口腔乾燥症モデル動物の確立と義歯床下粘膜のダメージおよび治癒過程に関する検討

口干症模型动物的建立及义齿基托下黏膜损伤及愈合过程的研究

• 批准号: 13771161
• 负责人: 岡本 信
• 金额: $ 1.79万
• 依托单位: Okayama University
• 依托单位国家: 日本
• 项目类别: Grant-in-Aid for Young Scientists (B)
• 财政年份: 2001
• 资助国家: 日本
• 起止时间: 2001 至 2002
• 项目状态: 已结题
• 来源: https://kaken.nii.ac.jp/grant/KAKENHI-PROJECT-13771161/
• 关键词: 口腔乾燥症; 唾液分泌量; ラット; 病理組織; 咀嚼圧; 唾液腺; AgNOR染色; 粘膜上皮活性

1.口腔乾燥症モデル動物の確立10週齢のウィスター系雄性ラット40匹(10匹×4群)を用いた.腹腔内麻酔下において唾液腺の摘出を行った.1群には,耳下腺導管の結紮を行い.また,1群には,耳下腺導管結紮および,顎下腺,舌下腺の摘出を行う.その他の1群には偽手術を行い,残りの1群は無処置のまま経過させた.手術5週後から,1週間ごとに唾液量(ろ紙を用いて測定),水分摂取量,食物摂取量,行動の変化を観察した.手術10週後にすべての動物を屠殺し,口蓋組織,歯周組織舌を採取した.採取した組織はヘマトキシリン-エオジン染色を施した.組織観察の結果と唾液分泌量他の所見を考慮すると,モデル動物としては,耳下腺導管結紮,顎下腺・舌下腺摘出を行った動物が最も適しており,術式としても問題なく実験に供することができると判断された.2.実験的口腔乾燥症下における義歯床下粘膜のダメージと治癒過程の観察10週齢のウィスター系雄性ラット125匹(25匹×5群)を用いた.耳下腺結紮・顎下腺・舌下腺摘出により口腔乾燥症ラットを75匹作製した.それらのうち2群の動物には,咀嚼圧を加える実験用義歯を装着した(13μm,50μm沈下群).残りの1群は実験用義歯を装着せずに経過させた.また,1群25匹の動物には偽手術を施し,残りの1群25匹は無処置のまま経過させた.手術5週後に実験用義歯を装着し,義歯装着1週後から1週間隔で5週後までを観察期間とした.各観察期間の終了時に,各実験群の各5匹を環流固定して,義歯床下の口蓋組織を採取した.採取した組織は固定,脱灰ののち,パラフィン包埋し,ヘマトキシリン-エオジン染色およびAgNOR染色を施した.組織観察ならびに評価・検討は来年度の予定となっている.

1. The animals of xerostomia were treated with 40 male animals (10 dogs x 4 groups). The salivary glands were removed from the abdominal cavity under anesthesia. 1 group, the subauricular gland tube was removed. The subauricular gland, sublingual gland and sublingual gland were removed. One group of handicapped workers and one group of handicapped workers have passed the training program. After 5 years of surgery, the amount of saliva was measured in 1 month, the amount of water, the amount of food, the amount of saliva, the amount of water, the amount of food, the amount of saliva, the amount of water, the amount of food, the amount of saliva, the amount of water, the amount of food, the amount of saliva, the amount of water and food were measured within 1 month. After 10 years of surgery, the animals were slaughtered, the oral tissue and the tongue were collected. This is the first time that the tissue has been stained and applied. The results of tissue examination showed that the amount of saliva secreted by others was measured, the amount of saliva secreted by others, the amount of food in the animal, the tube of the subauricular gland, the sublingual gland of the sublingual gland were removed, and the animal was extracted from the sublingual gland. 2. During the treatment of xerostomia, a total of 125 males (25 horses x 5 groups) were used. The subauricular gland was extracted from the sublingual gland of the subauricular gland. Make sure that there are two groups of animals, chew them, and use them to hold them (13 μ m, 50 μ m). The handicapped group of people used to install the equipment to pass through the train. One group of 25 animals were operated, and the disabled group of 25 animals were tested. After 5 years of surgery, the equipment is installed in a tube, and the equipment is installed after 1 week, and after 5 years, it is inspected during the period. Time has been spent during each inspection period, and each cluster has 5 fixed ambient flow stations, and the mouth under the bed has been organized to collect data. The tissue was fixed, the ash was removed, the tissue was embedded, and the tissue was stained by AgNOR staining. The organization is expected to make plans for the coming year.