Immunohistochemical and molecular biological study on GSA-II binding mucin from mucous neck cells

颈部粘液细胞 GSA-II 结合粘蛋白的免疫组织化学和分子生物学研究

• 批准号: 08670024
• 负责人: SUGANUMA Tatsuo
• 金额: $ 0.51万
• 依托单位: Miyazaki Medical College
• 依托单位国家: 日本
• 项目类别: Grant-in-Aid for Scientific Research (C)
• 财政年份: 1996
• 资助国家: 日本
• 起止时间: 1996 至 1997
• 项目状态: 已结题
• 来源: https://kaken.nii.ac.jp/grant/KAKENHI-PROJECT-08670024/
• 关键词: mucus glycoprotein; lectin; GSA-II; fundic gland; mucous neck cell; core protein; gene cloning; glycosidases; 胃底腺; 消化管; 免疫組織化学

(1) Gastric mucus is thought to protect the gastric mucosa from mechanical trauma, desiccation, pathogenic microorganism, acid and proteases. Mucous neck cells of the gastric glands have produced and secreted peculiar mucin different from those of surface mucous epithelial cells. We have purified Griffonia simplicifolia agglutinin (GSA)-II binding mucus glycoprotein (GMG) which is specific to mucous neck cells of rat gastric glands. Since a specific polyclonal antibody against the backbone peptides of GMG has been obtained, we have performed the gene cloning for GMG.So far, several candidate clones have been obtained. The cDNA sequencing is now on progress.(2) Rat gastric glands have also been investigated by glycoconjugate histochemistry using various lectins and glycosidase digestions. In addition to GSA-II,poke weed mitogen (PWM) and Maackia amurensis mitogen (MAM) have also showed the specific labeling of mucous neck cells of rat fundic glands.

(1)胃粘液被认为保护胃粘膜免受机械损伤、干燥、病原微生物、酸和蛋白酶的伤害。胃腺颈粘液细胞产生并分泌与表面粘液上皮细胞不同的特殊粘蛋白。我们纯化了大鼠胃腺粘液颈细胞特异性的Griffonia simplicifolia凝集素（GSA）-Ⅱ结合粘液糖蛋白（GMG）。由于获得了抗GMG骨架肽的多克隆抗体，我们对GMG进行了基因克隆，目前已获得多个候选克隆。cDNA测序正在进行中。(2)大鼠胃腺也进行了研究，糖缀合物组织化学使用各种凝集素和糖苷酶diglysine。除GSA-II外，商陆有丝分裂原（PWM）和怀槐有丝分裂原（MAM）也对大鼠胃底腺粘液颈细胞有特异性标记。

项目成果

鳥原康治、森満保、菅沼龍夫、井出惣幸: "モルモット蝸牛におけるポリシアル酸の検討" 耳鼻と臨床. 42. 665-669 (1996)

Koji Torihara、Mitsuyasu Mori、Tatsuo Suganuma、Soyuki Ide：“豚鼠耳蜗中聚唾液酸的检查”Otorhinolaryngology and Clinical Science 42. 665-669 (1996)。

Kawano J, Ide S, Oinuma T, Suganuma T: "Regional distribution of β 1-4 galactosyltransferase in rat epididymis." Acta Histochem.Cytochem.30. 491-496 (1997)

Kawano J、Ide S、Oinuma T、Suganuma T：“大鼠附睾中 β 1-4 半乳糖基转移酶的区域分布。” Acta Histochem.Cytochem.30 (1997)。

菅沼 龍夫: "高圧凍結技法を用いた組織化学" 電子顕微鏡. 32 Suppl.2. 133-136 (1997)

Tatsuo Suganuma：“使用高压冷冻技术的组织化学”电子显微镜 32 Suppl.2 (1997)。

Ponnabalam S: "Primate homologues of rat TGN 38: primary structure, expression and functional implications" J Cell Sci. 109. 675-685 (1996)

Ponnabalam S：“大鼠 TGN 38 的灵长类同源物：一级结构、表达和功能含义”J Cell Sci。

菅沼 龍夫: "組織学一組織化学的アプローチ" 朝倉書店, 310 (1996)

Tatsuo Suganuma：“组织学 - 组织化学方法”朝仓书店，310（1996）