Expression of galectin-3 and its clinical application to preoperative cytological diagnosis in thyroid neoplasms

半乳糖凝集素3的表达及其在甲状腺肿瘤术前细胞学诊断中的临床应用

• 批准号: 13670180
• 负责人: NAKANO Shizuo
• 金额: $ 0.58万
• 依托单位: Kagoshima University
• 依托单位国家: 日本
• 项目类别: Grant-in-Aid for Scientific Research (C)
• 财政年份: 2001
• 资助国家: 日本
• 起止时间: 2001 至 2002
• 项目状态: 已结题
• 来源: https://kaken.nii.ac.jp/grant/KAKENHI-PROJECT-13670180/
• 关键词: Galectin-3; Thyroid neoplasm; Preoperative diagnosis; Galectin3; ガレクチン3; 用状腺癌; 濾胞癌; 免疫染色

Galectin-3 is a member of the β-galactoside-binding protein family which plays an important role in cell-cell adhesion and in cell-matrix interaction and is expressed in many neoplastic lesion. It is reported that Galectin-3 influences to the progression and metastasis. We examined expression of galectin-3 in various thyroid neoplasms. Expression of galectin-3 was evaluated in immunohistochemically stained specimen using anti-galectin-3 antibody. A galectin-3 staining score was calculated by using stained area and concentration. Mean staining score was respectively 0.061, 0.427, and 1.514 in 69 lesions of follicular adenomas, 62 of follicular carcinomas, and 115 of papillary carcinomas. Staining score was significantly higher in the follicular carcinomas than in the follicular adenomas and was significantly higher in the papillary carcinomas than in the follicular adenomas or carcinomas.Furthermore, primary lesion of papillary carcinomas with lymph node metastases expressed higher concentration of galectin-3 than that without metastases. Expression of galectin-3 was rarely found in normal thyroid tissues. Galectin-3 expression was significantly different in 3 histological types. From these results, we assumed that galectin-3 works in different ways at different stages of thyroid neoplasm proliferation.Moreover, we examined expression of galectin-3 in a stump cytology specimen of thyroid tumor. Galectin-3 was also well expressed in cytological specimens as same as paraffin-bedded specimens. Therefore, there is a possibility that expression of galectin-3 is applicable to use for preoperative cytological diagnosis of thyroid gland tumor.

Galectin-3是β-半乳糖苷结合蛋白家族中的一员，在细胞-细胞黏附和细胞-基质相互作用中发挥重要作用，在许多肿瘤病变中都有表达。据报道，Galectin-3对肿瘤的进展和转移有影响。我们检测了Galectin-3在各种甲状腺肿瘤中的表达。用抗Galectin-3抗体进行免疫组织化学染色检测Galectin-3的表达。用染色面积和染色浓度计算Galectin-3染色积分。滤泡性腺瘤69例、滤泡性癌62例、乳头状癌115例，平均染色积分分别为0.061、0.427、1.514。滤泡性癌染色积分显著高于滤泡性腺瘤，乳头状癌显著高于滤泡性腺瘤和乳头状癌，且有淋巴结转移的乳头状癌原发灶Galectin-3表达水平高于无转移者。Galectin-3在正常甲状腺组织中很少表达。Galectin-3在3种组织学类型中的表达差异有统计学意义。根据这些结果，我们假设Galectin-3在甲状腺肿瘤增殖的不同阶段以不同的方式发挥作用。此外，我们还检测了Galectin-3在甲状腺肿瘤残端细胞学标本中的表达。与石蜡包埋标本一样，Galectin-3在细胞学标本中也有较好的表达。因此，Galectin-3的表达有可能用于甲状腺肿瘤的术前细胞学诊断。