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Clarification of role of silicon on the enhancement of manganese tolerance in higher plants.

阐明硅在增强高等植物锰耐受性方面的作用。

基本信息

批准号：
13660068
负责人：
IWASAKI Kozo
金额：
$ 2.3万
依托单位：
Kochi University
依托单位国家：
日本
项目类别：
Grant-in-Aid for Scientific Research (C)
财政年份：
2001
资助国家：
日本
起止时间：
2001 至 2002
项目状态：
已结题

来源：
https://kaken.nii.ac.jp/grant/KAKENHI-PROJECT-13660068/
关键词：
silicon manganese excess apoplast cell wall beneficial element cowpea pumpkin plant nutrition 台木カボチャ

项目摘要

Mechanisms of Si-enhanced Mn tolerance were investigated in leaves of pumpkin and cowpea.When pumpkin plants were supplied with Si under excess Mn, toxicity symptoms were clearly avoided without decreasing the total Mn. Manganese in the apoplastic washing fluids (AWF) of the plants supplied with Si were lower than 10% of those without Si supply. Fractionation of Mn in the cell walls into exchangeable, Mn oxide, and residual forms indicated that at 2 days after excess Mn treatment, the exchangeable Mn of the plants supplied with Si was larger than that of the plants without Si supply. At 4 days after the treatment, larger amounts of Mn oxide were found for the plants supplied with Si. These plants also showed larger amounts of Si and Mn in the cellulose fraction from their cell walls. Thus, Si supply enhances Mn tolerance of the pumpkin plants by decreasing the apoplastic soluble Mn through the incorporation of excess Mn into the cell walls, in addition to the enhanced adsorption of Mn on, the cell walls.Silicon supply also alleviated Mn toxicity of cowpea without decreasing the total leaf Mn. The Mn in the AWF of the plants supplied with Si was about 40% of those without Si supply. The AWF were extracted from the leaves of the plants treated at different timing of Si supply under excess Mn to examine the relationships between the severities of Mn toxicity and the concentrations of Mn and Si in the AWF. The severities of Mn toxicity were not significantly correlated with Mn in the AWF but were significantly correlated with Si. The results indicated that the decrease of Mn in the AWF caused by Si supply was insufficient to explain the enhanced Mn tolerance in cowpea. An important role of apoplastic soluble Si was suggested.

研究了硅提高南瓜和豇豆叶片对锰的耐受性的机理，结果表明，在锰过量的情况下，硅能明显地避免南瓜的毒性症状，而不降低总锰含量。施硅植株的质外体洗涤液（AWF）中锰含量低于不施硅植株的10%。锰在细胞壁中的分馏成交换性，锰氧化物，和残留形式表明，在过量锰处理后2天，交换性锰的植物硅供应大于植物没有硅供应。在处理后第4天，发现供有Si的植物有更大量的Mn氧化物。这些植物还显示出更大量的Si和Mn的纤维素部分从他们的细胞壁。因此，硅的供应，通过减少质外体可溶性锰掺入到细胞壁，除了增强锰的吸附，细胞壁提高南瓜植株的锰耐性。硅供应还减轻了锰毒豇豆，而不降低叶片总锰。供硅植物的AWF中Mn含量约为不供硅植物的40%。在过量锰条件下，从不同供硅时间处理的植物叶片中提取AWF，以研究AWF中Mn和Si浓度与Mn毒性严重程度之间的关系。锰毒性的严重程度与AWF中的锰无显着相关性，但与硅显着相关。结果表明，硅供应引起的AWF中锰的减少不足以解释豇豆耐锰性的增强。质外体可溶性硅可能起重要作用。