Characterization of His-Asp phosphorelay system in S.pombe and E. coli.

粟酒裂殖酵母和大肠杆菌中 His-Asp 磷酸中继系统的表征。

• 批准号: 13660087
• 负责人: AIBE Hirofumi
• 金额: $ 2.24万
• 依托单位: Nagoya University
• 依托单位国家: 日本
• 项目类别: Grant-in-Aid for Scientific Research (C)
• 财政年份: 2001
• 资助国家: 日本
• 起止时间: 2001 至 2002
• 项目状态: 已结题
• 来源: https://kaken.nii.ac.jp/grant/KAKENHI-PROJECT-13660087/
• 关键词: Escherichia coli; fission yeast; signal transduction; micro array; His-Asp phosphorelay; stress response; His-Kinase; Response regulator

1. Characterization of His-Asp phosphorelay system in fission yeastI found that :(1) Three histidine kinases (HK) found in that genome sequence of fission yeast are redundantly involved in the G2/M cell cycle control.(2) The HKs work upstream of Spy1 and Mcs4, the HPt protein and response regulator, respectively.(3) The Prrl, a response regulator, is involved in both meiosis and oxidative stress response. In this process, the HKs may be involved as oxidative stress sensors.2. Characterization of His-Asp phosphorelay system in E. coli.I found that :(1) A sole HPt protein in E. coli, YojN, functions as a phosphate mediator between RcsC (HK) and RcsB (response regulator) that is involved in the capsule synthesis.(2) There may be functional interactions (such as cross talk) among His-Asp phosphorelay systems based on the microarray analysis of all two-component regulatory system mutants of E. coli. In this analysis, I found the cellular functions, such as RpoS regulon and chemotaxis, are affected by multiple His-Asp phoshorelay systems.

1. 裂殖酵母中 His-Asp 磷酸中继系统的表征我发现：（1）裂殖酵母基因组序列中发现的三种组氨酸激酶（HK）冗余地参与 G2/M 细胞周期控制。（2）HK 分别在 HPt 蛋白和反应调节因子 Spy1 和 Mcs4 的上游发挥作用。（3）反应调节因子 Prrl 参与减数分裂。 和氧化应激反应。在此过程中，HKs可能作为氧化应激传感器参与其中。 2．大肠杆菌中His-Asp磷酸传递系统的表征。我发现：（1）大肠杆菌中唯一的HPt蛋白YojN，在RcsC（HK）和RcsB（反应调节剂）之间充当磷酸盐介体，参与胶囊的合成。（2）基于所有化合物的微阵列分析，His-Asp磷酸传递系统之间可能存在功能相互作用（例如串扰）。 大肠杆菌的双组分调节系统突变体。在此分析中，我发现 RpoS 调节子和趋化性等细胞功能受到多个 His-Asp phoshorelay 系统的影响。

项目成果

Takeda, S.: "A novel feature of the multistep phosphorelay in Escherichia coli : a revised model of the RcsC--> YojN--> RcsB signalling pathway implicated in capsular synthesis and swarming behaviour"Mol. Microbiol.. 40. 440-450 (2001)

Takeda, S.：“大肠杆菌多步磷酸中继的一个新特征：涉及荚膜合成和集群行为的 RcsC--> YojN--> RcsB 信号通路的修订模型”Mol。

Aoyama, K.: "Genetic analysis of the His-to-Asp phosphorelay implicated in mitotic cell cycle control : Involvement of histidine-kinase genes of Schizosaccharomyces pombe"Biosci. Biotech. Biochem.. 65. 2347-2352 (2001)

Aoyama，K.：“有丝分裂细胞周期控制中涉及的 His-to-Asp 磷酸中继的遗传分析：粟酒裂殖酵母组氨酸激酶基因的参与”Biosci。

Oshima, T.: "Transcriptome analysis of all two-component regulatory system mutants of Escherichia coli K-12"Mol. Microbiol.. 46. 281-291 (2002)

Oshima, T.：“大肠杆菌 K-12 所有双组分调控系统突变体的转录组分析”Mol。

Mori,H: "Post genome sequence analysis in Escherichia coli"Kagaku to Seibutsu. 40. 469-479 (2002)

Mori,H：“大肠杆菌的后基因组序列分析”Kagaku to Seibutsu。

Takeda, S.: "A novel feature of the multistep phosphorelay in Escherichia coli : a revised model of the RcsC --> YojN --> RcsB signalling pathway implicated in capsular synthesis and swarming behaviour"Mol.Microbiol.. 40. 440-450 (2001)

Takeda, S.：“大肠杆菌多步磷酸中继的一个新特征：涉及荚膜合成和集群行为的 RcsC --> YojN --> RcsB 信号通路的修订模型”Mol.Microbiol.. 40. 440-