Production of transgenic plants expressing GroEL gene-Analysis of GroEL gene isolated from Bemisia argentifori endosymbiotic bacteria

表达GroEL基因的转基因植物的产生-银粉虱内共生菌GroEL基因的分析

• 批准号: 13660056
• 负责人: IKEGAMI Masato
• 金额: $ 2.3万
• 依托单位: Tohoku University (2002)Tokyo University of Agriculture (2001)
• 依托单位国家: 日本
• 项目类别: Grant-in-Aid for Scientific Research (C)
• 财政年份: 2001
• 资助国家: 日本
• 起止时间: 2001 至 2002
• 项目状态: 已结题
• 来源: https://kaken.nii.ac.jp/grant/KAKENHI-PROJECT-13660056/
• 关键词: geminivirus; whitefly; Bemisia algentifori; Bemisia tabaci; GroEL; transgenic tobacco; 形質転換タバコ; Bemiscia argentifori; Bemicia argentifori

A GroEL protein produced by the whitefly Bemisia tabaci endosymbiotic bacteria interacts in the insect hemolymph with particles of geminiviruses and it ensure the safe circulative transmission of the virus. Diseases described as tomato leaf curl occur in many parts of tropics and subtropics, ranging from Central America to Mediterranean region, Africa and Australia. They are important causes of loss in yield of tomato crops. Therefore, we have tried the production of the transgenic plants which GroEL gene was introduced. We obtained the following results. (1) We constructed the infectious clone of ToLCV. (2) GroEL gene in B. argentifori (Shizuoka) endosymbiotic bacteria was 1688 bases and the nucleotide sequence was 99% identity with that of B. tabaci (Israel) endosymbiotic bacteria. (3) Transgenic plants introduced into GroEL gene were produced. In future we would like to study the response of the transgenic plants agro-inoculated with ToLCV.

由烟粉虱内共生细菌产生的 GroEL 蛋白在昆虫血淋巴中与双生病毒颗粒相互作用，确保病毒的安全循环传播。番茄卷叶病发生在热带和亚热带的许多地区，从中美洲到地中海地区、非洲和澳大利亚。它们是番茄作物减产的重要原因。因此，我们尝试了导入GroEL基因的转基因植物的生产。我们得到了以下结果。 (1)构建了ToLCV的感染性克隆。 (2)银芽孢杆菌(静冈)内共生菌的GroEL基因为1688个碱基，其核苷酸序列与烟粉虱(以色列)内共生菌的核苷酸序列有99％的一致性。 (3)产生导入GroEL基因的转基因植物。未来我们希望研究农业接种 ToLCV 的转基因植物的反应。

项目成果

Kon et al.: "Genome organization of an infectious clone of Tomato leaf curl virus, a new monoportite Begomovirus"J. Phytopathology. 150. 587-591 (2002)

Kon 等人：“番茄卷曲病毒感染性克隆的基因组组织，这是一种新的单孔 Begomovirus”J.

Samretwanich K. et al: "Complete nucleotide sequence and genome organization of soybean crincle leaf geminivirus"J. phytopath.. 149. 1-4 (2001)

Samretwanich K.等人：“大豆环叶双生病毒的完整核苷酸序列和基因组组织”J.

Suzuki et.al.: "The genome organization of pea stem necrosis virus and its assignment to the Genus Carmovirus"Intervirology. 45. 160-163 (2002)

Suzuki 等人：“豌豆茎坏死病毒的基因组结构及其对 Carmovirus 属的分配”Intervirology。

Kon et.al.: "Genome organization of infectious clone of Tomato leaf curl virus, a new monopartite Begomovirus"J.Phytopath. 150. 587-591 (2002)

Kon 等人：“番茄曲叶病毒（一种新的单部分 Begomovirus）感染性克隆的基因组组织”J.Phytopath。

Ikegami et al.: "Complete nucleotide sequence and the genome organization of patchouli mild mosaic virus RNA1"Intervirology. 44. 355-358 (2001)

Ikegami 等人：“广藿香轻度花叶病毒 RNA1 的完整核苷酸序列和基因组组织”Intervirology。