Experimental study of generation mechanism of thinner abuser's sudden death: Molecular biological examination of myocardial damage

fhinner abouser突然死亡的原因可能还不确定。观察甲苯吸入对心脏器官的影响。雄性Wistar大鼠吸入甲苯（1，500 ppm，每天4小时）7天。病理检查心脏未见明显异常，如冠状动脉闭塞、狭窄等。冠状动脉内血管壁、内皮和狭窄异常清晰。未见心肌排列紊乱，间质未见细胞浸润。即使通过传导系统也没有观察到明显的异常，如细胞侵入。然后，对氧化损伤的化学标记物进行染色。DNA的氧化损伤，如8-OH-Dg，已被承认，但脂质过氧化物，如4-HNE的形成尚不清楚。SOD免疫组化染色显示吸入组小血管呈免疫阳性反应。关于这一结果，认为血液SOD循环向心脏的供应（达到）是SOD出现的原因。通过8- OH-Dg定量分析，其为1.150[+！-]对照组为0.096，1.073[+！-] 0.119 ng/ml。该结果与免疫组化染色结果一致。因此，氧化性DNE损伤的产生无法定量证实。提出了小容器中SOD的出现起抗氧化作用，从而抑制甲苯对8-OH-Dg的显色的可能性。过氧化损伤是由甲苯直接引起的，或甲苯吸入过量产生促肾上腺皮质激素引起心脏过氧化损伤的可能性。结果提示，该药物可能影响心功能。

The cause of fhinner abouser's sudden death might be uncertain. The effect of toluene inhalation on heart organs was examined. Male Wistar rats was inhaled toluene (1,500 ppm for 4 hours a day) for 7 days. Pathologically the heart was observed, therefor there was no clear anomalous such as the obturations and the strictures in the coronary aretery. Anomalous clear as for the vessel wall and the endothelial and the strictures in the coronary artery. The myocardial disarray was not observed, and the cell infiltration was not observed in the interstitum. Clear anomalous such as cell invasions was not observed even by the conduction system either. Then, immunohistochemically markers of oxidative damage were stained. The oxidative DNA damage, such as 8-OH-Dg was admitted, although the formation of lipidperoxide, such as 4-HNE was not clear. The immunohistochemical staining of SOD revealed that immunopositivity in the small vessels of the inhalation group. As for this result, the supply of circulation of the blood SOD (attainment) to the heart was thought to the appearance of SOD. By quantitative analysis of 8- OH-Dg, it was 1.150【+!-】0.096 in the control and 1.073【+!-】0.119 in ng/ml in treated group. This result was corresponding to the immunohistochemical staining result well. Therefore, the generation of the oxidative DNE injury was not able to be confirmed quantitatively. The possibility that the appearance accentuation of 8-OH-Dg by the toluene is controlled since the appearance of SOD in the small vessel acted anti-oxidativelly was suggested. The peroxidative damage is caused to the heart directly by the toluene, or the possibility to couse the heart the peroxidation damage through an excessive production of the adrencorticotropine by the toluene inhalation is suggestion. As a result, the possibility to influence the cardiac function was suggested.