Studies on the expression and function of basigin (CD147) in human epidermal keratinocytes

人表皮角质形成细胞中basigin（CD147）的表达及功能研究

• 批准号: 13670894
• 负责人: KANZAKI Tamotsu
• 金额: $ 1.92万
• 依托单位: Kagoshima University
• 依托单位国家: 日本
• 项目类别: Grant-in-Aid for Scientific Research (C)
• 财政年份: 2001
• 资助国家: 日本
• 起止时间: 2001 至 2003
• 项目状态: 已结题
• 来源: https://kaken.nii.ac.jp/grant/KAKENHI-PROJECT-13670894/
• 关键词: Basigin; keratinocyte; differentiation; epidermal skin cancer; immunohistochemistry; immuno-electron microscopy; 表皮細胞の分化; Basigin; 上皮系皮膚腫瘍

Basigin is a glycosylated transmembrane protein belonging to the immunoglobulin superfamily and is thought to be associated with cell development and differentiation.In the present study, we investigated the expression of basigin in relation with differentiation of normal keratinocytes and in benign and malignant tumors arisen from keratinocytes. Basigin expression was analyzed immunohistochemically in 20 cases of verruca vulgaris (VV), various benign, premalignant and malignant epidermal tumors including 21 cases of seborrheic keratosis (SK), 20 of actinic keratosis (AK), 20 of Bowen's disease (BD) and 57 of squamous cell carcinoma. SCCs were classified using Broder's system of grading. Basigin is expressed in the basal cells of normal epidermis but not in prickle and granular cells. All 20 VVs and 21 SKs showed the same staining pattern of basigin as normal epidermis. Four of 20 Aks and 6 of 19 BDs exhibited positive staining throughout the lesion. Eight of 20 grade 1 SCCs showed pos … More itive staining at the periphery of tumor nests. Sixteen of 20 grade 2 SCCs and all of the 17 grade 3 SCCs expressed CD147 throughout tumor nests. Grade 2 SCCs and 12 of 17 grade 3 SCCs showed positive staining and 5 of 17 grade 3 SCCs exhibited strongly positive staining. These results indicate that basigin is expressed in cells which have potential to differentiate and proliferate such as basal cells and poorly differentiated cancer cells. Based on these observations, we propose that basigin has close association with keratinocytes differentiation.Next, in order to examine possible function of basigin in keratinocytes differentiation, the Ultrastructural localization of basigin in normal human epidermal keratinocytes was investigated by immuno-electron microscopy. The basigin labeling was strongest on membranes of basal cells, weaker on prickle cells, and absent in granular and horny cells. On the membrane of basal cells, labeling was observed on the apical and lateral sides but not on the dermal side. Gold particles were mostly observed on the surface of microvilli, especially on their tips. There were fewer on the intermicrovillous membrane and they were absent on the desmosome. These results are consistent with our previous report that basigin expression is correlated with differentiation of epidermal keratinocytes and further imply that microvilli on basal and suprabasal keratinocytes might play roles in the differentiation of keratinocytes through basigin on the tips of microvilli. Less

Basigin是一种糖基化的跨膜蛋白，属于免疫球蛋白超家族，被认为与细胞的发育和分化有关。在本研究中，我们研究了Basigin的表达与正常角质形成细胞的分化和良性和恶性肿瘤角质形成细胞引起的。应用免疫组织化学方法检测了20例寻常疣（VV）、21例脂溢性角化病（SK）、20例光化性角化病（AK）、20例Bowen病（BD）和57例鳞状细胞癌中Basigin的表达。SCC采用Broder分级系统进行分类。Basigin在正常表皮的基底细胞中表达，但在棘细胞和颗粒细胞中不表达。所有20个VV和21个SK均显示与正常表皮相同的basigin染色模式。20例Aks中有4例和19例BD中有6例在整个病变中显示阳性染色。20例1级SCC中8例为阳性， ...更多信息 在肿瘤巢的周围有阳性染色。20个2级SCC中的16个和所有17个3级SCC在整个肿瘤巢中表达CD147。2级SCC和17例3级SCC中12例呈阳性染色，17例3级SCC中5例呈强阳性染色。这些结果表明，basigin在具有分化和增殖潜力的细胞中表达，例如基底细胞和低分化癌细胞。基于这些观察结果，我们认为basigin与角质形成细胞的分化密切相关。接下来，为了研究basigin在角质形成细胞分化中的可能功能，我们用免疫电镜观察了basigin在正常人表皮角质形成细胞中的超微结构定位。basigin标记是最强的基底细胞膜上，棘细胞上较弱，颗粒和角质细胞缺席。在基底细胞膜上，在顶侧和侧面观察到标记，但在真皮侧未观察到标记。金颗粒主要分布在微绒毛表面，尤其是微绒毛顶端。在微绒毛间膜上有少量的，在桥粒上没有。这些结果与我们先前的报告一致，即basigin表达与表皮角质形成细胞的分化相关，并进一步暗示基底和基底上角质形成细胞上的微绒毛可能通过微绒毛顶端的basigin在角质形成细胞的分化中发挥作用。少

项目成果

Chen X, Kanekura T, Zhang GY.: "Expression of matrix metalloproteinases in metastatic lesions of cutaneous squamous cell carcinoma."Hunan Yi Ke Da Xue Due Bao. 26. 297-300 (2001)

陈X，Kanekura T，张GY.：“基质金属蛋白酶在皮肤鳞状细胞癌转移灶中的表达。”湖南医科大学应报。

Kanekura T, Chen X, Kanzaki T.: "Basigin (CD147) is expressed on melanoma cells and induces tumor cell invasion by stimulating production of matrix metalloproteinases by fibroblasts"Int J Cancer. 99(4). 520-528 (2002)

Kanekura T、Chen X、Kanzaki T.：“Basigin (CD147) 在黑色素瘤细胞上表达，并通过刺激成纤维细胞产生基质金属蛋白酶来诱导肿瘤细胞侵袭”Int J Cancer。

Kanekura T, Chen X, Kanzaki T.: "Basigin (CD147) is expressed on melanoma cells and induces tumor cell invasion by stimulating production of matrix metalloproteinases by fibroblasts."Int J Cancer. 99. 520-528 (2002)

Kanekura T、Chen X、Kanzaki T.：“Basigin (CD147) 在黑色素瘤细胞上表达，并通过刺激成纤维细胞产生基质金属蛋白酶来诱导肿瘤细胞侵袭。”Int J Cancer。

Chen Xiang, Kanekura Takuro, Zhang Gui-ying, et al.: "Expression of matrix metalloproteinases in metastatic lesions of cutaneous squamous cell carcinoma"Bull.Hunan.Med.Uuniv.(湖南医科大学学報). 26巻4号. 297-304 (2001)

陈翔，Takuro Kanekura，张桂英，等：“皮肤鳞状细胞癌转移性病变中的表达”，湖南医科大学学报，第 26 期。 4. 297-304 (2001)

Chen X, Kanekura T, Tsuyama S, Murata F, Kanzaki T.: "Ultrastructural localization of basigin in normal human epidermis."Histochem Cell Biol. 115. 465-470 (2001)

Chen X、Kanekura T、Tsuyama S、Murata F、Kanzaki T.：“正常人表皮中basigin的超微结构定位。”组织化学细胞生物学。