Physiological role of human specific seminal ubiquitinated CD10-CD13 complex enzyme

人精液泛素化CD10-CD13复合酶的生理作用

• 批准号: 13670431
• 负责人: ABE Sumiko
• 金额: $ 0.58万
• 依托单位: FUKUSHIMA MEDICAL UNIVERSITY
• 依托单位国家: 日本
• 项目类别: Grant-in-Aid for Scientific Research (C)
• 财政年份: 2001
• 资助国家: 日本
• 起止时间: 2001 至 2002
• 项目状态: 已结题
• 来源: https://kaken.nii.ac.jp/grant/KAKENHI-PROJECT-13670431/
• 关键词: CD10-CD13 complex; γ-glutamyl transpeptidase active enzyme; human seman; HL60; HeLa; mRNA; MBL

Human semen-specific γ-glutamyl transpeptidase active protein (SEGAP) is a unique enzyme complex of ubiquitinated CD10 and CD13. In our previous study, we examined the potential of SEGAP to induce phosphorylation of tyrosine in HeLa (human cervical carcinoma) and HL60 (human promyelocytic leukemia) cells, and we observed phosphorylation of 110-kDa, 70-kDa and 60-kDa proteins that are thought to be PI3 kinase, Syk/ZAP-70 and Scr family proteins, respectively. These results suggested that SEGAP affects mRNA expressions of cytokines and other signals in HeLa and HL60 cells.In this study, we investigated the changes in mRNA levels in SEGAP-treated HeLa and HL60 cells. IL-1β, IL-8, IL-12, TNF-a, Lyn, Fas and c-fos mRNAs were all expressed in HeLa cells, and their expression levels were not affected by SEGAP. Mannose-binding lectin (MBL) mRNAs were not expressed in control HeLa cells. On the other hand, SEGAP induced MBL mRNA expression in HeLa cells after 15 to 30 min of incubation. Since MBL initiates the lectin complement pathway that mediates the innate immune system, SEGAP may activate the defense system against invading microorganisms. SEGAP did not affect mRNA levels of IL-1β, IL-8, IL-12, TNF-a, Lyn or c-fos in HL60 cells. CT-1 and iNOS mRNAs were not expressed in control HL60 cells. In a manner similar to that in HeLa cells, SEGAP induced CT-1 and iNOS mRNA expressions in HL60 cells after 15 to 30 min of incubation. NO is one of the messenger molecules involved in the bacteria killing process. These results suggest that SEGAP induces lectin, CT-1 and iNOS mRNA expressions in order to protect sperm from invading microorganisms.

人精液特异性γ-谷氨酰转肽酶活性蛋白（SEGAP）是一种独特的泛素化CD 10和CD 13的酶复合物。在我们先前的研究中，我们检测了SEGAP诱导HeLa（人宫颈癌）和HL 60（人早幼粒细胞白血病）细胞中酪氨酸磷酸化的潜力，并且我们观察到分别被认为是PI 3激酶、Syk/ZAP-70和Scr家族蛋白的110-kDa、70-kDa和60-kDa蛋白的磷酸化。这些结果表明，SEGAP影响HeLa和HL 60细胞的细胞因子和其他信号的mRNA表达。IL-1β、IL-8、IL-12、TNF-α、林恩、Fas和c-fos mRNA在HeLa细胞中均有表达，且其表达水平不受SEGAP的影响。甘露糖结合凝集素（MBL）mRNA在对照HeLa细胞中不表达。另一方面，SEGAP在孵育15至30分钟后诱导HeLa细胞中的MBL mRNA表达。由于MBL启动介导先天免疫系统的凝集素补体途径，因此SEGAP可以激活针对入侵微生物的防御系统。SEGAP对HL 60细胞IL-1β、IL-8、IL-12、TNF-α、林恩和c-fos的mRNA水平无影响。对照组HL 60细胞不表达CT-1和iNOSmRNA。在HeLa细胞中，SEGAP以类似的方式诱导HL 60细胞中的CT-1和iNOSmRNA表达在孵育15至30分钟后。NO是参与细菌杀伤过程的信使分子之一。这些结果表明，SEGAP诱导凝集素，CT-1和iNOS mRNA的表达，以保护精子免受入侵的微生物。

项目成果

Abe S., et al.: "Cytokine expression in blood leukocytes of a rat after application of a tourniuet to a hind llimb"5^<th> International Symposium on Advances in Legal Medicine Abstracts. 115 (2002)

Abe S.等人：“对后肢应用止血带后大鼠血液白细胞中的细胞因子表达”第五届国际法律医学摘要进展研讨会。

Sumiko Abe, Ikubumi Mizusawa, Katsuyuki Kanno, Atsuko Yabashi, Takashi Honda, Miwako Suto, Mikio Kuraya, Kouichi Hiraiwa: "Changes in mRNA expression patterns for cytokines in blood leukocytes of a rat tourniquet model"Legal Medicine. in press.

Sumiko Abe、Ikubumi Mizusawa、Katsuyuki Kanno、Atsuko Yabashi、Takashi Honda、Miwako Suto、Mikio Kuraya、Kouichi Hiraiwa：“大鼠止血带模型血液白细胞中细胞因子 mRNA 表达模式的变化”法律医学。

阿部すみ子: "前立腺病変による精液特異 γ-glutamyl transpeptidase活性酵素構成ユビキチンの免疫反応性の変化"福島医学雑誌. 51・3. 189-197 (2001)

Sumiko Abe：“前列腺病变引起的精液特异性γ-谷氨酰转肽酶活性酶成分泛素的免疫反应性变化”福岛医学杂志51・3（2001）。

Sumiko Abe, Miwako Suto, Osamu Muraki, Mikio Kuraya, Teizo Fujita, Masahide Nakamura, Hirobumi Gunji, Kouichi Hiraiwa: "Immunohistochemical characterization of seminal γ-glutamyl transpeptidase active protein constituent-ubiquitin in prostate disease"Fuku

Sumiko Abe、Miwako Suto、Osamu Muraki、Mikio Kuraya、Teizo Fujita、Masahide Nakamura、Hirobumi Gunji、Kouichi Hiraiwa：“前列腺疾病中精液γ-谷氨酰转肽酶活性蛋白成分-泛素的免疫组织化学特征”Fuku

Abe S., et al.: "NOS mRNA expression in a rat dorsal root ganglion after application of a tourniquet to a hind limb"Journal of Forensic Medicine (16^<th> Meeting of the International Association of Forensic Sciences Abstract book). 45. 96 (2002)

Abe S.等人：“对后肢应用止血带后大鼠背根神经节中NOS mRNA的表达”法医学杂志（国际法医学协会第16次会议摘要书）。