Histological investigation of information pathway of antigen by hypermulticolor fluorescence imaging

为了达到项目的初衷，目前对小鼠脾的三维结构的了解相当有限，因此，我不得不澄清小鼠脾的三维结构，特别是与血管系统有关的三维结构。总结这一年的发现如下：1.根据扫描电子显微镜图像，有对应于动脉和静脉的血管系统。然而，它们表现出异常的维管系统，即束状管状结构，并以其特有的形态分叉成细索，导致网状结构疏松。不仅是中央动脉，“漏斗状”动脉也流入白色牙髓。另一种类型的血管系统对应的静脉来自白髓。2.荧光组织化学的结果与扫描电子显微镜的结果很好地吻合。淋巴细胞、树突状细胞和巨噬细胞分布于大动脉壁。提示这些细胞通过红髓从粗大的动脉向白髓转移。3.当负载脂多糖时，部分树突状细胞通过中央动脉向PALS转移。4.边缘嗜金属巨噬细胞前体从红髓的粗大动脉和中间动脉逃逸，形成一簇，转移到边缘带和毛囊的边界。

To obtain the original purpose of the project, present knowledge concerning the 3 dimensional structure of mouse spleen was quite limited, so, I had to clarify the 3 dimensional structure of mouse spleen, especially in terms with the vascular system.The summary of the findings obtained during this year is as follows;1.Based on SEM images, there are the vascular systems that correspond to artery and vein. However, they showed unusual vascular system, namely, bundled tubular structure and diverged into thin cords with their characteristic configuration, resulted in a loose meshwork. Not only the central artery, but "funnel shaped" artery also flowed into the white pulp. Another type of vascular system corresponding vein stemed from white pulp.2.The findings obtained by fluorescence histochemistry well coincided with that of SEM. Lymphocyte, dendritic cells and macrophages, located in the wall of thick artery, were prominent. It suggests those cells transfer to the white pulp from those thick arteries through the red pulp.3.When LPS was loaded, some dendritic cells transfer to PALS through the central artery.4.The precursor of marginal metallophilic macrophage escaped from both thick trunk artery and middle artery in the red pulp, form a cluster and transfered to the boundary of marginal zone and follicle.