Study on function of cytokine LECT2 expressed in the liver using a mouse
利用小鼠肝脏表达的细胞因子LECT2的功能研究
基本信息
- 批准号:13670581
- 负责人:
- 金额:$ 2.37万
- 依托单位:
- 依托单位国家:日本
- 项目类别:Grant-in-Aid for Scientific Research (C)
- 财政年份:2001
- 资助国家:日本
- 起止时间:2001 至 2003
- 项目状态:已结题
- 来源:
- 关键词:
项目摘要
LECT2 (leukocyte cell-derived chemotaxin 2) was originally identified for its possible chemotactic activity against human neutrophils in vitro. It is a 16-kDa protein that is preferentially expressed in the liver. Its homologues have been widely identified in many vertebrates. Current evidence suggests that LECT2 may be a multifunctional protein like cytokines. However, the function of LECT2 in vivo remains unclear. To elucidate the role of this protein in vivo, we have generated LECT2-deficient (LECT2^<-/->) mice. We found that the proportion of natural killer T (NKT) cells in the liver increased significantly in LECT2^<-/-> mice, although those of conventional T cells, NK cells, and other cell types were comparable with those in wild-type mice. Consistent with increased hepatic NKT cell number, production of IL-4 and IFN-_Y was augmented in LECT2^<-/-> mice upon stimulation with α-galactosylceramide (α-GalCer), which specifically activates Vα14^+ NKT cells. In addition, NKT cell-mediated cytotoxic activity against syngeneic thymocytes also increased in hepatic mononuclear cells obtained from LECT2^<-/-> mice in vitro. Interestingly, the hepatic injury was exacerbated in LECT2^<-/-> mice upon treatment with Con A, possibly because of the significantly higher expression of IL-4 and Fas ligand. These results suggest that LECT2 might regulate the homeostasis of NKT cells in the liver, and might be involved in the pathogenesis of hepatitis.
LECT2(白细胞来源的趋化因子 2)最初因其体外可能对人中性粒细胞的趋化活性而被鉴定。它是一种 16 kDa 蛋白质,优先在肝脏中表达。其同源物已在许多脊椎动物中得到广泛鉴定。目前的证据表明,LECT2 可能是一种类似于细胞因子的多功能蛋白。然而,LECT2在体内的功能仍不清楚。为了阐明这种蛋白质在体内的作用,我们培育了 LECT2 缺陷型 (LECT2^-/->) 小鼠。我们发现,LECT2^-/-> 小鼠肝脏中自然杀伤 T (NKT) 细胞的比例显着增加,尽管常规 T 细胞、NK 细胞和其他细胞类型的比例与野生型小鼠相当。与肝脏 NKT 细胞数量增加一致,LECT2^-/- 小鼠在用 α-半乳糖神经酰胺 (α-GalCer) 刺激后,IL-4 和 IFN-_Y 的产生增加,α-GalCer 特异性激活 Vα14^+ NKT 细胞。此外,在体外从LECT2^-/-小鼠获得的肝单核细胞中,NKT细胞介导的针对同基因胸腺细胞的细胞毒活性也增加。有趣的是,用Con A治疗后,LECT2^-/-小鼠的肝损伤加剧,可能是因为IL-4和Fas配体的表达显着升高。这些结果表明LECT2可能调节肝脏NKT细胞的稳态,并可能参与肝炎的发病机制。
项目成果
期刊论文数量(16)
专著数量(0)
科研奖励数量(0)
会议论文数量(0)
专利数量(0)
Ohashi YY, et al.: "Novel missense mutation found in a Japanese patient with myeloperoxidase deficiency."Gene. 327(2). 195-200 (2004)
Ohashi YY 等人:“在一名患有髓过氧化物酶缺乏症的日本患者中发现了新的错义突变。”基因。
- DOI:
- 发表时间:
- 期刊:
- 影响因子:0
- 作者:
- 通讯作者:
Saito, T., et al.: "Increase of Hepatic NKT Cells in LECT2-Deficient Mice Contributes to Severe Concanavalin A-Induced Hepatitis"Journal of Immunology. in press.
Saito, T., 等人:“LECT2 缺陷小鼠中肝 NKT 细胞的增加导致严重伴刀豆球蛋白 A 诱导的肝炎”免疫学杂志。
- DOI:
- 发表时间:
- 期刊:
- 影响因子:0
- 作者:
- 通讯作者:
Saito, T., Okumura, A., Watanabe, H., Asano, M., Ishida-Okawara, A., Sakagami, J., Sudo, K., Hatano-Yokoe, Y., Bezbradica, JS, Joyce, S., Abo, T., Iwakura, Y., Suzuki, K., Yamagoe S.: "Increase of Hepatic NKT Cells in LECT2-Deficient Mice Contributes to S
Saito, T.、Okumura, A.、Watanabe, H.、Asano, M.、Ishida-Okawara, A.、Sakagami, J.、Sudo, K.、Hatano-Yokoe, Y.、Bezbradica, JS、Joyce,
- DOI:
- 发表时间:
- 期刊:
- 影响因子:0
- 作者:
- 通讯作者:
N, Sakai, et al.: "Involvement of histone acetylation in ovarian steroid-induced deciduali-Zation of human endometrial stromal cells"J. Biol. Chem.. (in press).
