A novel transcriptional factor ZP140 as an autoantigen reactive with sera from patient with Sjogren's syndrome

一种新型转录因子 ZP140 作为自身抗原，与干燥综合征患者的血清发生反应

• 批准号: 13670446
• 负责人: NOJIMA Yoshihisa
• 金额: $ 2.62万
• 依托单位: Gunma University School of Medicine
• 依托单位国家: 日本
• 项目类别: Grant-in-Aid for Scientific Research (C)
• 财政年份: 2001
• 资助国家: 日本
• 起止时间: 2001 至 2002
• 项目状态: 已结题
• 来源: https://kaken.nii.ac.jp/grant/KAKENHI-PROJECT-13670446/
• 关键词: autoimmune disease; Sogren's syndrome; transcriptional factor; 自己抗体

Autoantibodies found in patients with a variety of autoimmune diseases provide valuable tools not only to clarify disease mechanisms but also to identify and characterize structure and function of target antigens. By using sera from patients with Sjogren's syndrome as a probe, we isolated cDNA encoding a novel protein from human testing cDNA library. Sequence analysis revealed that the protein, designated ZP140, had putative several functional motifs including signal for nuclear localization, Knuppel type Zn finger, PHD domain, prolin-rich domain, and seven SH3-binding domains. These structural characteristics strongly suggest that ZP140 is a novel transcriptional factor. The autoantibody reactive with ZP140 is specific for Sjogren's syndrome. Thus, further characterization of ZP140 may lead to understanding of pathogenesis of this systemic autoimmune disease.

在各种自身免疫性疾病患者中发现的自身抗体不仅为阐明疾病机制，而且为鉴定和表征靶抗原的结构和功能提供了有价值的工具。以干燥综合征患者血清为探针，从人检测cDNA文库中分离到编码新蛋白的cDNA。序列分析表明，该蛋白被命名为ZP140，具有核定位信号、Knuppel型锌指、PHD结构域、富含脯氨酸结构域和7个sh3结合结构域等功能基序。这些结构特征强烈提示ZP140是一种新的转录因子。与ZP140反应的自身抗体是干燥综合征的特异性抗体。因此，进一步表征ZP140可能有助于了解这种系统性自身免疫性疾病的发病机制。

项目成果

Tsukada Y, Toh BH: "Novel variant of p230 trans-Golgi metwork protein Identified by serum from Sjogren's syndrome patient"Eur J Cell Biol. 79(11). 790-794 (2000)

Tsukada Y、Toh BH：“由干燥综合征患者血清鉴定出的 p230 反式高尔基体工作蛋白的新变体”Eur J Cell Biol。

Tsukada Yoshito: "Autoantibody to DNA excision repair enzyme hMYH in a patient with rheumatic disease"Clin Immunol. 99. 291-297 (2001)

Tsukada Yoshito：“风湿病患者体内 DNA 切除修复酶 hMYH 的自身抗体”Clin Nutritionol。

Ota F, Maeshima A, Yamashita S, Ikeuchi H, Kaneko Y, Kuroiwa T, Hiromura K, Ueki K, Kojima I, Nojima Y: "Activin A is an inducer for proliferation of fibroblast-like synoviocytes of rheumatoid arthritis"Arthritis Rheum. (in press). (2003)

Ota F、Maeshima A、Yamashita S、Ikeuchi H、Kaneko Y、Kuroiwa T、Hiromura K、Ueki K、Kojima I、Nojima Y：“激活素 A 是类风湿关节炎成纤维样滑膜细胞增殖的诱导剂”关节炎大黄。

Ota F, Maeshima A, Yamashita S, Ikeuchj H, Kaneko Y, Kuroiwa T, Hiromura K, Ueki K, Kojima I, Nojima Y.: "Activin A is an inducer for proliferation of fibroblast-like synoviocytes of rheumatoid arthritis."Arthritis Rheum. in press. (2003)

Ota F、Maeshima A、Yamashita S、Ikeuchj H、Kaneko Y、Kuroiwa T、Hiromura K、Ueki K、Kojima I、Nojima Y.：“激活素 A 是类风湿关节炎成纤维样滑膜细胞增殖的诱导剂。”关节炎

Lai EP, Tsukada Y, Tob BH: "Autoantibody to DNA excision repair enzyme hMYH in a patient withrheumatic disease"Clin Immunol. 99(2). 291-297 (2001)

Lai EP、Tsukada Y、Tob BH：“风湿病患者体内针对 DNA 切除修复酶 hMYH 的自身抗体”Clin Immunol。