Studies on A-factor receptor protein involved in secondary metabolism and morphorogical differentiation in Streptomyces griseus

灰色链霉菌次生代谢及形态分化中A因子受体蛋白的研究

基本信息

  • 批准号:
    09306005
  • 负责人:
  • 金额:
    $ 18.75万
  • 依托单位:
  • 依托单位国家:
    日本
  • 项目类别:
    Grant-in-Aid for Scientific Research (A)
  • 财政年份:
    1997
  • 资助国家:
    日本
  • 起止时间:
    1997 至 1998
  • 项目状态:
    已结题

项目摘要

A-factor is a representative of the autoregulatory factors, often called microbial hormones, that switch on secondary metabolism and morphogenesis in the bacterial genus Streptomyces. In Streptomyces griseus, A-factor at an extremely low concentration triggers streptomycin (Sm) production and cell differentiation by binding a repressor-type receptor protein (ArpA) and dissociating it from DNA.The purpose of this study is revealing the molecular function of ArpA.1. The target genes of ArpAAn A-factor-responsive transcriptional activator (AdpA) able to bind the promoter of strR, a pathway-specific regulatory gene responsible for transcription of other Sm biosynthetic genes, was found to be the target of ArpA.This result can explain how A-factor triggers Sm biosynthesis at a late exponential growth stage.Two DNA fragments which contain ArpA-binding site were cloned from the genome of S.griseus. Transcriptional analysis of some candidates of ArpA-target gene are proceeding. Gene disruption of these genes are also proceeding.2.3D-structure of a microbial hormone receptorCrystallization of an ArpA homologue (CrpB) of Streptomyces coelicolor was successfully achieved. Crystallographic parameters and initial phases were determined using X-ray diffraction. In near future, 3D-structure of CrpB will be revealed. Then 3D-structure of ArpA may be solved easily.
a因子是自调节因子的代表,通常被称为微生物激素,在细菌属链霉菌中开启次级代谢和形态发生。在灰色链霉菌中,极低浓度的a因子通过结合抑制型受体蛋白(ArpA)并将其与DNA分离,从而引发链霉素(Sm)的产生和细胞分化。本研究的目的是揭示arpa - 1的分子功能。arpaa因子响应性转录激活因子(a -factor-responsive transcriptional activator, AdpA)的靶基因能够结合strR的启动子,strR是负责其他Sm生物合成基因转录的通路特异性调控基因,被发现是ArpA的靶基因。这一结果可以解释a因子如何在指数生长后期触发Sm的生物合成。从稻瘟病菌基因组中克隆出两个含有arpa结合位点的DNA片段。一些候选arpa靶基因的转录分析正在进行中。这些基因的基因破坏也在进行中。2.3微生物激素受体的三维结构成功地实现了链霉菌ArpA同源物(CrpB)的结晶。用x射线衍射测定了晶体学参数和初始相。在不久的将来,CrpB的3d结构将被揭示。这样可以方便地求解ArpA的三维结构。

项目成果

期刊论文数量(39)
专著数量(0)
科研奖励数量(0)
会议论文数量(0)
专利数量(0)
Onaka,H.: "DNA-binding activity of the A-factor receptor protein and its recognition DNA sequences" Mol.Microbiol.24. 991-1000 (1997)
Onaka,H.:“A 因子受体蛋白的 DNA 结合活性及其识别 DNA 序列”Mol.Microbiol.24。
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    0
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Sugiyama,M.: "Site-directed mutagenesis of the A-factor receptor protein : Val-41 important for DNA-binding and Trp-119 important for ligand-binding" Gene. 222. 133-144 (1998)
Sugiyama,M.:“A 因子受体蛋白的定点诱变:Val-41 对 DNA 结合很重要,Trp-119 对配体结合很重要”基因。
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    0
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Noriko Ando: "Gene organization in a 12-Kilobase region covering afsA encoding an A-factor biosynthetic enzyme" Actinomycetol.12. 40-45 (1998)
Noriko Ando:“12-Kilobase 区域的基因组织,覆盖编码 A 因子生物合成酶的 afsA”Actinomycetol.12。
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  • 影响因子:
    0
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  • 通讯作者:
Sueharu Horinouchi: "Cell-Cell Communication in Bacteria" G.M.Dunny and S.C.Winans (eds.) American Society for Microciology,Washington,D.C. (in press), (1998)
Sueharu Horinouchi:“细菌中的细胞间通讯”G.M.Dunny 和 S.C.Winans(编辑)美国微生物学会,华盛顿特区
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    0
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Ueda,K: "Characterization of an A-factor-responsive repressor for amfR essential for onset of aerial mycelium formation in Streptomyces griseus" J.Bacteriol.180. 5085-5093 (1998)
Ueda,K:“amfR 的 A 因子响应阻遏物的表征,对于灰色链霉菌中气生菌丝体形成的开始至关重要”J.Bacteriol.180。
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    0
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HORINOUCHI Sueharu其他文献

HORINOUCHI Sueharu的其他文献

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{{ truncateString('HORINOUCHI Sueharu', 18)}}的其他基金

Comprehensive study on regulatory systems for the initiation of secondary metabolism and morphological development in Streptomyces
链霉菌次生代谢启动和形态发育调控系统的综合研究
  • 批准号:
    13854005
  • 财政年份:
    2001
  • 资助金额:
    $ 18.75万
  • 项目类别:
    Grant-in-Aid for Scientific Research (S)
Studies on the structure and function of A-factor receptor based on its X-ray crystallographic structure
基于X射线晶体结构研究A因子受体的结构和功能
  • 批准号:
    11306008
  • 财政年份:
    1999
  • 资助金额:
    $ 18.75万
  • 项目类别:
    Grant-in-Aid for Scientific Research (A).
Japan-UK Joint Study on Molecular Genetics of Streptomyces
日英链霉菌分子遗传学联合研究
  • 批准号:
    09044205
  • 财政年份:
    1997
  • 资助金额:
    $ 18.75万
  • 项目类别:
    Grant-in-Aid for international Scientific Research
Study on the genetics of acetic acid bacteria for the efficient vinegar production
高效制醋醋酸菌的遗传学研究
  • 批准号:
    09556016
  • 财政年份:
    1997
  • 资助金额:
    $ 18.75万
  • 项目类别:
    Grant-in-Aid for Scientific Research (B)
Regulation of secondary metabolism and cell differentiation by A-factor and protein phosphorylation in Streptomyces.
链霉菌中 A 因子和蛋白质磷酸化对次生代谢和细胞分化的调节。
  • 批准号:
    06454073
  • 财政年份:
    1994
  • 资助金额:
    $ 18.75万
  • 项目类别:
    Grant-in-Aid for General Scientific Research (B)
Improvement of milk-clotting enzyme, Mucor rennin, by protein engineering and 「genetic engineering.
通过蛋白质工程和基因工程改进凝乳酶毛霉凝乳酶。
  • 批准号:
    06556014
  • 财政年份:
    1994
  • 资助金额:
    $ 18.75万
  • 项目类别:
    Grant-in-Aid for Developmental Scientific Research (B)
Regulation of secondary metabolism and cellular differentiation by A-factor and signal transduction systems in Streptomyces
链霉菌 A 因子和信号转导系统对次生代谢和细胞分化的调节
  • 批准号:
    04454075
  • 财政年份:
    1992
  • 资助金额:
    $ 18.75万
  • 项目类别:
    Grant-in-Aid for General Scientific Research (B)

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