Morphological and functional coupling of IP3 receptors with Na pumps in adrenal chromaffin cells

肾上腺嗜铬细胞中 IP3 受体与 Na 泵的形态和功能耦合

• 批准号: 13670050
• 负责人: INOUE Masumi
• 金额: $ 1.66万
• 依托单位: Fukuoka University
• 依托单位国家: 日本
• 项目类别: Grant-in-Aid for Scientific Research (C)
• 财政年份: 2001
• 资助国家: 日本
• 起止时间: 2001 至 2002
• 项目状态: 已结题
• 来源: https://kaken.nii.ac.jp/grant/KAKENHI-PROJECT-13670050/
• 关键词: Chromaffin cells; Ca store sites; Na pump; α subunit; Ca uptake; IP3 receptor; Catecholamine; secretion; ウァバイン; Ca動員取り込み

Adrenal chromaffin cells are exposed to high concentrations of cortical hormones, one of which is ouabain-like substance. Thus, the effects of ouabain on catecholamine secretion and distribution of Na^+, K^+-ATPase α subunits in chromaffin cells were examined using amperometry and immunocytochemistry. While exposure to 1 μM ouabain did not have a marked effect on resulting secretion, it induced an increase in the secretion due to mobilization of partially stored Ca^<2+>. Immunocytochemistry revealed that Na^+, K^+-ATPase α1 subunit-like immunoreactivity (IR) was distributed ubiquitously at cell periphery, whereas α2-like IR were present at part of cell periphery. Inositol trisphosphate receptor (InsP_3R) type 2-like IR was distributed perinuclearly and in the vicinity of the plasma membrane, consistent with that of BODIPY-FL-InsP_3 binding. Peripheral BODIPY-FL-InsP_3 binding sites underlay membranes with α2-like IR, indicating that the peripheral InsP_3R type 2-containing Ca^<2+> storage was juxtaposed to membranes with the α2 subunit. This close association may account for the facilitation of Ca^<2+> uptake into stores and the consequent increase in mobilization-dependent secretion in the presence of 1 μM ouabain.

肾上腺嗜铬细胞暴露于高浓度的皮质激素，其中之一是哇巴因样物质。因此，我们用电流法和免疫细胞化学方法研究了哇巴因对嗜铬细胞分泌儿茶酚胺和Na^+，K^+-ATP酶α亚基分布的影响。虽然暴露于1 μM哇巴因对产生的分泌没有显著影响，但由于动员了部分储存的Ca^<2+>，它诱导了分泌的增加。免疫细胞化学显示Na^+，K^+-ATP酶α1亚基样免疫反应阳性细胞广泛分布于细胞周边，而α2亚基样免疫反应阳性细胞仅分布于部分细胞周边。InsP_3受体2型分布于细胞核周围和细胞膜附近，与BODIPY-FL-InsP_3的结合相一致。外周BODIPY-FL-InsP_3结合位点覆盖具有α2样IR的膜，表明外周InsP_3R 2型的Ca^2+储存与具有α2亚基的膜并列。这种密切的联系可能是1 μM哇巴因促进Ca^2+摄取到钙库中以及随后增加动员依赖性分泌的原因。

项目成果

井上真澄: "ブレインサイエンス・レビュー2001"医学書院. 98-111 (2001)

井上真澄：《脑科学评论 2001》 Igaku Shoin 98-111 (2001)。

Inoue M, Fujishiro N, Imanaga I, Sakamoto Y: "Role of ATP decrease in secretion induced by mitochondrial dysfunction in guinea-pig adrenal chromaffin cells"J Physiol.. 539. 145-155 (2002)

Inoue M、Fujishiro N、Imanaga I、Sakamoto Y：“豚鼠肾上腺嗜铬细胞线粒体功能障碍诱导的 ATP 分泌减少的作用”J Physiol.. 539. 145-155 (2002)

M.Inoue et al.: "Role of ATP decrease in secretion induced by mitochondrial dysfunctionin guinea-pig adrenal chromaffin cells"Journal of Physiology. 539. 145-155 (2002)

M.Inoue 等人：“豚鼠肾上腺嗜铬细胞线粒体功能障碍诱导的 ATP 分泌减少的作用”生理学杂志。

M. Inoue, Y. Sakamoto, N. Fujishiro, I. Imanaga, S. Ozaki, G. D. Prestwich, A. Warashima: "Homogenous Ca store sites in rat adrenal chromaffin cells"Cell Calcium. 33. 19-26 (2003)

M. Inoue、Y. Sakamoto、N. Fujishiro、I. Imanaga、S. Ozaki、G. D. Prestwich、A. Warashima：“大鼠肾上腺嗜铬细胞中的均质 Ca 存储位点”细胞钙。

M. Inoue, N. Fujishiro, I. Imanaga, Y. Sakamoto: "Role of ATP decrease in secretion induced by mitochondrial dysfunction in guinea-pig adrenal chromaffin cells"J. Physiol. Lond.. 539. 145-155 (2002)

M. Inoue、N. Fujishiro、I. Imanaga、Y. Sakamoto：“豚鼠肾上腺嗜铬细胞线粒体功能障碍诱导的 ATP 分泌减少的作用”J。