FLUID DYNAMICS OF THE EXCRETORY FLOW OF MUCIN CONTENTS IN RAT GASTRIC GLAND PROCESSED BY HIGH PRESSURE FREEZING/FREEZE SUBSTITUTION.

通过高压冷冻/冷冻替代处理的大鼠胃腺中粘蛋白含量排泄流动的流体动力学。

• 批准号: 13670019
• 负责人: SUGANUMA Tatsuo
• 金额: $ 1.92万
• 依托单位: Miyazaki Medical College
• 依托单位国家: 日本
• 项目类别: Grant-in-Aid for Scientific Research (C)
• 财政年份: 2001
• 资助国家: 日本
• 起止时间: 2001 至 2002
• 项目状态: 已结题
• 来源: https://kaken.nii.ac.jp/grant/KAKENHI-PROJECT-13670019/
• 关键词: high pressure freezing; rat fundic gland; mucin; chief cell; mucous neck cell; electron-histochemistry; lectin; レクチン; ラット胃底線

The high-pressure freezing/freeze substitution sequence followed by Lowicryl K4M embedding provided an excellent ultrastructure and retention of antigenicity of rat gastric glands as well as the intraluminal fluid contents. By taking this advantage, we histochemically investigated the excretory flow of the zymogenic and mucin contents in rat gastric glandular lumen. The combination of KMnO4-UA/Pb staining for zymogenic contents and Griffonia simplicifolia agglutinin (GSA)-II labeling for mucous neck cell (MNC) mucin distinguished the exocytosed zymogenic contents from the MNC mucin in the glandular lumen. Interestingly, at the base and neck regions, the zymogenic contents showed a droplet-like appearance, forming a distinct interface with the MNC mucin. At the pit region, the GSA-II labeling demonstrated restricted paths, designated as MNC mucous channels, which flowed into the surface mucous gel layer. It is noted that the zymogenic contents merged into the MNC mucous channels at this region. At the top pit region, the surface mucous gel layer showed laminated arrays of three types of gastric mucins. Taking together, we propose a model of the excretory flow in rat gastric gland.

高压冷冻/冷冻替代序列和Lowicryl K4M包埋提供了良好的超微结构，保留了大鼠胃腺的抗原性以及管腔内液体内容物。利用这一优势，我们用组织化学方法研究了大鼠胃腺腔内发酵素和粘蛋白含量的排泄流动。酶原含量的KMnO4-UA/Pb染色和粘液颈细胞(MNC)粘蛋白的GSA-II标记相结合，区分了腺腔中MNC粘蛋白和胞外的酶原含量。有趣的是，在底部和颈部，发酵物呈液滴状，与MNC粘蛋白形成明显的界面。在凹坑区域，GSA-II标记显示流入表面粘液凝胶层的受限路径，称为MNC粘液通道。值得注意的是，在该区域，酶原内容融合到MNC粘液通道中。在顶部凹陷区，表面粘液凝胶层显示三种胃粘蛋白的层状排列。综上所述，我们提出了一个大鼠胃腺内排泄物流动的模型。

项目成果

Sawaghchi A., Ishihara K., Kawano J., Oimuna T., Hotta K., Suganuma T.: "Fluid dynamics of the excretory flow of zymogenic and mucin contents in rat gastric gland processed by high-pressure freezing/freeze substitution"J. Histochem. Cytochem.. 50. 223-234

Sawaghchi A.、Ishihara K.、Kawano J.、Oimuna T.、Hotta K.、Suganuma T.：“高压冷冻/冷冻替代处理大鼠胃腺中酶原和粘蛋白含量排泄流的流体动力学”

Sawaguchi A., Ishihara K., Kawano J., Oinuma T., Hotta K., Suganuma T.: "Fluid dynamics of the excretory flow of zymogenic and mucin contents in rat gastric gland processed by high-pressure freezing/freeze substitution"J.Histochem.Cytochem.. 50. 223-234 (

Sawaguchi A.、Ishihara K.、Kawano J.、Oinuma T.、Hotta K.、Suganuma T.：“通过高压冷冻/冷冻替代处理的大鼠胃腺中酶原和粘蛋白含量的排泄流的流体动力学”

Uwatoku R, Suematsu M, Ezaki T, Saiki T, Tsuiji M, Irimura T, Kawada N, Suganuma T, Naito M, Ando M, Matsuno K: "Kupffer Cell-Mediated Recruitment of Rat Dendritic Cells to the Liver : Roles of N-Acetylgalactosamine-Specific Sugar Receptors."Gastroenterol

Uwatoku R、Suematsu M、Ezaki T、Saiki T、Tsuiji M、Irimura T、Kawada N、Suganuma T、Naito M、Ando M、Matsuno K：“库普弗细胞介导的大鼠树突状细胞向肝脏的募集：N的作用

菅沼龍夫, 澤口朗: "凍結技法における新展開-高圧凍結技法"細胞. 35. 106-109 (2002)

Tatsuo Suganuma、Akira Sawaguchi：“冷冻技术的新发展 - 高压冷冻技术”Cell。35. 106-109 (2002)

Sawaguchi A, Ide S, Goto Y, Kawano J, Oinuma J, Suganuma T: "A simple contrast enhancement by potassium permanganate oxidation for Lowicryl K4M ultrathin sections prepared by high pressure freezing/freeze substitution."J Microsc. 201. 77-83 (2001)

Sawaguchi A、Ide S、Goto Y、Kawano J、Oinuma J、Suganuma T：“通过高锰酸钾氧化对通过高压冷冻/冷冻替代制备的 Lowicryl K4M 超薄切片进行简单的对比度增强。”J Microsc。