Histological analysis of T cell differentiation process based on hypermulticolor-hypersensitive fluorescence imaging method

基于超多色-超灵敏荧光成像方法的T细胞分化过程的组织学分析

• 批准号: 22590194
• 负责人: TSURUI Hiromichi
• 金额: $ 2.83万
• 依托单位: Juntendo University
• 依托单位国家: 日本
• 项目类别: Grant-in-Aid for Scientific Research (C)
• 财政年份: 2010
• 资助国家: 日本
• 起止时间: 2010 至 2012
• 项目状态: 已结题
• 来源: https://kaken.nii.ac.jp/grant/KAKENHI-PROJECT-22590194/
• 关键词: 画像分光; 半導体色素; サイトカインネットワーク; 超高感度蛍光イメージング; 分化特異的転写因子; 細胞分化・組織形成; 蛍光免疫組織染色; 超多色蛍光イメージング; サイトカイン染色; cytokine; multi agent system; molecular dynamics; GPGPU; MM-PBSA; TCR-pMHC相互作用; T細胞分化; 蛍光イメージング

As the methods to evaluate chromophore-antibody conjugation state, gel-filtration chromatography, fluorescence-correlation spectroscopy, hyper-sensitive spectral imaging and high-magnification SEM were established or applied. Through those methods, two conjugation methods were established and applied to simultaneous imaging of T -bet, GATA-3, ROR-gt, FoxP3. IL-10 and IFN-a were also successfully imaged.

作为评价发色团-抗体结合状态的方法，建立或应用了凝胶过滤色谱、荧光相关光谱、超灵敏光谱成像和高倍扫描电镜。通过这些方法，建立了两种偶联方法，并应用于T-bet、加塔-3、ROR-gt、FoxP 3的同时成像。IL-10和IFN-α也成功成像。

项目成果

Phenotype conversion from RA to SLE in FcγRIIB-deficient B6 mice by Yaa mutation

Yaa 突变导致 FcγRIIB 缺陷 B6 小鼠从 RA 向 SLE 的表型转变

• 发表时间: 2012
• 作者: KAWANO Shinya;AMANO Hirofumi;LIN Qingshun;KANEKO Toshiyuki;NISHIKAWA Keiko;OKAZAKI Hideki;TSURUI Hiromichi;NISHIMURA Hiroyuki;SHIRAI Toshikazu;TAKASAKI Yoshinari;HIROSE Sachiko.
• 通讯作者: HIROSE Sachiko.

Practical Estimation of TCR-pMHC binding free-energy based on the dielectric model and the Coarse-grained model in “Molecular Dynamics - Studies of Synthetic and Biological Macromolecules”

《分子动力学-合成与生物大分子的研究》中基于介电模型和粗粒度模型的TCR-pMHC结合自由能的实用估计

• 发表时间: 2012
• 作者: 全〓坤;前田誠司;大谷(桑原)佐知;田中宏一;早川徹;関真;Horiguchi K et al.;Tsurui Hiromichi and Takahashi Takuya
• 通讯作者: Tsurui Hiromichi and Takahashi Takuya

FTY720 exerts a survival advantage through the prevention of end-stage glomerular inflammation in lupus-prone BXSB mice

• DOI: 10.1016/j.bbrc.2010.03.078
• 发表时间: 2010-04-09
• 期刊: BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS
• 影响因子: 3.1
• 作者: Ando, Seiichiro;Amano, Hirofumi;Takasaki, Yoshinari
• 通讯作者: Takasaki, Yoshinari

Phagocytosis specific autofluorescence in marginal metallophilic macrophage and marginal zone macrophage

边缘嗜金属巨噬细胞和边缘区巨噬细胞的吞噬作用特异性自发荧光

• 发表时间: 2010
• 期刊: International Immunology
• 影响因子: 4.4
• 作者: Tsurui H;Hirose S
• 通讯作者: Hirose S

A novel locus of B6 mice on chromosome 12 plays a role in common process shared by SLE, RA, and Sjögren syndrome

B6 小鼠 12 号染色体上的一个新基因座在 SLE、RA 和干燥综合征共有的共同过程中发挥作用

• 发表时间: 2012
• 作者: Kaneko Toshiyuki;AMANO Hirofumi;OHTSUJI Mareki;NISHIKAWA K;KAWANO S;OHTSUJI N;Lin Q;OKAZAKI H;TSURUI H;NISHIMURA H;SHIRAI T;TAKASAKI Y;HIROSE Sachiko.
• 通讯作者: HIROSE Sachiko.