N,Sakai 等人:“组蛋白乙酰化参与卵巢类固醇诱导的人子宫内膜基质细胞的蜕膜化”J。
- DOI:
- 发表时间:
- 期刊:
- 影响因子:0
- 作者:
- 通讯作者:
Ovejero C., et al.: "Identification of the leukocyte cell-derived chemotaxin2 (LECT2) as a direct target gene of s-catenin in the liver"Hepatology. in press.
Ovejero C. 等人:“鉴定白细胞来源的趋化因子 2 (LECT2) 作为肝脏中 s-连环蛋白的直接靶基因”肝病学。
- DOI:
- 发表时间:
- 期刊:
- 影响因子:0
- 作者:
- 通讯作者:
{{
item.title }}
{{ item.translation_title }}
- DOI:
{{ item.doi }} - 发表时间:
{{ item.publish_year }} - 期刊:
- 影响因子:{{ item.factor }}
- 作者:
{{ item.authors }} - 通讯作者:
{{ item.author }}
数据更新时间:{{ journalArticles.updateTime }}
{{ item.title }}
- 作者:
{{ item.author }}
数据更新时间:{{ monograph.updateTime }}
{{ item.title }}
- 作者:
{{ item.author }}
数据更新时间:{{ sciAawards.updateTime }}
{{ item.title }}
- 作者:
{{ item.author }}
数据更新时间:{{ conferencePapers.updateTime }}
{{ item.title }}
- 作者:
{{ item.author }}
数据更新时间:{{ patent.updateTime }}
YAMAGOE Satoshi其他文献
YAMAGOE Satoshi的其他文献
{{
item.title }}
{{ item.translation_title }}
- DOI:
{{ item.doi }} - 发表时间:
{{ item.publish_year }} - 期刊:
- 影响因子:{{ item.factor }}
- 作者:
{{ item.authors }} - 通讯作者:
{{ item.author }}
{{ truncateString('YAMAGOE Satoshi', 18)}}的其他基金
Analysis of repression mechanism by LECT2 in rheumatoid arthritis
LECT2对类风湿性关节炎的抑制机制分析
- 批准号:
20591180 - 财政年份:2008
- 资助金额:
$ 2.37万 - 项目类别:
Grant-in-Aid for Scientific Research (C)
相似海外基金
Development of platform for non-invasive, rapid, and simple analysis of cytokine secretion from single cells
开发非侵入、快速、简单分析单细胞细胞因子分泌的平台
- 批准号:
23H01824 - 财政年份:2023
- 资助金额:
$ 2.37万 - 项目类别:
Grant-in-Aid for Scientific Research (B)
Therapeutic approach to confer an anti-tumorigenic role of IL-6 to the pro-tumorigenic cytokine in effector T cells
赋予效应T细胞中促肿瘤细胞因子IL-6抗肿瘤作用的治疗方法
- 批准号:
23K06723 - 财政年份:2023
- 资助金额:
$ 2.37万 - 项目类别:
Grant-in-Aid for Scientific Research (C)
Diversity of haematopoietic cytokine receptor activation exploring with marine probes
用海洋探针探索造血细胞因子受体激活的多样性
- 批准号:
23K14019 - 财政年份:2023
- 资助金额:
$ 2.37万 - 项目类别:
Grant-in-Aid for Early-Career Scientists
A point-of-care salivary cytokine test for early detection of oral cancer
用于早期发现口腔癌的即时唾液细胞因子检测
- 批准号:
10760626 - 财政年份:2023
- 资助金额:
$ 2.37万 - 项目类别:
Development of Coccidioides Cytokine Release Assay
球孢子菌细胞因子释放测定的发展
- 批准号:
10760131 - 财政年份:2023
- 资助金额:
$ 2.37万 - 项目类别:
Combinatorial cytokine-coated macrophages for targeted immunomodulation in acute lung injury
组合细胞因子包被的巨噬细胞用于急性肺损伤的靶向免疫调节
- 批准号:
10648387 - 财政年份:2023
- 资助金额:
$ 2.37万 - 项目类别:
FOXA1 regulates cytokine signaling and immune landscape in prostate cancer through ARID1A
FOXA1 通过 ARID1A 调节前列腺癌中的细胞因子信号传导和免疫景观
- 批准号:
10681898 - 财政年份:2023
- 资助金额:
$ 2.37万 - 项目类别:
Cytokine Regulation of Secondary Neural Progenitors
次级神经祖细胞的细胞因子调节
- 批准号:
10752901 - 财政年份:2023
- 资助金额:
$ 2.37万 - 项目类别:
Generating a novel conditional knockout mouse for a super-enhancer that controls cytokine responsiveness
生成一种新型条件敲除小鼠,用于控制细胞因子反应的超级增强子
- 批准号:
10740932 - 财政年份:2023
- 资助金额:
$ 2.37万 - 项目类别:
Cytokine receptor common beta chain alterations in mediastinal lymphomas
纵隔淋巴瘤细胞因子受体常见β链改变
- 批准号:
489197 - 财政年份:2023
- 资助金额:
$ 2.37万 - 项目类别:
Operating Grants