Genetic and developmental study of exotic androgenetic Corbicula clam invaded into Corbicula japonica fisheries grand in Tone river.

入侵利根河蚬渔业盛会的外来雄性河蚬的遗传与发育研究。

• 批准号: 13660183
• 负责人: KOMARU Akira
• 金额: $ 2.56万
• 依托单位: Mie University
• 依托单位国家: 日本
• 项目类别: Grant-in-Aid for Scientific Research (C)
• 财政年份: 2001
• 资助国家: 日本
• 起止时间: 2001 至 2002
• 项目状态: 已结题
• 来源: https://kaken.nii.ac.jp/grant/KAKENHI-PROJECT-13660183/
• 关键词: Androgenesis; Exotic species; Meiosis; C.japonica; C.papyracea; C.fluminea; 利根川; ミトコンドリアDNA

We examined the distribution and developmental mode of the exotic Corbicula species invaded into Tone river, i.e.important fisheries cite of C.japonica to estimate the negative genetic and ecological effect. Two exotic species, C.fluminea and C.parparatus were distributed in Tone river. C.fluminea was hermaphrodite and brood the larvae in the gill. C.fluminea reproduced by androgenesis. Judging from histological study on the gonad, C.papyracea was dioecious and viviparity. We can observe the normal development of the fertilized eggs from the sperm from cp and oocytes from C.japonica. The larvae were normally developed at least in to D-shaped veliger larvae. This result suggests possible hybridization can be occurring if two species were distributed in the same area. Mt DNA analysis to construct the phylogenic tree revealed that C.p in tone river might derive from Korean C.papyracea. Fresh water species C.fluminea was very closely related to C.leana, but distantly related to brackish C.papyracea and C.japonica The present study revealed that introduced clam, C.papyracea and C.fluminea may cause the genetic and ecological problems on domestic C.japonica population.

通过对日本刺鱼的重要渔业资源——刺鱼的外来种的分布和发育模式进行分析，估计其对遗传和生态的负面影响。Tone河中分布着两种外来种，fluminea .和parparatus .。氟蝇属雌雄同体，幼虫在鳃中产卵。氟蝇属通过雄激素发生繁殖。从性腺的组织学研究来看，纸草属雌雄异株和胎生。我们可以观察到来自cp的精子和来自粳稻的卵母细胞的受精卵的正常发育。幼虫发育正常，至少为d型幼虫。这一结果表明，如果两个物种分布在同一地区，可能会发生杂交。构建系统发育树的DNA分析表明，tone river的C.p可能来源于韩国的纸草。淡水物种c.f fluinea与c.c leana亲缘关系非常近，但与半咸淡水品种c.c papyracea和c.c japonica亲缘关系较远。本研究表明，引入蛤、c.p papyracea和c.f fluinea可能对国内c.c japonica种群造成遗传和生态问题。

项目成果

Qiu, A., A.Shi., A.Komaru.: "Yellow and brown colour morphs in clam Corbicula fluminea in Sichuan province, China were triploids and tetraplolds"J. Shellfish Res.. 20. 323-328 (2001)

Qiu, A., A.Shi., A.Komaru.：“中国四川省河蚬的黄色和棕色形态为三倍体和四倍体”J.

Ishibshi, R., K.Ookubo, M.Aoki, A.Komaru, K.Kawamura.: "Androgenetic reproduction in a freshwater diploid clam Cubicula fluminea (Bivalvia : Corbiculidae)"Zool. Sci.. (in press). (2003)

Ishibshi, R., K.Ookubo, M.Aoki, A.Komaru, K.Kawamura.：“淡水二倍体蛤 Cubicula Fluminea（双壳纲：蚬科）的雄激素繁殖”动物园。

Ishibashi R, Komaru A, Ookubo K, Kiyomoto M: "The second meiosis occurs in cytochalasin D treated eggs of Corbicula leana even though it is not observed in control androgenetic eggs"Dev. Biol.. 224. 37-43 (2002)

Ishibashi R、Komaru A、Ookubo K、Kiyomoto M：“第二次减数分裂发生在经细胞松弛素 D 处理的蚬蚬卵中，尽管在对照雄激素卵中未观察到这种情况”Dev.

Ishibashi R, Komaru A, Ookubo K and Kiyomoto M: "The second meiosis occurs in cytochalasin D treated eggs of Corbicula leana even though it is not observed in control androgenetic eggs because the maternal chromosomes and centrosomes are extruded at the f

Ishibashi R、Komaru A、Ookubo K 和 Kiyomoto M：“第二次减数分裂发生在经细胞松弛素 D 处理的蚬蚬卵中，尽管在对照雄性卵中未观察到第二次减数分裂，因为母体染色体和中心体在 f 处被挤压。

Ishibashi,R., K.Ookubo, M.Aoki, M.Utaki, A.Komaru and K.Kawamura: "Androgenetic reproduction in a freshwater diploid clam Corbicula fluminea (Bivalvia : Corbiculidae)"Zool.Sci.. in press.

Ishibashi,R.、K.Ookubo、M.Aoki、M.Utaki、A.Komaru 和 K.Kawamura：“淡水二倍体蛤蚬的雄性生殖（双壳纲：蚬科）”Zool.Sci.. 正在出版。