Gene transfer to hematopoietic stem cells by a newly-designed retroviral vectors and expression of transgenes in the liver

点击翻译按钮获取中文摘要

We equipped the FMEV type retrovirus rector with HBV enhancer motif and CAT gene. Then, the HCC cell lines, Huh7, Alexander, HLE were infected with the vector. The expression efficiency of the transduced marker genes were increased by 1.5 to 2.0 times higher than the control vector. We also checked the usefulness of the newly-designed retroviral vector in mice in vivoWe equipped the FMEV type retroviral vector with a kind of insulator "URI", then the cell lines, K562, Mam, NIH3T3, 293 were infected with the vector. The expression efficiency of the transduced marker genes were increased by 1.2 to 1.5 times higher than the control vector. We are now investigating the relationship between methylation and silencing of the transded genes in the vectorWe isolated bone marrow cells from the C57BL/6-GFP mice and infected the cells with the control or newly-desined FMEV vetor. The evaluation of GFP positive cells as well as the expression efficiency of the transduced genes in the liver and peripheral blood are under investigation