3-D display of the peripheral microstructure of the lung with synchrotron radiation CT

同步辐射 CT 3D 显示肺周围微结构

• 批准号: 13670946
• 负责人: YASUHARA Yoshifumi
• 金额: $ 2.18万
• 依托单位: Ehime University
• 依托单位国家: 日本
• 项目类别: Grant-in-Aid for Scientific Research (C)
• 财政年份: 2001
• 资助国家: 日本
• 起止时间: 2001 至 2002
• 项目状态: 已结题
• 来源: https://kaken.nii.ac.jp/grant/KAKENHI-PROJECT-13670946/
• 关键词: CT, Lung; CT, Ultra high-resolution; peripheral pulmonary structure; three-dimension; CT, 肺; CT, 超高分解能; CT、肺; CT、超高分解能

[Purpose] 3-D microstructure of the lung (peripheral structures beyond respiratory bronchiole) is extremely difficult to visualize, because of the limitation of spatial resolution of CT (300 micrometer even with high resolution CT). In this study, we try to visualize 3-D display of the peripheral microstructures of the lung using the volume data with synchrotron radiation CT (SRCT) of the lung specimen.[Materials and Methods] Lung specimens of normal lung and several disease processes were obtained at autopsy, and were inflated and fixed by Heitzman's method. Each specimen was cut down to cylindrical sample specimen with 6-mm diameter and 20 mm height. SRCT images of the sample specimens were obtained with SRCT system constructed at synchrotron radiation facility (Super Photon Ring 8.0 GeV, Hyogo, Japan). SRCT provides isotropic volume data (spatial resolution 12 micrometer), with serial 815 slices and each slice with 800 x 800 pixels. 3-D microstructures were constructed with automatic segmentation and combination of the surface and volume rendering display technique on the workstation (Sun SPARCstation), Several 3-D models of the air flow could be illustrated with animation as air came into the peripheral airway and air space.[Results] 3-D display of the peripheral microstructure of the lung was established precisely. 3-D models of the peripheral airflow could represent "airflow" and "diffusion". Conducting parts between terminal bronchiole and peripheral bronchiole were narrowest in the airway. The next peripheral portions were expanded to branch to second respiratory bronchioles.[Conclusion] 3-D display of the peripheral pulmonary structure and 3-D models of the peripheral air flow with synchrotron radiation CT enable us to study the morphology, physiology, and pathophysiology of the peripheral respiratory system non-destructively.

[目的]由于CT空间分辨率的限制（即使是高分辨率CT也只有300 μ m），肺的三维显微结构（呼吸性细支气管以外的周围结构）很难显示。在这项研究中，我们尝试使用同步辐射CT（SRCT）的肺标本的体积数据的三维显示肺的周边微结构。[材料与方法]尸检时取正常肺和几种病变肺标本，用Heitzman法充气固定。将每个试样切割成直径为6 mm、高度为20 mm的圆柱形试样。用同步辐射装置（Super Photon Ring 8.0 GeV，Hyogo，Japan）的SRCT系统获得样品的SRCT图像。SRCT提供各向同性体积数据（空间分辨率12微米），具有连续815个切片，每个切片具有800 x 800像素。在SunSPARCstation工作站上，采用表面和体绘制技术相结合的自动分割技术，建立了三维微结构，并以动画方式显示了空气进入周围气道和空气间隙时的气流三维模型。[结果]建立了精确的肺周边显微结构三维显示。外围气流的3-D模型可以表示“气流”和“扩散”。终末细支气管与周围细支气管之间的传导部位在气道内呈环状。下一个外周部分扩张至第二呼吸性细支气管的分支。[结论]同步辐射CT三维显示周围肺结构和周围气流的三维模型，可以无损地研究周围呼吸系统的形态、生理和病理生理。

项目成果

Shimizu K, Ikura H, Ikezoe J, Nagareda T, Yagi N: "Synchrotron radiation microtomography of the human lung tissue specimens : three-dimensional display of peripheral air way and air space, and visualization of ventilation"THE LUNG perspectives. 10 (2). 14

Shimizu K、Ikura H、Ikezoe J、Nagareda T、Yagi N：“人体肺组织标本的同步辐射显微断层扫描：外周气道和空气空间的三维显示以及通气的可视化”THE LUNG 观点。