Comprehensive study on regulatory systems for the initiation of secondary metabolism and morphological development in Streptomyces

链霉菌次生代谢启动和形态发育调控系统的综合研究

基本信息

  • 批准号:
    13854005
  • 负责人:
  • 金额:
    $ 77.63万
  • 依托单位:
  • 依托单位国家:
    日本
  • 项目类别:
    Grant-in-Aid for Scientific Research (S)
  • 财政年份:
    2001
  • 资助国家:
    日本
  • 起止时间:
    2001 至 2005
  • 项目状态:
    已结题

项目摘要

1.The overall picture of the A-factor regulatory cascade has been revealed by genetic and biological approaches. For example, the signal transduction from A-factor to the streptomycin biosynthesis genes starts with AfsA that catalyzes the biosynthesis of A-factor from a glycerol derivative and a β-keto acid. Once the intracellular concentration of A-factor reaches a critical level, its information is transferred to ArpA (A-factor receptor and transcriptional repressor) to AdpA (transcriptional activator) to StrR (pathway-specific transcriptional activator for streptomycin biosynthesis) to the streptomycin biosynthesis genes. In addition, X-ray crystallography of ArpA revealed how A-factor dissociates ArpA from the DNA. The detailed study of the hormonal regulation of secondary metabolism and morphological development in Streptomyces, a boundary microorganisms, has given a big impact on microbiology. This study is also useful for further detailed and comprehensive analysis of the A-factor regulatory cascade by using DNA microarray techniques.2.A transcriptional activator AfsR is phosphorylated on its threonine residue(s) by a serine/threonine kinase AfsK. AfsK self-activates its kinase activity by autophosphorylation on Thr-168. The phosphorylated AfsR activates the promoter of afsS, the gene product of which enhances secondary metabolism in some unknown way. The regulatory system involving AfsK/AfsR/AfsS is the most extensively studied system in the bacterial world.3.Catabolite repression by glucose is another regulatory system for secondary metabolism and morphogenesis in Streptomyces. Several mutants that produce antibiotics and spores in a high concentration of glucose were isolated, which I believe will be good material to study catabolite repression. In addition, cAMP and calmodulin-like protein were also found to control morphogenesis of Streptomyces. These regulatory systems will be studied by means of DNA microarray.
1.遗传和生物学方法揭示了A因子调控级联反应的整体图景。例如,从A因子到链霉素生物合成基因的信号转导始于AFSA,它催化从甘油衍生物和β-酮酸生物合成A因子。一旦细胞内A因子浓度达到临界水平,其信息就被传递到ARPA(A因子受体和转录抑制因子)、AdpA(转录激活因子)和STRR(链霉素生物合成途径特异性转录激活因子),传递到链霉素生物合成基因。此外,ARPA的X射线结晶学揭示了A因子是如何将ARPA与DNA解离的。链霉菌作为一种边界微生物,对其次生代谢和形态发育的激素调控进行了详细的研究,对微生物学产生了重大影响。本研究也有助于利用基因芯片技术进一步详细和全面地分析A因子调控级联反应。2.转录激活因子AfsR在其苏氨酸残基(S)上被丝氨酸/苏氨酸激酶AfsK磷酸化。AfsK通过苏氨酸转运蛋白-168上的自动磷酸化来自我激活其激酶活性。AFsR的磷酸化激活AFSS的启动子,AFSS的基因产物以某种未知的方式增强次生代谢。涉及AfsK/AfsR/AFSS的调控系统是细菌界研究最广泛的系统。3.葡萄糖抑制分解代谢是链霉菌次生代谢和形态发生的另一个调控系统。分离到了几个在高浓度葡萄糖中产生抗生素和孢子的突变株,我相信这将是研究分解代谢抑制的很好材料。此外,cAMP和类钙调素蛋白也控制着链霉菌的形态发生。这些调控系统将通过DNA微阵列进行研究。

