Virus-vector interactions mediating the whitefly transmission of foregut-borne viruses

病毒-载体相互作用介导粉虱传播前肠传播病毒

• 批准号: 1146797
• 负责人: James Ng
• 金额: $ 54万
• 依托单位: University of California-Riverside
• 依托单位国家: 美国
• 项目类别: Continuing Grant
• 财政年份: 2012
• 资助国家: 美国
• 起止时间: 2012-06-15 至 2020-05-31
• 项目状态: 已结题
• 来源: https://www.nsf.gov/awardsearch/showAward?AWD_ID=1146797&HistoricalAwards=false
• 关键词: Virus; vector; interactions; mediating; whitefly

The epidemiological spread of many insect transmitted plant viruses involves molecular interactions between the virus and a population of cells in the insect host (vector). For successful interactions and virus transmission to occur, specific virus encoded proteins are critical determinants. Virus retention sites are also essential factors and their specific locations within the insect vary among different insect-plant virus systems. The overall HYPOTHESIS to be evaluated is that transmission of foregut-borne viruses, such as the whitefly transmitted viruses in the genus Crinivirus, is mediated by specific virus capsid component(s) and specific retention sites within the foregut of the insects. A novel immunofluorescent localization assay as well as biochemical and molecular approaches will be used to address the following SPECIFIC AIMS: 1) determine if a capsid component, the minor coat protein (CPm), of the crinivirus Lettuce infectious yellows virus (LIYV) can block the specific retention and transmission of LIYV, 2) determine the specific vector retention sites of the crinivirus Lettuce chlorosis virus (LCV), 3) determine if LIYV and LCV co-localize to similar sites in their common whitefly vector, 4) identify, through the use of engineered mutants, the molecular determinants of crinivirus retention and transmission, 5) determine the atomic structure of the LIYV CPm to precisely map the amino acid determinants involved in mediating virus retention and transmission. Results emanating from this project will provide insights into mechanisms underlying the whitefly transmission of criniviruses, and contribute to a broader understanding of virus-vector interactions. This will facilitate the design of blocking agents aimed at controlling the spread of viruses in the future. BROADER IMPACTS: The training opportunities made available through this project will enable trainees of different cultural and socio-economic backgrounds to benefit from the cross-disciplinary and technological/scientific expertise provided by the participating laboratories, as well as from NSF-affiliated programs, such as the CAMP Project, established at and supported by UC Riverside.

许多昆虫传播的植物病毒的流行病学传播涉及病毒与昆虫宿主（载体）中的细胞群之间的分子相互作用。为了成功地发生相互作用和病毒传播，特定的病毒编码蛋白是关键的决定因素。病毒保留位点也是重要因素，并且它们在昆虫内的具体位置在不同的昆虫-植物病毒系统中有所不同。要评估的总体假设是，前肠传播的病毒（例如克里尼病毒属中的烟粉虱传播的病毒）的传播是由特定的病毒衣壳成分和昆虫前肠内的特定保留位点介导的。 一种新型免疫荧光定位测定以及生化和分子方法将用于解决以下具体目标：1）确定crinivirus Lettuce传染性黄化病毒（LIYV）的衣壳成分，小外壳蛋白（CPm）是否可以阻断LIYV的特异性保留和传播，2）确定crinivirus Lettuce的特定载体保留位点 褪绿病毒 (LCV)，3) 确定 LIYV 和 LCV 是否共定位于其常见粉虱载体中的相似位点，4) 通过使用工程突变体，识别克里尼病毒保留和传播的分子决定因素，5) 确定 LIYV CPm 的原子结构，以精确绘制参与介导病毒保留和传播的氨基酸决定因素。该项目的结果将深入了解烟粉虱传播病毒的机制，并有助于更广泛地了解病毒-载体相互作用。这将有助于设计旨在控制未来病毒传播的阻断剂。更广泛的影响：通过该项目提供的培训机会将使不同文化和社会经济背景的学员能够受益于参与实验室提供的跨学科和技术/科学专业知识，以及 NSF 附属项目，例如在加州大学河滨分校建立并支持的 CAMP 项目。