Histological investigation of information pathway of antigen by hypermulticolor fluorescence imaging

超彩色荧光成像对抗原信息通路的组织学研究

• 批准号: 13670227
• 负责人: TSURUI Hiromichi
• 金额: $ 2.3万
• 依托单位: Juntendo University
• 依托单位国家: 日本
• 项目类别: Grant-in-Aid for Scientific Research (C)
• 财政年份: 2001
• 资助国家: 日本
• 起止时间: 2001 至 2002
• 项目状态: 已结题
• 来源: https://kaken.nii.ac.jp/grant/KAKENHI-PROJECT-13670227/
• 关键词: lymphocyte; dendritic cell; macrophage; trafficking; hypermulticolor imaging; white pulp; 画像分光; 超多色免疫蛍光染色; マクロファージ; 樹状細胞; 獲得免疫; 自己寛容; Dendritic cell; Toll like receptor; 自然免疫; Th1; Th2分化; GFP; hyper-spectral imaging

To obtain the original purpose of the project, present knowledge concerning the 3 dimensional structure of mouse spleen was quite limited, so, I had to clarify the 3 dimensional structure of mouse spleen, especially in terms with the vascular system.The summary of the findings obtained during this year is as follows;1.Based on SEM images, there are the vascular systems that correspond to artery and vein. However, they showed unusual vascular system, namely, bundled tubular structure and diverged into thin cords with their characteristic configuration, resulted in a loose meshwork. Not only the central artery, but "funnel shaped" artery also flowed into the white pulp. Another type of vascular system corresponding vein stemed from white pulp.2.The findings obtained by fluorescence histochemistry well coincided with that of SEM. Lymphocyte, dendritic cells and macrophages, located in the wall of thick artery, were prominent. It suggests those cells transfer to the white pulp from those thick arteries through the red pulp.3.When LPS was loaded, some dendritic cells transfer to PALS through the central artery.4.The precursor of marginal metallophilic macrophage escaped from both thick trunk artery and middle artery in the red pulp, form a cluster and transfered to the boundary of marginal zone and follicle.

为了达到这个项目的最初目的，目前对小鼠脾脏三维结构的了解还相当有限，因此，我必须澄清小鼠脾脏的三维结构，特别是血管系统。这一年来所获得的结果总结如下：1.基于SEM图像，有对应于动脉和静脉的血管系统。然而，它们的血管系统异常，即束管状结构，并分叉成细线，其特征性的配置，导致一个松散的网络。除中央动脉外，“漏斗状”动脉也流入白色髓。白色髓中有另一种类型的血管系统，与静脉相对应。2.荧光组织化学观察结果与扫描电镜观察结果一致。淋巴细胞、树突状细胞和巨噬细胞分布于粗动脉壁。3. LPS负荷后，部分树突状细胞通过中央动脉转移至帕尔斯; 4.边缘巨噬细胞前体从红髓中的粗干动脉和中动脉中逃逸，聚集并转移至边缘区和滤泡的交界处。

项目成果

Yan Xiu, et al.: "Transcriptional regulation of Fcgr2b gene by polymorphic promoter region and Its contribution to humoral Immune response"J.Immunology. 169. 4340-4346 (2002)

严秀等：“多态性启动子区对Fcgr2b基因的转录调控及其对体液免疫反应的贡献”，免疫学杂志。

Li N, Nakamura K, Jiang Y, Tsurui H, Matsuoka S, Abe M, Ohtsuji M, Nishimura H, Kato K, Kawai T, Atsumi T, Koike T, Shirai T, Ueno H, Hirose S.: "Gain-of-function polymorphism in mouse and human Ltk : implications for the pathogenesis of systemic lupus er

Li N、Nakamura K、Jiang Y、Tsurui H、Matsuoka S、Abe M、Ohtsuji M、Nishimura H、Kato K、Kawai T、Atsumi T、Koike T、Shirai T、Ueno H、Hirose S.：“Gain-of

Li N, Nakamura K, Jiang Y, Tsurui H,......., Hirose S.: "Gain-of-function polymorphism in mouse and human Ltk : implications for the pathogenesis of systemic lupus erythematosus"Humam Molecular Genetics. 15. 171-179 (2004)

Li N、Nakamura K、Jiang Y、Tsurui H、.......、Hirose S.：“小鼠和人类 Ltk 中的功能多态性获得：对系统性红斑狼疮发病机制的影响”Humam 分子遗传学。

Matsuoka S, Tsurui H,......, Shirai T: "A monoclonal antibody to the alpha2 domain of murine major histocompatibility complex class I that specifically kills activated lymphocytes and blocks liver damage in the concanavalin A hepatitis model"Journal of Ex

Matsuoka S、Tsurui H、......、Shirai T：“一种针对鼠主要组织相容性复合物 I 类 alpha2 结构域的单克隆抗体，可特异性杀死伴刀豆球蛋白 A 型肝炎模型中的活化淋巴细胞并阻断肝损伤”Journal of Ex

Xiu Y, Nakamura K, Abe M, Li N, Wen XS, Jiang Y, Zhang D, Tsurui H,....., Hirose S: "Transcriptional Regulation of Fcgr2b Gene by Polymorphic Promoter Region and Its Contribution to Humoral Immune Responses"Journal of Immunology. 169. 4340-4346 (2002)

Xiu Y，Nakamura K，Abe M，Li N，Wen XS，Jiang Y，Zhang D，Tsurui H，.....，Hirose S：“多态性启动子区域对 Fcgr2b 基因的转录调控及其对体液免疫反应的贡献