Characterization of His-Asp phosphorelay system in S.pombe and E. coli.

1.裂解酵母His-Asp磷酸传递系统的特性研究发现：(1)裂解酵母基因组序列中的3个组氨酸激酶(HK)冗余地参与G2/M细胞周期调控。(2)HKS分别位于HPT蛋白和反应调节因子Spy1和Mcs4的上游。(3)Prr1作为反应调节因子，参与减数分裂和氧化应激反应。在这一过程中，HKS可能作为氧化应激感受器参与其中。His-Asp磷酸传递系统在大肠杆菌中的特性研究I发现：(1)大肠杆菌中唯一的HPT蛋白YojN在RCSC(HK)和参与包膜合成的反应调节因子(RCSB)之间起磷酸介质的作用。(2)基于对大肠杆菌所有两组分调控系统突变的微阵列分析，His-Asp磷酸传递系统之间可能存在功能相互作用(如串扰)。在这一分析中，我发现细胞功能，如rpos调节子和趋化作用，受到多个His-Asp光传递系统的影响。

1. Characterization of His-Asp phosphorelay system in fission yeastI found that :(1) Three histidine kinases (HK) found in that genome sequence of fission yeast are redundantly involved in the G2/M cell cycle control.(2) The HKs work upstream of Spy1 and Mcs4, the HPt protein and response regulator, respectively.(3) The Prrl, a response regulator, is involved in both meiosis and oxidative stress response. In this process, the HKs may be involved as oxidative stress sensors.2. Characterization of His-Asp phosphorelay system in E. coli.I found that :(1) A sole HPt protein in E. coli, YojN, functions as a phosphate mediator between RcsC (HK) and RcsB (response regulator) that is involved in the capsule synthesis.(2) There may be functional interactions (such as cross talk) among His-Asp phosphorelay systems based on the microarray analysis of all two-component regulatory system mutants of E. coli. In this analysis, I found the cellular functions, such as RpoS regulon and chemotaxis, are affected by multiple His-Asp phoshorelay systems.