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Homeostasis of gastric mucosa and effects of peptidergic nerve on mucus metabolism

胃粘膜稳态及肽能神经对粘液代谢的影响

基本信息

批准号：
13670554
负责人：
ICHIKAWA Takafumi
金额：
$ 1.02万
依托单位：
Kitasato University
依托单位国家：
日本
项目类别：
Grant-in-Aid for Scientific Research (C)
财政年份：
2001
资助国家：
日本
起止时间：
2001 至 2002
项目状态：
已结题

项目摘要

In this study, we examined the maintenance of gastric mucosa and the role of peptidergic nerve on epithelium.1) Our histochemical findings indicate that immunoreactivity for neuropeptides including CGRP distributes in the nerve fibers of the lamina propria and submucosal layer of rat gastric mucosa. Although CGRP-immunoreactive nerve fibers mainly innervate the submucosal microvasculature, a certain part of these fibers is shown to run parallel and close to the gastric glands in the lamina propria, some of which approach the surface epithelial cells of the rat stomach.2) Radiolabeled mucin was obtained from the tissue of the rat stomachs incubated for 5 h with [3H]glucosamine. The incorporation of [3H]glucosamine into the mucin was stimulated by CGRP in the cultured corpus mucosa.3) Detailed research on the healing process of gastric mucosa from injury due to various necrotizing agents is important for homeostasis of gastric mucosa. To elucidate this issue, we prepared monoclonal antibodies reacting with mucin molecules present in the specific region and layer of the gastrointestinal mucosa. Acetic acid-induced gastric ulcers were prepared in Wistar rats. Following 24-h fasting, the animals were killed at 20, 30 and 50 days after acid insult and their stomachs were removed immediately. A notable but temporary expression of a kind of sialomucin specifically stained with HCM31 was observed in the regenerating epithelia during the healing stage of acetic acid-induced gastric damage.4) These findings suggest that CGRP acts on the corpus surface epithelial cells and participates in the maintenance of the rat gastric mucosa.

本实验研究了胃粘膜的维持和肽能神经在胃粘膜上皮中的作用。1）组织化学结果表明，CGRP等神经肽的免疫反应分布于胃粘膜固有层和粘膜下层的神经纤维中。虽然CGRP免疫反应阳性神经纤维主要支配粘膜下微血管，但这些纤维中的一部分在固有层中与胃腺平行并接近，其中一些接近大鼠胃的表面上皮细胞。CGRP可刺激培养的胃粘膜中[~ 3 H]氨基葡萄糖掺入到粘蛋白中。3）深入研究各种坏死因子引起的胃粘膜损伤的愈合过程对胃粘膜的自稳具有重要意义。为了阐明这一问题，我们制备了与存在于胃肠道粘膜的特定区域和层中的粘蛋白分子反应的单克隆抗体。在Wistar大鼠中制备乙酸诱导的胃溃疡。禁食24小时后，在酸损伤后20、30和50天处死动物，立即切除胃。在乙酸诱导的胃损伤愈合期，再生上皮细胞中有一种HCM 31特异性的唾液粘蛋白的表达，这种表达是暂时性的。4）提示CGRP作用于胃体表面上皮细胞，参与胃粘膜的维持。