Gas Phase Reactivity of Charged Peptide and DNA Radicals: Fundamentals and Applications

带电肽和 DNA 自由基的气相反应性：基础知识和应用

• 批准号: DP0344145
• 负责人: Prof Richard O'Hair
• 金额: $ 10.19万
• 依托单位: The University of Melbourne
• 依托单位国家: 澳大利亚
• 项目类别: Discovery Projects
• 财政年份: 2003
• 资助国家: 澳大利亚
• 起止时间: 2003-01-01 至 2005-12-31
• 项目状态: 已结题
• 来源: https://dataportal.arc.gov.au/RGS/Web/Grants/DP0344145
• 关键词: Gas; Phase; Reactivity; Charged; Peptide

Radicals derived from the "molecules of life", proteins and DNA, play both beneficial (e.g. enzyme catalysis) and deleterious roles (e.g. protein and DNA damage associated with disease). Two electrospray ionisation mass spectrometry approaches have been discovered to generate charged radicals of related models systems (e.g. peptides and nucleobases). The gas phase chemistry of these species is a largely unchartered area! We will examine the fundamental chemistry (unimolecular and bimolecular reactions) of these systems and build upon some exciting preliminary results which suggest potential applications (e.g. as a proteomics tool to sequence and distinguish between leucine and isoleucine residues in peptides).

源于“生命分子”的自由基，即蛋白质和DNA，既起到有益的作用(如酶催化作用)，又起到有害的作用(如与疾病有关的蛋白质和DNA损伤)。已发现两种电喷雾电离质谱仪方法来产生相关模型体系的带电自由基(例如多肽和碱基)。这些物种的气相化学在很大程度上是一个未知的领域！我们将研究这些系统的基本化学(单分子和双分子反应)，并建立在一些令人兴奋的初步结果的基础上，这些结果表明了潜在的应用(例如，作为蛋白质组学工具来对多肽中的亮氨酸和异亮氨酸残基进行排序和区分)。