Molecular cloning of a novel kinin-forming enzyme and its tissue expression in the human cardiovascular organ

新型激肽形成酶的分子克隆及其在人体心血管器官中的组织表达

• 批准号: 13670774
• 负责人: NAKAHIMA Yoshiyuki
• 金额: $ 2.24万
• 依托单位: Fukuoka Dental College (2002)Fukuoka College of Health Sciences (2001)
• 依托单位国家: 日本
• 项目类别: Grant-in-Aid for Scientific Research (C)
• 财政年份: 2001
• 资助国家: 日本
• 起止时间: 2001 至 2002
• 项目状态: 已结题
• 来源: https://kaken.nii.ac.jp/grant/KAKENHI-PROJECT-13670774/
• 关键词: Kallikrein; Kinin; cloning; human tissues

AbstractWe previously purified a novel kallikrein-like enzyme from the dog heart and demonstrated that it is not only able to form kinins but can also convert angiotensin (Ang) I to Ang II. To clarity whether this novel kallikrein-like enzyme is present in human tissue, we tried to clone this enzyme. In the present study, we have subcloned a kallikrein gene from the human heart and neutrophil cDNA library by using degenerate oligonucleotide and nested PCR amplification. The transcript is identical with the partial sequence of a human renal kallikrein gene. So far, we have not cloned a kallikrein gene from the human heart and neutrophil cDNA library by using this transcript as a target DNA. These results suggest that a tissue kallikrein exerts a variety of biological effects not only in renal tissue, but also human heart and neutrophils. However, it remains unresolved whether this enzyme is novel.

我们以前从狗心脏中纯化了一种新的激肽释放酶样酶，并证明它不仅能够形成激肽，而且还可以将血管紧张素（Ang）I转化为Ang II。为了明确这种新型的激肽释放酶样酶是否存在于人体组织中，我们试图克隆这种酶。本研究采用简并寡核苷酸和巢式PCR技术，从人心脏和中性粒细胞cDNA文库中亚克隆了激肽释放酶基因。该转录物与人肾激肽释放酶基因的部分序列相同。到目前为止，我们还没有从人心脏和中性粒细胞cDNA文库中克隆到激肽释放酶基因，通过使用该转录本作为靶DNA。这些结果表明，组织激肽释放酶不仅在肾组织中，而且在人心脏和中性粒细胞中发挥多种生物学效应。然而，这种酶是否是新的仍然没有解决。