Analysis of Morphogenesis by using molecular methods on Histrid beetles

用分子方法分析 Histrid 甲虫的形态发生

• 批准号: 13839003
• 负责人: OHARA Masahiro
• 金额: $ 1.22万
• 依托单位: Hokkaido University
• 依托单位国家: 日本
• 项目类别: Grant-in-Aid for Scientific Research (C)
• 财政年份: 2001
• 资助国家: 日本
• 起止时间: 2001 至 2002
• 项目状态: 已结题
• 来源: https://kaken.nii.ac.jp/grant/KAKENHI-PROJECT-13839003/
• 关键词: Histeridae; Molecular phylogeny; Coleoptera; 28S; 16S; ND2

As materials of this project, 60 species of the family Histeridae (Coleoptera) were available for phylogenetic molecular analysis, the materials were collected in Japan and Indonesia, and also contributed by many scientists of the world. Of them, 5 families, 8 subfamilies, 29 genera and 49 species have been analyzed their DNA. Molecular analysis of this project were used nuclear 28S, mitochondria ND2 and 16S. It was achieved rather good results of 28S, then I constructed the phylogeny of histrid beetles by using 28S, mainly.Phylogeny of the family Histeridae is constructed. The tree shows monophyly of some tribes, but on some taxa, e.g. Dendrohilinae, non-monophyly. Bootstrap value is low of the tree among subfamilies. Topology of the molecular tree, however, shows consistency with morphological phylogeny.It is difficult to show the mechanisms of morphogenesis of the family Histeridae. Because analysis of the mechanisms is depended on the result of phylogenetic analysis. I found some consistency and inconsistency between molecular and morphological trees under this project. Such inconsistencies will be clue to resolve parallelism and convergence on the beetles. Dr. Mazur and I gathered information of biology of the histrid beetles. The information will be useful as mapping characters on trees in further study.

作为该项目的材料，有60种Histeridae（鞘翅目）的种类可用于系统发育分子分析，这些材料是在日本和印度尼西亚收集的，也由世界许多科学家贡献。其中，已经分析了5个家庭，8个亚科，29属和49种。该项目的分子分析使用核28S，线粒体ND2和16S。这是28s的相当不错的结果，然后我使用28S构建了Histrid甲虫的系统发育，主要是构建Histeridae家族的基因发育。这棵树显示了一些部落的单身性，但在某些分类单元中，例如dendrohilinae，非发行。自举值是亚家族中树的低点。然而，分子树的拓扑表现出与形态系统发育的一致性。很难表现出家族Histeridae的形态发生的机制。因为对机制的分析取决于系统发育分析的结果。我发现该项目下的分子和形态树之间的一致性和不一致。这种不一致将是解决甲虫上并行性和收敛性的线索。 Mazur博士和我收集了Histrid甲虫生物学的信息。在进一步的研究中，该信息将作为在树上绘制字符的映射。