项目成果

期刊论文数量(143)
专著数量(0)
科研奖励数量(0)
会议论文数量(0)
专利数量(0)
Structure and function of the A-factor receptor (in Japanese).
A 因子受体的结构和功能(日语)。
  • DOI:
  • 发表时间:
    2004
  • 期刊:
  • 影响因子:
    0
  • 作者:
    Sueharu Horinouchi
  • 通讯作者:
    Sueharu Horinouchi
Crystal structure of a γ-butyrolactone autoregulator receptor protein in Streptomyces griseus.
灰色链霉菌中γ-丁内酯自动调节受体蛋白的晶体结构。
  • DOI:
  • 发表时间:
    2004
  • 期刊:
  • 影响因子:
    0
  • 作者:
    N.Sawatari;T.Yokota;S.Sakaguchi;Y.Ishii;Ryo Natsume
  • 通讯作者:
    Ryo Natsume
Biotransformation of flavone and flavanone by Streptomuces lividans cells carrying shuffled biphenyl dioxygenase genes.
携带改组联苯二加氧酶基因的变铅青链霉菌细胞对黄酮和黄烷酮的生物转化。
  • DOI:
  • 发表时间:
    2003
  • 期刊:
  • 影响因子:
    0
  • 作者:
    Hidetada Hirakawa;Yoshihiko Inazumi;Takeshi Masaki;Takahiro Hirata;Akihito Yamaguchi;Hyo-Kon Chun
  • 通讯作者:
    Hyo-Kon Chun
Morphogenesis of Streptomyces, a boundary microorganism (in Japanese).
边界微生物链霉菌的形态发生(日语)。
  • DOI:
  • 发表时间:
    2002
  • 期刊:
  • 影响因子:
    0
  • 作者:
    Y.Kita;M.Matsugi;Sueharu Horinouchi
  • 通讯作者:
    Sueharu Horinouchi
A s^B-like factor responsible for carotenoid biosynthesis in Streptomyces griseus.
灰色链霉菌中负责类胡萝卜素生物合成的 s^B 样因子。
  • DOI:
  • 发表时间:
    2001
  • 期刊:
  • 影响因子:
    0
  • 作者:
    HAMAMOTO;Hiromi;H.Ichibashi;Takaaki Akasaka;Han-Seung Lee
  • 通讯作者:
    Han-Seung Lee
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HORINOUCHI Sueharu其他文献

HORINOUCHI Sueharu的其他文献

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{{ truncateString('HORINOUCHI Sueharu', 18)}}的其他基金

Studies on the structure and function of A-factor receptor based on its X-ray crystallographic structure
基于X射线晶体结构研究A因子受体的结构和功能
  • 批准号:
    11306008
  • 财政年份:
    1999
  • 资助金额:
    $ 77.63万
  • 项目类别:
    Grant-in-Aid for Scientific Research (A).
Japan-UK Joint Study on Molecular Genetics of Streptomyces
日英链霉菌分子遗传学联合研究
  • 批准号:
    09044205
  • 财政年份:
    1997
  • 资助金额:
    $ 77.63万
  • 项目类别:
    Grant-in-Aid for international Scientific Research
Studies on A-factor receptor protein involved in secondary metabolism and morphorogical differentiation in Streptomyces griseus
灰色链霉菌次生代谢及形态分化中A因子受体蛋白的研究
  • 批准号:
    09306005
  • 财政年份:
    1997
  • 资助金额:
    $ 77.63万
  • 项目类别:
    Grant-in-Aid for Scientific Research (A)
Study on the genetics of acetic acid bacteria for the efficient vinegar production
高效制醋醋酸菌的遗传学研究
  • 批准号:
    09556016
  • 财政年份:
    1997
  • 资助金额:
    $ 77.63万
  • 项目类别:
    Grant-in-Aid for Scientific Research (B)
Regulation of secondary metabolism and cell differentiation by A-factor and protein phosphorylation in Streptomyces.
链霉菌中 A 因子和蛋白质磷酸化对次生代谢和细胞分化的调节。
  • 批准号:
    06454073
  • 财政年份:
    1994
  • 资助金额:
    $ 77.63万
  • 项目类别:
    Grant-in-Aid for General Scientific Research (B)
Improvement of milk-clotting enzyme, Mucor rennin, by protein engineering and 「genetic engineering.
通过蛋白质工程和基因工程改进凝乳酶毛霉凝乳酶。
  • 批准号:
    06556014
  • 财政年份:
    1994
  • 资助金额:
    $ 77.63万
  • 项目类别:
    Grant-in-Aid for Developmental Scientific Research (B)
Regulation of secondary metabolism and cellular differentiation by A-factor and signal transduction systems in Streptomyces
链霉菌 A 因子和信号转导系统对次生代谢和细胞分化的调节
  • 批准号:
    04454075
  • 财政年份:
    1992
  • 资助金额:
    $ 77.63万
  • 项目类别:
    Grant-in-Aid for General Scientific Research (B)

相似海外基金

Studies on A-factor receptor protein involved in secondary metabolism and morphorogical differentiation in Streptomyces griseus
灰色链霉菌次生代谢及形态分化中A因子受体蛋白的研究
  • 批准号:
    09306005
  • 财政年份:
    1997
  • 资助金额:
    $ 77.63万
  • 项目类别:
    Grant-in-Aid for Scientific Research (A)
Studies on the structure and rearrangements of the linear chromosome of Streptomyces griseus 2247.
灰色链霉菌2247线性染色体结构和重排的研究。
  • 批准号:
    09460050
  • 财政年份:
    1997
  • 资助金额:
    $ 77.63万
  • 项目类别:
    Grant-in-Aid for Scientific Research (B)
Genetic analysis of pleiotropic mutation independent of A factor derived from Streptomyces griseus
不依赖于灰色链霉菌 A 因子的多效性突变的遗传分析
  • 批准号:
    03454032
  • 财政年份:
    1991
  • 资助金额:
    $ 77.63万
  • 项目类别:
    Grant-in-Aid for General Scientific Research (B)
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