The newly born baby/infant type pepsinogen It's regulation mechanism and the potential of Onco-protein

新生婴儿/婴儿型胃蛋白酶原的调控机制及Onco蛋白的潜力

• 批准号: 13670548
• 负责人: KITAUCHI Shintaro
• 金额: $ 1.92万
• 依托单位: Wakayama Medical University
• 依托单位国家: 日本
• 项目类别: Grant-in-Aid for Scientific Research (C)
• 财政年份: 2001
• 资助国家: 日本
• 起止时间: 2001 至 2002
• 项目状态: 已结题
• 来源: https://kaken.nii.ac.jp/grant/KAKENHI-PROJECT-13670548/
• 关键词: Gastric mucosa; Pepsinogen; Development; Differentiation; Growth; Structure formation; 骨粘膜

1)Based on the results of the protein primary structures of the newly born baby/infant type pepsinogen (PGF) and chymosin (CHY) identified by our cDNA cloning, synthetic peptides were prepared to immunize rabbits and 2 kinds of antibodies were obtained against PGF Immunohistochemistry revealed that PGF appeared on Day 16 of rat fetuses and the producing cells would, at least in part, constitute a gastric gland in the future.2)We have been performing genomic cloning of these genes with the rat genomic library previously established using the PGF cDNA we cloned. To date, we have examined 41 positive clones, but all of them were clones for PGC and we have not obtained genomic clones for PGF.3)We extracted RNA and protein soluble fractions from gastric mucosal epithelial tissue of rat fetuses using the separation method of the gastrointestinal epithelium that we developed. In the later fetal period, from the morphology formation period of the stomach to birth, we investigated the expressio … More n of PGF CHY, and adult type pepsinogen (PGA) at the gene levels (by quantitative PCR) and protein levels (by zymography). As a result, the PGF gene expression first appeared on Day 15 in the fetal stomach, and later the expression of the CHY gene appeared. The expression of the PGF gene was mainly observed in the period of morphological formation of the stomach and a trace of PGA expression could be found. Just before birth, the CHY gene was upregulated to exceed the expression of PGF, and in the weaning period, in turn, the PGA expression exceeded the CHY expression.4)Using the in vivo experiment system on newly born rats, we found that the expression of the pepsinogen genes switched from PGF to PGA by stimulating the rats with differentiation-inducing factors on the gastric mucosa, such as adrenal corticosteroids. Using the primary culture system of fetal gastric epithelium that we developed, we confirmed that the gene expression switched from PGF or CHY to PGA by administering differentiation-inducing factors on gastric epithelial cells, such as steroids and dibutyl-cAMP. Less

1)根据我们克隆的新生婴儿型胃蛋白酶原（PGF）和凝乳酶（CHY）的蛋白质一级结构的结果，制备了合成肽免疫家兔，获得了两种抗PGF的抗体。免疫组化显示，PGF在大鼠胎龄16天出现，2）我们已经用我们克隆的PGF cDNA建立的大鼠基因组文库进行了这些基因的基因组克隆。到目前为止，我们已经检测了41个阳性克隆，但它们都是PGC的克隆，我们还没有获得PGF的基因组克隆。3）我们使用我们开发的胃肠上皮分离方法从大鼠胎胃粘膜上皮组织中提取RNA和蛋白质可溶性组分。在胎儿后期，从胃的形态形成期到出生，我们研究了胃的表达， ...更多信息 在基因水平（通过定量PCR）和蛋白水平（通过酶谱法）测定PGF CHY和成人型胃蛋白酶原（PGA）的n。结果，PGF基因表达首先出现在第15天的胎胃中，随后出现CHY基因的表达。PGF基因的表达主要在胃形态形成期，有少量PGA表达，在出生前CHY基因表达上调，超过PGF的表达，在断奶期PGA表达又超过CHY的表达。4）利用新生大鼠体内实验系统，我们发现，胃蛋白酶原基因的表达由PGF转换为PGA，通过刺激大鼠胃粘膜上的分化诱导因子，如肾上腺皮质类固醇。使用我们开发的胎儿胃上皮的原代培养系统，我们证实了通过对胃上皮细胞施用分化诱导因子，如类固醇和二丁基-cAMP，基因表达从PGF或CHY切换到PGA。少

项目成果

Nakata H, Ichinose M.: "Helicobacter pylori and iron deficiency anemia"Intern Med.. 42(10). 922-923 (2003)

Nakata H，Ichinose M.：“幽门螺杆菌和缺铁性贫血”Intern Med.. 42(10)。

A.Tateishi: "Extensive mesenteric vein and portal vein thrombosis successfully treated thrombolysis and anticoagulation"J Gastroenterol Hepatol. 16. 1426-1433 (2002)

A.Tateishi：“广泛的肠系膜静脉和门静脉血栓成功地治疗了溶栓和抗凝”J Gastroenterol Hepatol。

A.Shiotani: "Association between gastric myoelectrical activity and intraluminal nitric oxide"Aliment Pharmacol Ther. 16. 44-51 (2002)

A.Shiotani：“胃肌电活动与腔内一氧化氮之间的关联”Aliment Pharmacol Ther。

M.Fujishiro: "Endoscopic spraying of sucralfate using the Outer sheath of a clipping device"Endoscopy. 34. 935-935 (2002)

M.Fujishiro：“使用修剪装置的外鞘内窥镜喷雾硫糖铝”内窥镜检查。

一瀬 雅夫: "座談会 Helicobacter pylori除菌時代を迎えた胃疾患診療の現状"内科. 89. 507-519 (2002)

Masao Ichinose：“圆桌讨论：根除幽门螺杆菌时代胃病治疗的现状”《内科》89. 507-519 (2002)。