研究。用这些真菌菌株的培养滤液(CFs)处理黄瓜植株的第二片真叶，24小时后用病原菌接种第三片真叶。与未诱导的对照植株相比，诱导植株显著减少了病斑总面积。诱导植株的木质素沉积值在病原菌试图穿透的点显著高于对照植株。病原菌处理24小时后形成木质素。用鲁米诺依赖的化学发光法测定了CFS在黄瓜果盘中的激发子活性。与对照相比，PGPF分离物的CFs在黄瓜果盘上产生了明显的超氧阴离子。实验还确定了这些菌株是否能够激活黄瓜植株中的过氧化物酶和几丁质酶基因。在每次CF处理后2小时和4小时分别观察到过氧化物酶和几丁质酶基因的表达，表明黄瓜植株发生了启动效应。用轮枝菌挑战接种各处理黄瓜的下胚轴时，这些基因的激活更为迅速和显著。