Analysis of Morphogenesis by using molecular methods on Histrid beetles

用分子方法分析 Histrid 甲虫的形态发生

• 批准号: 13839003
• 负责人: OHARA Masahiro
• 金额: $ 1.22万
• 依托单位: Hokkaido University
• 依托单位国家: 日本
• 项目类别: Grant-in-Aid for Scientific Research (C)
• 财政年份: 2001
• 资助国家: 日本
• 起止时间: 2001 至 2002
• 项目状态: 已结题
• 来源: https://kaken.nii.ac.jp/grant/KAKENHI-PROJECT-13839003/
• 关键词: Histeridae; Molecular phylogeny; Coleoptera; 28S; 16S; ND2

As materials of this project, 60 species of the family Histeridae (Coleoptera) were available for phylogenetic molecular analysis, the materials were collected in Japan and Indonesia, and also contributed by many scientists of the world. Of them, 5 families, 8 subfamilies, 29 genera and 49 species have been analyzed their DNA. Molecular analysis of this project were used nuclear 28S, mitochondria ND2 and 16S. It was achieved rather good results of 28S, then I constructed the phylogeny of histrid beetles by using 28S, mainly.Phylogeny of the family Histeridae is constructed. The tree shows monophyly of some tribes, but on some taxa, e.g. Dendrohilinae, non-monophyly. Bootstrap value is low of the tree among subfamilies. Topology of the molecular tree, however, shows consistency with morphological phylogeny.It is difficult to show the mechanisms of morphogenesis of the family Histeridae. Because analysis of the mechanisms is depended on the result of phylogenetic analysis. I found some consistency and inconsistency between molecular and morphological trees under this project. Such inconsistencies will be clue to resolve parallelism and convergence on the beetles. Dr. Mazur and I gathered information of biology of the histrid beetles. The information will be useful as mapping characters on trees in further study.

作为本项目的材料，可用于系统发育分子分析的Histeridae科（鞘翅目）60种材料，材料收集于日本和印度尼西亚，也得到了世界许多科学家的贡献。其中，5科、8亚科、29属、49种的DNA已被分析。本项目的分子分析分别使用了核28S、线粒体ND2和16S。 28S取得了较好的结果，接下来我主要利用28S构建了组甲虫的系统发育树。构建了组甲虫科的系统发育树。该树显示了一些部落的单系性，但在一些分类单元上，例如树突亚科，非单系。子科中树的 Bootstrap 值较低。然而，分子树的拓扑结构与形态系统发育显示出一致性。很难揭示Histeridae科的形态发生机制。因为机制的分析取决于系统发育分析的结果。我发现这个项目下的分子树和形态树之间存在一些一致性和不一致之处。这种不一致将为解决甲虫的并行性和收敛性提供线索。马祖尔博士和我收集了三甲虫的生物学信息。这些信息将有助于在进一步的研究中绘制树木上的特征。